FAM50A Antibodies
We offer FAM50A Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each FAM50A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our FAM50A Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the FAM50A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our FAM50A polyclonal antibodies and browse our FAM50A monoclonal antibody catalog.
Alternate Names for FAM50A Antibodies
anti-FAM50A antibody, anti-DXS9928EXAP-5 protein antibody, anti-family with sequence similarity 50, member A antibody, anti-HXC-26 antibody, anti-Protein HXC-26 antibody, anti-Protein XAP-5 antibody, anti-XAP5HXC26,9F antibody
7 Results for "fam50a" in Primary Antibodies
![Western Blot: FAM50A Antibody [NBP1-89344] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Western-Blot-NBP1-89344-img0012.jpg "Western Blot: FAM50A Antibody [NBP1-89344] - Analysis in human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: FAM50A Antibody [NBP1-89344] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89344-img0006.jpg)
![Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344] - Staining of human prostate shows strong nuclear/ cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Immunohistochemistry-Paraffin-NBP1-89344-img0014.jpg)
![Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Immunohistochemistry-Paraffin-NBP1-89344-img0001.jpg)
![Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344] - Staining of human cerebral cortex strong nuclear/cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Immunohistochemistry-Paraffin-NBP1-89344-img0009.jpg)
![Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Immunohistochemistry-Paraffin-NBP1-89344-img0010.jpg)
![Western Blot: FAM50A Antibody [NBP1-89344] - FAM50A interacts with U5 & C-complex proteins.a Schematic representation of the two-step splicing reaction (adapted from ref. 70). EFTUD2 (U5); FAM50A & DDX41 (C-complex). b Graphical representation of semi-native co-immunoprecipitation assay. Candidate interactors were overexpressed in U-87 cells, harvested in immunoprecipitation (IP) lysis buffer at 60 h post-transfection & immunoprecipitated with anti-FAM50A antibody. The interaction partners were detected in IP lysate using anti-V5 & anti-FAM50A antibodies, respectively. c, d Western blot of proteins after co-immunoprecipitation. Top: total protein lysate input (50 μg/lane) was migrated on 4–15% polyacrylamide gels to detect EFTUD2 (c) or DDX41 (d) using anti-V5 mouse monoclonal antibody. GAPDH was used as loading control. Bottom: Anti-FAM50A antibody was used to immunoprecipitate native FAM50A protein in total input lysate of 2.3 mg/condition (c) or 3.5 mg/condition (d). The IP lysate was separated in two parts (20% & 80%) & migrated independently on 4–15% polyacrylamide gels. The proteins of interest with predicated band sizes are indicated with black arrows. Plus & minus signs indicate presence & absence of relevant plasmids, respectively. EFTUD2 & DDX41 were detected in independent experiments using protein lysates derived from replicate batches of U-87 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32703943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89344_rabbit-polyclonal-fam50a-antibody-310202416113537.jpg)
![Western Blot: FAM50A Antibody (5F10) [H00009130-M02] - FAM50A monoclonal antibody (M02), clone 5F10. Analysis of FAM50A expression in PC-12.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Western-Blot-H00009130-M02-img0008.jpg "Western Blot: FAM50A Antibody (5F10) [H00009130-M02] - FAM50A monoclonal antibody (M02), clone 5F10. Analysis of FAM50A expression in PC-12.")
![Immunohistochemistry-Paraffin: FAM50A Antibody (5F10) [H00009130-M02] - Analysis of monoclonal antibody to FAM50A on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Immunohistochemistry-Paraffin-H00009130-M02-img0006.jpg)
![Western Blot: FAM50A Antibody (5F10) [H00009130-M02] - FAM50A monoclonal antibody (M02), clone 5F10 Analysis of FAM50A expression in Hela S3 NE.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Western-Blot-H00009130-M02-img0009.jpg)
![Sandwich ELISA: FAM50A Antibody (5F10) [H00009130-M02] - Detection limit for recombinant GST tagged FAM50A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Sandwich-ELISA-H00009130-M02-img0007.jpg)
![Western Blot: FAM50A Antibody (JE65-21) [NBP3-32332] - Western blot analysis of FAM50A on different lysates with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/1,000 dilution. Lane 1: U87MG cell lysate Lane 2: U937 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32332) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32332_rabbit-fam50a-mab-je65-21-278202415295523.jpg "Western Blot: FAM50A Antibody (JE65-21) [NBP3-32332] - Western blot analysis of FAM50A on different lysates with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/1,000 dilution. Lane 1: U87MG cell lysate Lane 2: U937 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32332) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FAM50A Antibody (JE65-21) [NBP3-32332] - Immunohistochemical analysis of paraffin-embedded rat rectum tissue with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32332) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32332_rabbit-fam50a-mab-je65-21-29820241435259.jpg)
![Immunocytochemistry/ Immunofluorescence: FAM50A Antibody (JE65-21) [NBP3-32332] - Immunocytochemistry analysis of NCI-H441 cells labeling FAM50A with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32332_rabbit-fam50a-mab-je65-21-29202410234281.jpg)
![Western Blot: FAM50A Antibody (SR1667) [NBP3-22602] - Western blot analysis using NBP3-22602 in (1) HeLa cell lysate; (2) Raw264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22602_rabbit-fam50a-mab-sr1667-5720231595520.jpg "Western Blot: FAM50A Antibody (SR1667) [NBP3-22602] - Western blot analysis using NBP3-22602 in (1) HeLa cell lysate; (2) Raw264.7 cell lysate.")
![Western Blot: FAM50A Antibody [NBP1-33494] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Western-Blot-NBP1-33494-img0002.jpg "Western Blot: FAM50A Antibody [NBP1-33494] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: FAM50A Antibody [NBP3-10708] - Western blot analysis of FAM50A in 293T Whole Cell lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/FAM50A-Antibody-Western-Blot-NBP3-10708-img0001.jpg "Western Blot: FAM50A Antibody [NBP3-10708] - Western blot analysis of FAM50A in 293T Whole Cell lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: FAM50A Antibody [H00009130-D01P] - Analysis of FAM50A expression in transfected 293T cell line by FAM50A polyclonal antibody.Lane 1: FAM50A transfected lysate(40.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FAM50A-Antibody-Western-Blot-H00009130-D01P-img0002.jpg "Western Blot: FAM50A Antibody [H00009130-D01P] - Analysis of FAM50A expression in transfected 293T cell line by FAM50A polyclonal antibody.Lane 1: FAM50A transfected lysate(40.20 KDa).Lane 2: Non-transfected lysate.")