| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1072AF405 | |||
| Alexa Fluor 488 | NB21-1072AF488 | |||
| Alexa Fluor 647 | NB21-1072AF647 | |||
| Alexa Fluor 700 | NB21-1072AF700 | |||
| Biotin | NB21-1072B | |||
| DyLight 405 | NB21-1072V | |||
| DyLight 488 | NB21-1072G | |||
| DyLight 550 | NB21-1072R | |||
| DyLight 650 | NB21-1072C | |||
| FITC | NB21-1072F | |||
| HRP | NB21-1072H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1072SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1024AF405 | |||
| Alexa Fluor 488 | NB21-1024AF488 | |||
| Alexa Fluor 647 | NB21-1024AF647 | |||
| Alexa Fluor 700 | NB21-1024AF700 | |||
| Biotin | NB21-1024B | |||
| DyLight 405 | NB21-1024V | |||
| DyLight 488 | NB21-1024G | |||
| DyLight 550 | NB21-1024R | |||
| DyLight 650 | NB21-1024C | |||
| FITC | NB21-1024F | |||
| HRP | NB21-1024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1024SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-267SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1052AF405 | |||
| Alexa Fluor 488 | NB21-1052AF488 | |||
| Alexa Fluor 647 | NB21-1052AF647 | |||
| Alexa Fluor 700 | NB21-1052AF700 | |||
| Biotin | NB21-1052B | |||
| DyLight 405 | NB21-1052V | |||
| DyLight 488 | NB21-1052G | |||
| DyLight 550 | NB21-1052R | |||
| DyLight 650 | NB21-1052C | |||
| FITC | NB21-1052F | |||
| HRP | NB21-1052H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1052SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1188AF405 | |||
| Alexa Fluor 488 | NB21-1188AF488 | |||
| Alexa Fluor 647 | NB21-1188AF647 | |||
| Alexa Fluor 700 | NB21-1188AF700 | |||
| Biotin | NB21-1188B | |||
| DyLight 405 | NB21-1188V | |||
| DyLight 488 | NB21-1188G | |||
| DyLight 550 | NB21-1188R | |||
| DyLight 650 | NB21-1188C | |||
| FITC | NB21-1188F | |||
| HRP | NB21-1188H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1188SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1142AF405 | |||
| Alexa Fluor 488 | NB21-1142AF488 | |||
| Alexa Fluor 647 | NB21-1142AF647 | |||
| Alexa Fluor 700 | NB21-1142AF700 | |||
| Biotin | NB21-1142B | |||
| DyLight 405 | NB21-1142V | |||
| DyLight 488 | NB21-1142G | |||
| DyLight 550 | NB21-1142R | |||
| DyLight 650 | NB21-1142C | |||
| FITC | NB21-1142F | |||
| HRP | NB21-1142H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1142SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1383V | |||
| FITC | NB21-1383F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1383SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1132AF405 | |||
| Alexa Fluor 488 | NB21-1132AF488 | |||
| Alexa Fluor 647 | NB21-1132AF647 | |||
| Alexa Fluor 700 | NB21-1132AF700 | |||
| Biotin | NB21-1132B | |||
| DyLight 405 | NB21-1132V | |||
| DyLight 488 | NB21-1132G | |||
| DyLight 550 | NB21-1132R | |||
| DyLight 650 | NB21-1132C | |||
| FITC | NB21-1132F | |||
| HRP | NB21-1132H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1132SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1202AF405 | |||
| Alexa Fluor 488 | NB21-1202AF488 | |||
| Alexa Fluor 647 | NB21-1202AF647 | |||
| Alexa Fluor 700 | NB21-1202AF700 | |||
| Biotin | NB21-1202B | |||
| DyLight 405 | NB21-1202V | |||
| DyLight 488 | NB21-1202G | |||
| DyLight 550 | NB21-1202R | |||
| DyLight 650 | NB21-1202C | |||
| FITC | NB21-1202F | |||
| HRP | NB21-1202H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1202SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1021AF405 | |||
| Alexa Fluor 488 | NB21-1021AF488 | |||
| Alexa Fluor 647 | NB21-1021AF647 | |||
| Alexa Fluor 700 | NB21-1021AF700 | |||
| Biotin | NB21-1021B | |||
| DyLight 405 | NB21-1021V | |||
| DyLight 488 | NB21-1021G | |||
| DyLight 550 | NB21-1021R | |||
| DyLight 650 | NB21-1021C | |||
| FITC | NB21-1021F | |||
| HRP | NB21-1021H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1021SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1144AF405 | |||
| Alexa Fluor 488 | NB21-1144AF488 | |||
| Alexa Fluor 647 | NB21-1144AF647 | |||
| Alexa Fluor 700 | NB21-1144AF700 | |||
| Biotin | NB21-1144B | |||
| DyLight 405 | NB21-1144V | |||
| DyLight 488 | NB21-1144G | |||
| DyLight 550 | NB21-1144R | |||
| DyLight 650 | NB21-1144C | |||
| FITC | NB21-1144F | |||
| HRP | NB21-1144H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1144SS | |||
![Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Dot-Blot-NB21-1072-img0010.jpg "Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1072 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1072-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of Histone H3K9me2 on HeLa histone preps. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NB21-1072-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Histone H3.2 K9me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1072-img0008.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NB21-1072-img0012.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-59206-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - HeLa cells were stained with the antibody against H3K27me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59206-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - ChIP assays were performed using human K562 cells, the antibody against H3K27me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 100,000 cells. The indicated amounts of antibody were used per ChIP experiment. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and TSH2B and MYT1, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59206-img0003.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3 diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-59206-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - ChIP assays were performed using human K562 cells, the antibody against H3K27me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. The indicated amounts of antibody were used per ChIP experiment. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and TSH2B and MYT1, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59206-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K27me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:1,000,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-ELISA-NBP2-59206-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - To test the cross reactivity of the antibody against H3K27me3, a Dot Blot analysis was performed with peptides containing other modifications or unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Dot-Blot-NBP2-59206-img0005.jpg)
![Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Dot-Blot-NB21-1024-img0015.jpg "Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 [ac Lys4] Antibody [NB21-1024] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1024 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0019.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 K4ac1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0021.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in (1) HeLa histone prep and (2) NIH 3T3 histone prep lysates. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0016.jpg)
![Western Blot: Histone H3 Antibody [NB500-267] - Detection of Histone H3 in lysates using NB500-267. (1) MEF and (2) HeLa. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-267-img0003.jpg "Western Blot: Histone H3 Antibody [NB500-267] - Detection of Histone H3 in lysates using NB500-267. (1) MEF and (2) HeLa. Observed molecular weight is ~15 kDa.")
