Product Search for b7 h1 pd l1 cd274: Novus Biologicals

AF1019

PD-L1 Antibody [Unconjugated]

(3 Reviews)

100 μg

AF1019

HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

<b>Biological Strategies Validation. </b>RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0108). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

F0108). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

<b>Biological Strategies Validation. </b>Pyruvate kinase isoform M2 (PKM2) is required for LPS-induced expression of PD-L1. RAW cells (0.5 × 106 cells/ml) (A), bone marrow-derived macrophages (BMDMs) (B), or bone marrow-derived dendritic cells (C) were treated with TEPP-46 at 50 µM for 1 h prior to stimulation with LPS (100 ng/ml, 24 h), lysed and assayed for expression of PD-L1 by flow cytometry [(A–C) top panels] and pdl1 mRNA by RT-PCR [(A,B) bottom panels]. Statistical analysis was performed on cells from three separate experiments. Error bars represent mean ± SEM. BMDM cells were transfected with scrambled control (SC) or anti-PKM2 siRNA (D). 24 h post-transfection, cells were stimulated with LPS (100 ng/ml) for 24 h after which expression of PKM2 (top panel) and PDL1 (middle panel) was measured by western blot. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29081778), licensed under a CC-BY license. Not internally tested by R&D Systems.

AF1019) at 3ug/mL with one-hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lamina propria of villi. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of mouse intestines were probed for PD-L1 mRNA (ACD RNAScope Probe, catalog #420508; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse PD-L1 polyclonal antibody (R&D Systems catalog # <a class=

AF1019) at 3ug/mL with one-hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lamina propria of villi. " class="big_thumb" />

Protumor roles for TAMs and granulocytic myeloid-derived suppressor cells are obviated in neoantigen+ PDA.(A) Immunofluorescence staining of CB+ (nAg+) or CB– (nAg–) orthotopic tumors on day 21. Arrows show CD8+ T cell contact with a macrophage. Scale bar: 50 μm. Original magnification of insets, 2.25×. The number of CD8+ T cell and TAM (F4/80+) contacts per field of view. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35393950), licensed under a CC-BY license. Not internally tested by R&D Systems.

Representative images (×20 magnification) of H&E and PD-L1 IHC-stained sections (A-C): (A) Nude mouse lymph node showing normal histology (H&E) and diffuse staining for PD-L1 (mouse) with vessels and cells within the paracortical regions displaying increased staining intensity; (B) Nude mouse spleen showing normal histology (H&E) and intense membranous staining for PD-L1 (mouse) with the most intense staining at the periphery of the white pulp; (C) MDA-MB231 tumor showing a viable region (H&E necrotic regions were observed in all sections) with membranous and cytoplasmic staining for PD-L1 (human) on the tumor cells; (D) IHC quantitative analysis (staining intensity score, H-score) of PD-L1 expression levels in lymph nodes, spleen, and MDA-MB231 tumors from mouse xenografts; each bar represents the mean H-score ± standard deviation (SD; n = 3, spleen and lymph nodes; n= 6, tumors). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31044647), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human pancreas tissue from type 1 diabetic subjects express PD-L1. Human pancreas sections from type 1 diabetic (T1D), autoantibody positive (AA+), type 2 diabetic (T2D), and non-diabetic controls (NDB) were obtained from the Network for Pancreatic Organ Donation (nPOD) and stained for T cell markers (CD4, CD8), insulin, and PD-L1. Shown are representative islets from each group with 7–15 unique islets analyzed from three independent experiments with one patient per group. Scale bar corresponds to 20 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29844327), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1019U-100UG
Alexa Fluor 405	AF1019V-100UG
Alexa Fluor 488	AF1019G-100UG
Alexa Fluor 532	AF1019X-100UG
Alexa Fluor 594	AF1019T-100UG
Alexa Fluor 647	AF1019R-100UG
Alexa Fluor 700	AF1019N-100UG
Alexa Fluor 750	AF1019S-100UG
Alexa Fluor Plus 405	AF1019AFP405-100UG
Alexa Fluor Plus 488	AF1019AFP488-100UG
Alexa Fluor Plus 555	AF1019AFP555-100UG
Alexa Fluor Plus 594	AF1019AFP594-100UG
Alexa Fluor Plus 647	AF1019AFP647-100UG
Alexa Fluor Plus 680	AF1019AFP680-100UG
Allophycocyanin	FAB1019A-100
Allophycocyanin	FAB1019A-025
Biotin	BAF1019
CoraFluor 1	AF1019CL1
DyLight 350	AF1019D
DyLight 405	AF1019E
DyLight 488	AF1019K
DyLight 550	AF1019L
DyLight 594	AF1019M
DyLight 650	AF1019W
DyLight 680	AF1019Y
DyLight 755	AF1019Z
HRP	AF1019H
Janelia Fluor 525	AF1019JF525
Janelia Fluor 549	AF1019I
Janelia Fluor 646	AF1019J
Janelia Fluor 669	AF1019JF669
PE	AF1019P
PerCP	AF1019C
mFluor Violet 450 SE	AF1019MFV450
mFluor Violet 500 SE	AF1019MFV500
mFluor Violet 610 SE	AF1019MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1019-SP
Carrier Free	FAB1019A-100
Carrier Free	FAB1019A-025

