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![Western Blot: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Western-Blot-NB120-2788-img0019.jpg "Western Blot: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.")
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using Monoclonal antibody (13D3) shows staining in NIH-3T3 cells. HSC70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing HSC70 at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0013.jpg)
![Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Prostate carcinoma tissue.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunohistochemistry-Paraffin-NB120-2788-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in HeLa cells. HSC70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing HSC70 at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in MCF-7 cells. HSC70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing HSC70 at a dilution of 1:20-1:200 C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0012.jpg)
![Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunohistochemistry-Paraffin-NB120-2788-img0020.jpg)
![Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Testis tissue.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunohistochemistry-Paraffin-NB120-2788-img0021.jpg)
![Immunoprecipitation: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug whole cell lysate with 2ul of HSC70 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSC70 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with goat anti-mouse IgM-HRP secondary antibody for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunoprecipitation-NB120-2788-img0023.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Western-Blot-NBP1-97868-img0002.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Immunohistochemistry-Paraffin-NBP1-97868-img0001.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Western-Blot-NB100-41377-img0006.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41377-img0005.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NB100-41377-img0008.jpg)