![Western Blot: Histone H3 Antibody [NB500-267] - Analysis of HeLa lysates using NB500-267. Image courtesy of Gregg Semenza, (PMID: 21620138). Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-267-img0004.jpg)
![Dot Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Dot-Blot-NB21-1052-img0011.jpg "Dot Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in picomoles of protein.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of NB21-1052 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1052-img0010.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in HeLa Histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1052-img0017.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in NIH 3T3 Histone prep lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0014.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0012.jpg)
![Dot Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - Dot blot analysis of Histone H3 pS28 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Dot-Blot-NB21-1188-img0003.jpg "Dot Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - Dot blot analysis of Histone H3 pS28 in picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody [NB21-1188] - Histone H3.2 pS28 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1188-img0007.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - WB analysis of Histone H3 pS28 in NIH 3T3 histone prep. Observed molecular weight is ~14 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Western-Blot-NB21-1188-img0002.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Analysis of H3K18me2 antibody using peptides stated above in 1.0, 0.3 and 0.1 picomoles.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Dot-Blot-NB21-1142-img0011.jpg "Dot Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Analysis of H3K18me2 antibody using peptides stated above in 1.0, 0.3 and 0.1 picomoles.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of NB21-1142 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Chromatin-Immunoprecipitation-NB21-1142-img0010.jpg)
![Western Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Analysis of H3.2 K18me2 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Western-Blot-NB21-1142-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1142-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Detection of Histone H3 [dimethyl Lys18] in NIH 3T3 histone preps. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Western-Blot-NB21-1142-img0012.jpg)
![Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 in (1) HeLa Histone prep (2) 3T3 Histone prep, and (3) C.elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Western-Blot-NB21-1383-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 in (1) HeLa Histone prep (2) 3T3 Histone prep, and (3) C.elegans embryo lysate. Observed molecular weight is ~16 kDa.")
![Dot Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Dot-Blot-NB21-1383-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1383 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NB21-1383-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Immunofluorescence: [NB21-1383] - H3 K79me3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Immunocytochemistry-NB21-1383-img0009.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of HIstone H3 R17me2a in NIH 3T3 histone prep cell lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Western-Blot-NB21-1132-img0011.jpg "Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of HIstone H3 R17me2a in NIH 3T3 histone prep cell lysate. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - 2 ug of NB21-1132 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Chromatin-Immunoprecipitation-NB21-1132-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Histone H3.2 R17me2a antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1132-img0013.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of Histone H3 R17me2a in C. elegans embryo lysate. Observed molecular weight is ~10 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Western-Blot-NB21-1132-img0010.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone H3 R2me2s/K4Me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1202-img0012.jpg "Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone H3 R2me2s/K4Me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1202 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Chromatin-Immunoprecipitation-NB21-1202-img0013.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of H3R2me2s/K4Me2 in picomoles of peptide. Lanes (1) R2Me2 only, (2) Both Modifications, and (3) K4Me2.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Dot-Blot-NB21-1202-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Histone H3.2 R2me2s/K4me2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 594 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1202-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone R2 in NIH 3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1202-img0011.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0002.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Histone H3.2 K4me1 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Immunocytochemistry-NB21-1021-img0001.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - H3K4Me1 immunoblot in Jurkat cells. Observed molecular weight is ~17 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of H3K4me1 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0003.jpg)
![Dot Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Dot-Blot-NB21-1144-img0005.jpg "Dot Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in the peptides listed above in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody [NB21-1144] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1144 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NB21-1144-img0010.jpg)
![Western Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in (1) HeLa histone prep, (2) NIH 3T3 histone prep and (3) C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Western-Blot-NB21-1144-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys18] Antibody [NB21-1144] - Histone H3 [ac Lys18] antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1144-img0009.jpg)