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NBP1-76769

PD-L1 Antibody - BSA Free

(5 Reviews)

(42 Publications)

0.1 mg

NBP1-76769

PD-L1-Antibody-Immunohistochemistry-Paraffin-Negative-NBP1-76769-img0025.jpg

<b>Biological Strategies Validation. </b>PD-L1-Antibody-Western-Blot-NBP1-76769-img0027.jpg

Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP1-76769] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-76769 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: PD-L1 Antibody [NBP1-76769] - An intracellular stain was performed on U-251 MG cells with PD-L1 Antibody NBP1-76769 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

<b>Biological Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP1-76769] - Expression of CD274 in Human melanoma SK-MEL-28 cell line upon treatment with 200 mM ethanol. Dilution: 1:1,000 in PBS with 5% BSA. Secondary Ab: anti-Rabbit IgG 1:5,000. Western blot image submitted by a verified customer review.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Human tonsil tissue with PD-L1 antibody at 5 ug/mL.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - Staining of human heart tissue with antibody at 2.5 ug/mL.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - PD-L1/B7-H1 Antibody [NBP1-76769] - PD-L1 in rat heart tissue with at 5 ug/mL.

Flow Cytometry: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Analysis of A-20 cells using B7-H1/PD-L1/CD274 antibody at 0.5 ug/mL. Green: Isotype control. Red : B7-H1/PD-L1/CD274 antibody.

Immunofluorescence: PD-L1 Antibody [NBP1-76769] - Human Heart cells with PD-L1 antibody at 20 ug/mL.

