| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF276-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF527-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2480-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3581-SP | |||
| Carrier Free | MAB3581-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3950-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-44309SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4059-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4140-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3583-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5694-SP | |||
![C. albicans induces c-Met, EGFR, and E-cadherin to form a multiprotein complex.(A-C) Proximity ligation assay showing the interaction of c-Met with E-cadherin, EGFR with E-cadherin, and c-Met with EGFR in oral epithelial cells with and without 20-min infection with C. albicans. Confocal microscopic images (A). Scale bar 10 μm. Signal counts (B). Proximity ligation assay showing the interaction of c-Met with E-cadherin, EGFR with E-cadherin, and c-Met with EGFR in oral epithelial cells with and without 20-min infection with C. albicans. (A) Confocal microscopic images. Scale bar 10 μm. (B) Signal counts. (C-E) Co-immunoprecipitation experiments in oral epithelial cells transfected with control or E-cadherin (E-cad) siRNA and then infected with C. albicans for 20 min. (C) Representative immunoblots of proteins obtained by immunoprecipitation with an anti-c-Met antibody. (D and E) Densitometric analysis of 5 immunoblots. Results are mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001, ns; not significant (two-tailed Student’s t test [B] or one-way ANOVA with Sidak’s multiple comparisons test [D and E]). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37611070), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af276_human-hgf-r-c-met-affinity-purified-polyclonal-ab-8120255561322.jpg)
![Functional interactions among c-Met, EGFR, and E-cadherin during the endocytosis of C. albicans.(A-C) Immunoblot analysis showing the effects of the EGFR inhibitor gefitinib and the c-Met inhibitor SGX523 on C. albicans-induced phosphorylation of c-Met and EGFR in oral epithelial cells after 20 min of infection. Representative immunoblot (A), densitometric analysis of 4 immunoblots showing the phosphorylation of EGFR (B) and c-Met (C). Data are mean ± SD. (D) Effects of SGX523 and gefitinib on the endocytosis of C. albicans by oral epithelial cells. (E and F) Endocytosis of C. albicans by NIH/3T3 cells that expressed human c-Met (E) or human c-Met, EGFR, and HER2 (F). (G) Confocal micrographs of NIH3T3 cells expressing no human receptors (control), human c-Met, human EGFR and HER2, or human c-Met, EGFR, and HER2. The cells were infected with wild-type C. albicans germ tubes for 20 min, after which phosphorylated c-Met was detected with a phosphospecific anti-c-Met antibody. Scale bar 10 μm. Results in (D-F) are the mean ± SD of three experiments, each performed in triplicate. *p < 05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns; not significant (one-way ANOVA with Sidak’s multiple comparisons test [B-D] or two-tailed Student’s t test [E and F]). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37611070), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af276_human-hgf-r-c-met-affinity-purified-polyclonal-ab-812025555319.jpg)
![C. albicans activates c-Met in oral epithelial cells.(A) Confocal microscopic images of OKF6/TERT-2 oral epithelial cells infected with C. albicans SC5314 for 20 min. c-Met, the epidermal growth factor receptor (EGFR), and E-cadherin were detected by indirect immunofluorescence using specific antibodies. Arrows point to the organisms in the magnified insets. Scale bar 10 μm. (B and C) Immunoblot analysis showing the time course of the phosphorylation of c-Met and EGFR in oral epithelial cells induced by C. albicans germ tubes (B). Densitometric analysis of 4 immunoblots (C). Data are mean ± SD. (D and E) Knockdown of c-Met with siRNA (D) or inhibition of c-Met signaling with SG523 (E) in oral epithelial cells inhibits the endocytosis of C. albicans. (F) Stimulation of oral epithelial cells with recombinant hepatocyte growth factor (HGF) enhances the endocytosis of C. albicans. Data in (D-F) are the mean ± SD of three experiments, each performed in triplicate. **p < 0.01, ****p < 0.0001, ns; not significant (two-tailed Student’s t test [D and E] or one-way ANOVA with Sidak’s multiple comparisons test [F]). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37611070), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af276_human-hgf-r-c-met-affinity-purified-polyclonal-ab-812025555349.jpg)
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [556]. HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [556]. HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556].](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0004.jpg)
![Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (556) [NBP2-43641] - Human esophageal carcinoma. c-Met antibody [556] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Immunohistochemistry-Paraffin-NBP2-43641-img0007.jpg)
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0001.jpg)
![Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (556) [NBP2-43641] - Human breast carcinoma. c-Met antibody [556] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Immunohistochemistry-Paraffin-NBP2-43641-img0005.jpg)
![Immunoprecipitation: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of MET protein from HeLa whole cell extracts using 5 ug of MET antibody [556]. Western blot analysis was performed using MET antibody [556]. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Immunoprecipitation-NBP2-43641-img0003.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-26112025182908.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251843456.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251850424.jpg)
![Immunocytochemistry/Immunofluorescence: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44309-img0003.jpg "Immunocytochemistry/Immunofluorescence: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - IHC analysis of HGF R/c-Met (1G7NB) in human liver cancer.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-44309-img0004.jpg)
![Flow (Intracellular): HGF R/c-MET Antibody (1G7NB) [Azide Free] [NBP2-44309] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Flow-Intracellular-NBP2-44309-img0002.jpg)
