G-CSF Antibodies

MAB2141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human G‑CSF Monoclonal Antibody (Catalog # MAB11296) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB2141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human G‑CSF Monoclonal Antibody (Catalog # MAB11296) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human G‑CSF protein was serially diluted 2-fold and captured by Mouse Anti-Human G‑CSF Monoclonal Antibody (MAB2141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human G‑CSF Monoclonal Antibody (Catalog # MAB11296) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

204-IL), Recombinant Human GM-CSF (50 ng/ml; 215-GM), LPS (20 ng/ml), Recombinant Human TNF-alpha (20 ng/ml; 210-TA), and Recombinant Human IL-beta (20 ng/ml; 201-LB) for 6 days to differentiate into Dendritic Cells were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (IC002P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_lightbox"> 204-IL), Recombinant Human GM-CSF (50 ng/ml; 215-GM), LPS (20 ng/ml), Recombinant Human TNF-alpha (20 ng/ml; 210-TA), and Recombinant Human IL-beta (20 ng/ml; 201-LB) for 6 days to differentiate into Dendritic Cells were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (IC002P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " alt="Human CD14⁺ PBMC treated with Recombinant Human IL-4 (20 ng/ml; 204-IL), Recombinant Human GM-CSF (50 ng/ml; 215-GM), LPS (20 ng/ml), Recombinant Human TNF-alpha (20 ng/ml; 210-TA), and Recombinant Human IL-beta (20 ng/ml; 201-LB) for 6 days to differentiate into Dendritic Cells were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (IC002P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_thumb" />

210-TA), and Recombinant Human IL-beta (Catalog # 201-LB) were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> 210-TA), and Recombinant Human IL-beta (Catalog # 201-LB) were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human G-CSF Monoclonal Antibody (Catalog # MAB214R). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_lightbox"> 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human G-CSF Monoclonal Antibody (Catalog # MAB214R). The ND₅₀ is typically 0.01-0.03 µg/mL." alt="Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human G-CSF Monoclonal Antibody (Catalog # MAB214R). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_thumb" />

IC002A, open histogram). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> IC002A, open histogram). View our protocol for Staining Intracellular Molecules." alt="Human macrophages treated with LPS, TNF-a and IL-1 beta was stained with Mouse Anti-Human G-CSF APC-conjugated Monoclonal Antibody (Catalog # IC2141A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Intracellular Molecules." class="big_thumb" />