Dual RNAscope ISH-IHC: PD-L1 Antibody [NBP1-76769] - PDCD1 mRNA (red) and PD-L1 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-PDCD1 and PD-L1 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Abrogation of autophagy by siRNAs targeting autophagy-related genes in gastric cancer cells induced tumor-intrinsic PD-L1 expression. a Inhibition of autophagy by knockdown of ATG5 in AGS & NCI-n87 gastric cancer cells induced the expression of PD-L1 in the presence & absence of INF-gamma (200 U/mL) as shown by flow cytometry analysis at 48 h post-transfection. b The induction of PD-L1 was confirmed by Western blots at 72 h post-transfection. The knockdown efficacies of ATG5 & ATG7 siRNA were verified. The conversion of LC3B-I to LC3B-II was reduced. Results were averaged & blots were representative of 4 independent experiments. The ratio of PD-L1 MFI minus isotype control was shown as mean ± S.D. relative to Ctrl from 4 independent experiments, *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30925913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of autophagy inhibitors in combination with IFN-gamma on expression of PD-L1 in gastric cancer cell lines. a The effect of chloroquine (CQ) or 3-MA on expression of PD-L1 with or without INF-gamma for 24 h was determined by flow cytometry assays. In AGS & NCI-n87 cells, MFI as the indication of PD-L1 expression level can be further increased by the treatment of INF-gamma . b Levels of LC3B-I/II, p62/SQSTM1 & PD-L1 were determined by Western blots in AGS & NCI-n87 cells treated by CQ, 3-MA, bafilomycin A1 (Baf) or rapamycin (Rap) for 24 h. c Positive staining of PD-L1 (red) & LC3 positive puncta (green) was determined by immunofluorescence in AGS & NCI-n87 cells treated by autophagy inhibitors & activator as in (b). d Rapamycin decreased the levels of PD-L1 protein in AGS & NCI-n87 cells as shown by flow cytometry. Results were averaged & blots were representative of 4 independent experiments, *p < 0.05, **p < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30925913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - ROS mediated PD-L1 upregulation in FFA-treated LO2 cells. (a) Intracellular ROS in LO2 cells before & after FFA treatment measured by DCFH-DA. (b–d) Expression levels of NOX2 & NOX4 measured using qRT-PCR & western blot. (e) JC-1 probes were used to detect mitochondrial membrane potential. (f–j) Intracellular ROS in LO2 cells pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment, measured by DCFH-DA, PD-L1 expression determined by western blot. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - Inhibition of NF-kappa B or STAT3 downregulated PD-L1 expression in adi-CM model. a PD-L1 protein levels in HepG2 cells treated with different concentration of NF-kappa B inhibitor, withaferin A (WA). b PD-L1 protein levels in HepG2 cells treated with different concentration of STAT3 inhibitor, BP-1-102. c PD-L1 protein levels in B16-F1 cells incubated with WA. Data are expressed as mean ± SEM, n = 6, **P < 0.01, ***P < 0.001 Vs adi-CM group. ##P < 0.01 & ###P < 0.001 Vs DMSO group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32477009), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - PD-L1 expression levels in liver samples & FFA-treated LO2 cells. (a–d) PD-L1 expression & CD8+ T cells in the liver tissues determined by immunohistochemistry. (e) Intracellular lipid accumulation after FFA treatment (0.8 mM) for 24 hour measured by Oil Red staining. (f–i) Expression levels of PD-L1 detected by qRT-PCR, western blot, & immunofluorescence. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - ROS/ZNF24/PD-L1 pathway activation in NAFLD models. (a) Intracellular ROS in hepatocytes in NAFLD significantly increased. (b) JC-1 probes were used to detect mitochondrial membrane potential. (c & d) NOX4 & ZNF24 expressions were determined by immunohistochemistry. (e & f) ROS/ZNF24/PD-L1 pathway activation examined by western blot. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - PD-L1 limits liver injury in NAFLD models. (a & b) Western blot determined the effect of siRNA against PD-L1. (c) After PD-L1 knockdown, mRNA expression levels of markers of T cell activation measured by qRT-PCR. (d & e) Hepatocyte injury was evaluated by the Tunel assay & ALT/AST measurement. (f) Graph illustrating that both ROS/ZNF24 pathway activation & UBE2I-mediated ZNF24 sumoylation suppression induced by FFA promoted PD-L1 expression. ∗P < 0.05, ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - PD-L1 knockdown aggravated the damage of CD8 + T cells to FFA-treated LO2 cells. (a & b) Western blot determined the effect of siRNA against PD-L1. Coculturing of FFA-treated LO2 cells & CD8 + T cells in vitro. (c) mRNA expression levels of markers of T cell activation measured by qRT-PCR, (d) LO2 cell injury was evaluated by LDH assay, (e) AST or ALT in the supernatants measured by commercial assay kits. # represents that CD8 + T cells were incubated separately with LO2 cells, but supernatants were put together. ∗P < 0.05, ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - Upregulated expression of PD‐L1 in cisplatin‐resistant cells through IL‐6/STAT3. The cisplatin resistant CAL27cis & Detroit‐562cis cells had increased IL‐6 mRNA & IL‐6 protein production compared to parental cells by qRT‐PCR & ELISA (A, B). CAL27cis & Detroit‐562cis were cultured with IL‐6 (10 ng/mL, human recombinant IL‐6, R&D Systems, Minneapolis, USA) for 48 h (C). An anti‐human IL‐6 polyclonal antibody (R&D Systems, Minneapolis, USA) was used to neutralize the biological activities of IL‐6 (E, F). To inhibit signal transduction, CAL27cis & Detroit‐562cis cells were pre‐incubated for 1 h with STAT3 inhibitor V (Sigma‐Aldrich, Germany, 500 nmol/L), & then incubated with recombinant human IL‐6 (10 ng/mL) for 24 h before analysis. Data are shown as mean ± SD of 3 independent triplicate cultures (A, B, D, E, F, H). *P < .05, **P < .01 & ***P < .001, compared with the results of control without IL‐6 (D), control IgG (E, F) & with IL‐6 alone (H) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29761938), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - ZNF24 promoted PD-L1 expression through binding to its promoter in FFA-treated LO2 cells. (a) A schematic of the target sites (wild & mutant) of ZNF24 in the promoter of PD-L1. (b–d) Dual-luciferase reporter assays performed in LO2 cells transfected with WT or MT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression. (e–g) ZNF24 expression after FFA treatment determined by qRT-PCR & western blot. (h & i) ZNF24 expression in LO2 cells pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment, measured by western blot. (j & k) ZNF24 & PD-L1 expression in LO2 cells pretreated with siRNA against ZNF24, followed by FFA treatment detected by western blot. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - LfcinB reduced IL‐6‐dependent PD‐L1 expression in cisplatin‐resistant HNSCC cells (A‐C) & inhibited the xenograft growth from CAL27cis & Detroit‐562cis (D, E). The markedly decreased IL‐6 (A) by ELISA & PD‐L1 by WB (B, C) in the acquired cisplatin‐resistant cells were detected. In figure A, C & D, data are shown as mean ± SD of 3 independent triplicate cultures. *P < .05 & **P < .01, compared with the results of control without IL‐6 (A, C). To investigate the effects of LfcinB on in vivo growth of CAL27cis & Detroit‐562cis, we treated nude mice carrying CAL27cis (D) & Detroit‐562cis (E) xenografts with once‐daily injection of 0.75 mg LfcinB (n = 5). Tumor growth of HNSCC xenografts displayed significant reduction after 3 days of LfcinB treatment, compared to control with saline (*P < .05 for LfcinB) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29761938), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - Identification of ZNF24–UBE2I protein interactions. (a & b) Analysis of protein interactions between ZNF24 & UBE2I using the BioGRID database, further confirmed by Co-IP assays. (c–f) UBE2I expression in LO2 cells treated by FFA or pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment measured by western blot. (g & h) After FFA treatment, Sumo-1: ZNF24 & ZNF24 expression levels detected by western blot. (i & j) After UBE2I overexpression, Sumo-1: ZNF24, ZNF24, PD-L1, & UBE2I expression levels were determined by western blot. (k–m) Dual-luciferase reporter assays performed in LO2 cells transfected with WT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression with or without Sumo-1 overexpression. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

PD-L1 was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-PD-L1 Antigen Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-76769AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76769AF350
Alexa Fluor 405	NBP1-76769AF405
Alexa Fluor 488	NBP1-76769AF488
Alexa Fluor 532	NBP1-76769AF532
Alexa Fluor 594	NBP1-76769AF594
Alexa Fluor 647	NBP1-76769AF647
Alexa Fluor 700	NBP1-76769AF700
Alexa Fluor 750	NBP1-76769AF750
Alexa Fluor Plus 405	NBP1-76769AFP405
Alexa Fluor Plus 488	NBP1-76769AFP488
Alexa Fluor Plus 555	NBP1-76769AFP555
Alexa Fluor Plus 594	NBP1-76769AFP594
Alexa Fluor Plus 647	NBP1-76769AFP647
Alexa Fluor Plus 680	NBP1-76769AFP680
Allophycocyanin	NBP1-76769APC
Biotin	NBP1-76769B
CoraFluor 1	NBP1-76769CL1
DyLight 350	NBP1-76769UV
DyLight 405	NBP1-76769V
DyLight 488	NBP1-76769G
DyLight 550	NBP1-76769R
DyLight 594	NBP1-76769DL594
DyLight 650	NBP1-76769C
DyLight 680	NBP1-76769FR
DyLight 755	NBP1-76769IR
FITC	NBP1-76769F
HRP	NBP1-76769H
Janelia Fluor 525	NBP1-76769JF525
Janelia Fluor 549	NBP1-76769JF549
Janelia Fluor 646	NBP1-76769JF646
Janelia Fluor 669	NBP1-76769JF669
PE	NBP1-76769PE
PerCP	NBP1-76769PCP
mFluor Violet 450 SE	NBP1-76769MFV450
mFluor Violet 500 SE	NBP1-76769MFV500
mFluor Violet 610 SE	NBP1-76769MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76769SS

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AF156

PD-L1 Antibody [Unconjugated]

(1 Review)

(26 Publications)

100 μg

AF156

HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

PD-L1/B7-H1 was detected in immersion fixed paraffin-embedded sections of normal human colon (left panel) and human colon cancer tissue (right panel) using Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB1561R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox">

Recombinant Human PD-L1/B7-H1 protein was serially diluted 2-fold and captured by Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # <a class=

MAB1561R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of HDLM-2 human Hodgkin's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PD-L1/B7-H1 at approximately 72 kDa (as indicated) using 50 µg/mL of Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

In vitro and ex vivo inhibition of PD-1/PD-L1 interaction.a PD-1/PD-L1 homogenous time-resolved fluorescence (HTRF) assay. b PD-L1/PD-L1 HTRF dimerization assay. c PD-1/PD-L1 NFAT reporter bioassay. d CMV recall assay: IFN gamma production measured by ELISA following stimulation of PBMCs with CMV antigens. Source data are provided as a Source data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33619272), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of compound A on ex vivo HBV-specific immune responses.a PBMCs stimulated with compound A for 1 h, followed by flow cytometric measurement of cell surface PD-L1 percentage. b PBMCs cultured in triplicate wells with HBV peptides for 10 days and T cell proliferation (thymidine incorporation) and IFN gamma secretion (ELISA) measured in the presence of compounds and antibodies. c PBMCs stimulated with compounds or antibodies overnight followed by IFN gamma producing cells measured by Fluorospot. d PBMCs collected from HBV vaccinated individuals (n = 3) on day 7 post booster vaccination were incubated with either rHBsAg alone (1 µg/ml) or rHBsAg plus compound A at 0.5 µM concentration for 5 days. Cells were then collected and stained for HBsAg-specific B cells or plasmablasts (PBs), using Atto488-labeled rHBsAg by flow cytometry. The error bars represent the standard error of mean of three individuals. statistical significance (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) was determined by unpaired two-tailed Student’s t test. Source data are provided as a Source data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33619272), licensed under a CC-BY license. Not internally tested by R&D Systems.

Modulation of soluble co-signaling molecules in kidney-transplanted patients over time.The levels of the soluble co-signaling molecules CD30, CD40, CD137, CD40L, PD-1 and PD-L1 were assayed by ELISA in serum samples of healthy controls (n = 25) and kidney-transplanted patients (n = 59) obtained at different times: just before transplantation, and 15 days, 3 months and 1 year after transplantation. Data are shown as box-plots, in which the horizontal line within each box represents the median, the bottom and top of each box represent the 25th and 75th percentiles, the bars represent the 10th and 90th percentiles and circles indicate outliers. Unpaired and paired Wilcoxon tests were used to compare distributions between independent and dependent groups, respectively. * indicates statistically significant differences between healthy controls and kidney-transplanted patient samples, and † indicates statistically significant differences between patients samples obtained at different pre- and post-transplantation times. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25478957), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Mechanism of action of compound A.a PD-L1 aAPC/CHO-K1 cells were treated with 0–500 nM compound A or compound B. PD-L1 protein visualized by native gel electrophoresis. b Native gel electrophoresis of PD-L1 protein following pre- and post-lysate treatment with compound A. c PD-L1 aAPC/CHO-K1 cell line was treated with compound A, alpha PD-1/ alpha PD-L1 antibodies, or no treatment followed by cell surface PD-L1 detected by flow cytometry. Values inside histogram represent GeoMean fluorescence intensity. d CHO-K1 cells transfected with cMyc-PD-L1 and labeled with anti-cMyc Alexa Fluor 488-conjugated antibody followed by incubation with compound A. The confocal fluorescence microscopy detects PD-L1-cMyc (green) and nucleus (blue). Images are representative of three experiments and a minimum of 50 cells observed in each experiment. e Whole-cell extracts of CHO-K1 cells co-transfected with cMyc-PD-L1 and Flag-PD-L1 were incubated with media + 0.5% DMSO, inactive (cmpd B) or active (cmpd A) compound, and subjected to immunoprecipitation (IP) with anti-cMyc magnetic beads and immunoblot with anti-Flag antibody. Blots are representative of three experiments. f PD-L1 aAPC/CHO-K1 cell line treated with compound A to allow for loss of cell surface PD-L1, followed by daily media washes. Black line represents diminishing concentration of compound A as measured by LC-MS. Red line represents reconstitution of cell surface PD-L1 as measured by flow cytometry of ≥10,000 cells per time point. g Compound A wash off and PD-L1 cell surface reconstitution in the presence of transcriptional inhibitor (actinomycin D) and protein transport inhibitor (golgi plug). Inactive compound B (gray line) referenced as negative control. Source data are provided as a Source data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33619272), licensed under a CC-BY license. Not internally tested by R&D Systems.

Chemotherapeutic drugs DOX and PTX increase WT1 levels in CAFs and enhance their ability to inhibit T cell proliferation. A Schematic overview of drug treatment, co-culture of CAFs and PBMCs followed by T cell proliferation assay. B Protein expression of WT1 and p53 in CAFs following DOX and PTX treatment was determined by Western blotting. GAPDH was used as a loading control. C Protein levels of STAT1, STAT3, PD-L1 levels. IDO release from DOX and PTX treated CAFs was determined by ELISA. D DOX treated CAFs were co-cultured with PBMCs and CFSE-labelled CD4+ and CD8+ T cell proliferation was determined by flow cytometry (E) PTX treated CAFs were co-cultured with PBMCs and CFSE-labelled CD4+ and CD8.+ T cell proliferation was determined by flow cytometry. Representative experiment is shown of n = 3 biological replicates. Data are mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40598415), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF156U-100UG
Alexa Fluor 405	AF156V-100UG
Alexa Fluor 488	AF156G-100UG
Alexa Fluor 532	AF156X-100UG
Alexa Fluor 594	AF156T-100UG
Alexa Fluor 647	AF156R-100UG
Alexa Fluor 700	AF156N-100UG
Alexa Fluor 750	AF156S-100UG
Alexa Fluor Plus 405	AF156AFP405-100UG
Alexa Fluor Plus 488	AF156AFP488-100UG
Alexa Fluor Plus 555	AF156AFP555-100UG
Alexa Fluor Plus 594	AF156AFP594-100UG
Alexa Fluor Plus 647	AF156AFP647-100UG
Alexa Fluor Plus 680	AF156AFP680-100UG
Allophycocyanin	AF156A
Biotin	BAF156
CoraFluor 1	AF156CL1
DyLight 350	AF156D
DyLight 405	AF156E
DyLight 488	AF156K
DyLight 550	AF156L
DyLight 594	AF156M
DyLight 650	AF156W
DyLight 680	AF156Y
DyLight 755	AF156Z
FITC	AF156F
HRP	AF156H
Janelia Fluor 525	AF156JF525
Janelia Fluor 549	AF156I
Janelia Fluor 646	AF156J
Janelia Fluor 669	AF156JF669
PE	AF156P
PerCP	AF156C
mFluor Violet 450 SE	AF156MFV450
mFluor Violet 500 SE	AF156MFV500
mFluor Violet 610 SE	AF156MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF156-SP

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156-B7

Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

(35 Publications)

100 μg

156-B7

G-102-C)." class="big_lightbox">

Recombinant Human PD-L1 / B7-H1 Fc Chimera (Catalog # 156-B7) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED<sub>50</sub> for this effect is 0.075-0.75 µg/mL in the presence of Goat Anti-Human IgG Fc Polyclonal Antibody (<a class=

G-102-C)." class="big_thumb" />

8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_lightbox">

Recombinant Human PD-L1/B7-H1 Fc protein (Catalog # 156-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (<a class=

8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	156-B7-01M

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MAB1561

PD-L1 Antibody (130021) [Unconjugated]

(24 Publications)

100 μg

MAB1561

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).View our protocol for Staining Membrane-associated Proteins." alt="Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). Adherent cells were prepared by either manual scraping or with TrypLE Express treatment with similar results. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

<b>Biological Strategies Validation. </b>MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). Adherent cells were prepared by either manual scraping or with TrypLE Express treatment with similar results. View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was observed in the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

PD-L1/B7-H1 was detected in formalin fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was observed in the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB1561) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of human colon cancer were probed for PDL1 mRNA (ACD RNAScope Probe, catalog # 600861; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human PDL1 monoclonal antibody (R&D Systems catalog # <a class=

MAB1561) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. " class="big_thumb" />

Chemotherapeutics induce PD-L1 expression in NPC cells and PD-1 expression in NK cells via upregulation of NF-kappa B. NPC cells a or NK cells c were incubated with the NF-kappa B inhibitor BMS-345541 for 1 h before incubation with chemotherapeutics. NPC cells were transfected with NF-kappa B siRNA b or NK cells with NF-kappa B siRNA d for 16 h and then incubated with chemotherapeutics. Expression of NF-kappa B, PD-L1 and PD-1 was analyzed by immunoblot Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32737537), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1561U-100UG
Alexa Fluor 405	FAB1561V-025
Alexa Fluor 488	FAB1561G-025
Alexa Fluor 488	FAB1561G-100
Alexa Fluor 532	FAB1561X
Alexa Fluor 594	FAB1561T-100UG
Alexa Fluor 647	FAB1561R-025
Alexa Fluor 700	FAB1561N-100
Alexa Fluor 700	FAB1561N-025
Alexa Fluor 750	FAB1561S-100UG
Alexa Fluor Plus 405	FAB1561AFP405-100UG
Alexa Fluor Plus 488	FAB1561AFP488-100UG
Alexa Fluor Plus 555	FAB1561AFP555-100UG
Alexa Fluor Plus 594	FAB1561AFP594-100UG
Alexa Fluor Plus 647	FAB1561AFP647-100UG
Alexa Fluor Plus 680	FAB1561AFP680-100UG
Allophycocyanin	FAB1561A-100
Allophycocyanin	FAB1561A-025
Allophycocyanin/Cy7	FAB1561APCCY7
Biotin	FAB1561B
CoraFluor 1	FAB1561CL1
DyLight 350	FAB1561D
DyLight 405	FAB1561E
DyLight 488	FAB1561K
DyLight 550	FAB1561L
DyLight 594	FAB1561M
DyLight 650	FAB1561W
DyLight 680	FAB1561Y
DyLight 755	FAB1561Z
FITC	FAB1561F
HRP	FAB1561H
Janelia Fluor 525	FAB1561JF525
Janelia Fluor 549	FAB1561I
Janelia Fluor 646	FAB1561J
Janelia Fluor 669	FAB1561JF669
PE/Atto594	FAB1561PEATT594
PE/Cy5.5	FAB1561PECY55
PE/Cy7	FAB1561PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB1561C-100
PerCP (Peridinin-chlorophyll Protein Complex)	FAB1561C-025
Phycoerythrin	FAB1561P-100
mFluor Violet 450 SE	FAB1561MFV450
mFluor Violet 500 SE	FAB1561MFV500
mFluor Violet 610 SE	FAB1561MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1561T-100UG
Carrier Free	MAB1561-SP
Carrier Free	FAB1561R-025
Carrier Free	FAB1561V-025
Carrier Free	FAB1561A-100
Carrier Free	FAB1561A-025
Carrier Free	FAB1561N-100
Carrier Free	FAB1561N-025
Carrier Free	FAB1561C-100
Carrier Free	FAB1561C-025
Carrier Free	FAB1561P-100
Carrier Free	FAB1561G-100
Carrier Free	FAB1561G-025
Carrier Free	FAB1561U-100UG
Carrier Free	FAB1561S-100UG

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1019-B7

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

(24 Publications)

100 μg

1019-B7

1 μg/lane of Recombinant Mouse PD-L1/B7-H1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 79 kDa and 150 kDa, respectively.

9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM. " class="big_lightbox">

Recombinant Mouse PD-L1/B7-H1 Fc protein (Catalog # 1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (<a class=

9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM. " class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP1-43262

PD-L1 Antibody (MIH5) - BSA Free

(1 Review)

(11 Publications)

0.1 mg

NBP1-43262

Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from verified customer review.

Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total PD-L1 status in the cancer cells. Image from verified customer review.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43262AF350
Alexa Fluor 405	NBP1-43262AF405
Alexa Fluor 488	NBP1-43262AF488
Alexa Fluor 532	NBP1-43262AF532
Alexa Fluor 594	NBP1-43262AF594
Alexa Fluor 647	NBP1-43262AF647
Alexa Fluor 700	NBP1-43262AF700
Alexa Fluor 750	NBP1-43262AF750
Alexa Fluor Plus 405	NBP1-43262AFP405
Alexa Fluor Plus 488	NBP1-43262AFP488
Alexa Fluor Plus 555	NBP1-43262AFP555
Alexa Fluor Plus 594	NBP1-43262AFP594
Alexa Fluor Plus 647	NBP1-43262AFP647
Alexa Fluor Plus 680	NBP1-43262AFP680
Allophycocyanin	NBP1-43262APC
Biotin	NBP1-43262B
CoraFluor 1	NBP1-43262CL1
DyLight 350	NBP1-43262UV
DyLight 405	NBP1-43262V
DyLight 488	NBP1-43262G
DyLight 550	NBP1-43262R
DyLight 594	NBP1-43262DL594
DyLight 650	NBP1-43262C
DyLight 680	NBP1-43262FR
DyLight 755	NBP1-43262IR
FITC	NBP1-43262F
HRP	NBP1-43262H
Janelia Fluor 525	NBP1-43262JF525
Janelia Fluor 549	NBP1-43262JF549
Janelia Fluor 646	NBP1-43262JF646
Janelia Fluor 669	NBP1-43262JF669
PE	NBP1-43262PE
PE/Cy5.5	NBP1-43262PECY55
PE/Cy7	NBP1-43262PECY7
PerCP	NBP1-43262PCP
mFluor Violet 450 SE	NBP1-43262MFV450
mFluor Violet 500 SE	NBP1-43262MFV500
mFluor Violet 610 SE	NBP1-43262MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43262-0.5mg
BSA Free	NBP1-43262-25ug

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DY156

Human PD-L1/B7-H1 DuoSet ELISA, 15 Plate

(9 Publications)

1 Kit (for 15 Plates)

DY156

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-15791

PD-L1 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP2-15791

<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

<b>Biological Strategies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody.

Western Blot: PD-L1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP2-15791] - MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Human ovarian cancer. PD-L1 stained by PD-L1 antibody diluted at 1:4000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

<b>Independent Antibodies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. NBP2-15791 on the left and competitor's antibody on the right.

Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 antibody diluted at 1:1000.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Detection of cell membranes in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody. <br></br>Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.

Western Blot: PD-L1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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9049-B7

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

(8 Publications)

100 μg

9049-B7

1086-PD) with an ED₅₀ of 0.05-0.3 µg/mL." class="big_lightbox">

When Recombinant Human PD-L1 / B7-H1 is immobilized at 0.025 µg/mL, it binds to Recombinant Human PD-1 Fc Chimera (Catalog # <a class=

1086-PD) with an ED₅₀ of 0.05-0.3 µg/mL." alt="When Recombinant Human PD-L1 / B7-H1 is immobilized at 0.025 µg/mL, it binds to Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) with an ED₅₀ of 0.05-0.3 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB90782

PD-L1 Antibody (2096A) - Azide and BSA Free

(1 Review)

100 μg

MAB90782

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	MAB90782AF350
Alexa Fluor 405	MAB90782AF405
Alexa Fluor 488	MAB90782AF488
Alexa Fluor 532	MAB90782AF532
Alexa Fluor 594	MAB90782AF594
Alexa Fluor 647	MAB90782AF647
Alexa Fluor 700	MAB90782AF700
Alexa Fluor 750	MAB90782AF750
Alexa Fluor Plus 405	MAB90782AFP405
Alexa Fluor Plus 488	MAB90782AFP488
Alexa Fluor Plus 555	MAB90782AFP555
Alexa Fluor Plus 594	MAB90782AFP594
Alexa Fluor Plus 647	MAB90782AFP647
Alexa Fluor Plus 680	MAB90782AFP680
Allophycocyanin	MAB90782APC
Allophycocyanin/Cy7	MAB90782APCCY7
Biotin	MAB90782B
DyLight 350	MAB90782UV
DyLight 405	MAB90782V
DyLight 488	MAB90782G
DyLight 550	MAB90782R
DyLight 594	MAB90782DL594
DyLight 650	MAB90782C
DyLight 680	MAB90782FR
DyLight 755	MAB90782IR
FITC	MAB90782F
HRP	MAB90782H
Janelia Fluor 549	MAB90782JF549
Janelia Fluor 646	MAB90782JF646
PE	MAB90782PE
PE/Atto594	MAB90782PEATT594
PE/Cy5.5	MAB90782PECY55
PE/Cy7	MAB90782PECY7
PerCP	MAB90782PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	MAB90782-SP

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MAB1019

PD-L1 Antibody (179711) [Unconjugated]

(1 Review)

(1 Publication)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1019U-100UG
Alexa Fluor 405	FAB1019V-100UG
Alexa Fluor 488	FAB1019G-100UG
Alexa Fluor 532	FAB1019X-100UG
Alexa Fluor 594	FAB1019T-100UG
Alexa Fluor 647	FAB1019R-100UG
Alexa Fluor 700	FAB1019N-100UG
Alexa Fluor 750	FAB1019S-100UG
Alexa Fluor Plus 405	FAB1019AFP405-100UG
Alexa Fluor Plus 488	FAB1019AFP488-100UG
Alexa Fluor Plus 555	FAB1019AFP555-100UG
Alexa Fluor Plus 594	FAB1019AFP594-100UG
Alexa Fluor Plus 647	FAB1019AFP647-100UG
Alexa Fluor Plus 680	FAB1019AFP680-100UG
Biotin	FAB1019B
CoraFluor 1	FAB1019CL1
DyLight 350	FAB1019D
DyLight 405	FAB1019E
DyLight 488	FAB1019K
DyLight 550	FAB1019L
DyLight 594	FAB1019M
DyLight 650	FAB1019W
DyLight 680	FAB1019Y
DyLight 755	FAB1019Z
HRP	FAB1019H
Janelia Fluor 525	FAB1019JF525
Janelia Fluor 549	FAB1019I
Janelia Fluor 646	FAB1019J
Janelia Fluor 669	FAB1019JF669
PE	FAB1019P
PerCP	FAB1019C
mFluor Violet 450 SE	FAB1019MFV450
mFluor Violet 500 SE	FAB1019MFV500
mFluor Violet 610 SE	FAB1019MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1019-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

PD-L1 Antibody [Unconjugated]

PD-L1 Antibody - BSA Free

PD-L1 Antibody [Unconjugated]

Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

PD-L1 Antibody (130021) [Unconjugated]

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

PD-L1 Antibody (MIH5) - BSA Free

Human PD-L1/B7-H1 DuoSet ELISA, 15 Plate

PD-L1 Antibody - BSA Free

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

PD-L1 Antibody (2096A) - Azide and BSA Free

PD-L1 Antibody (179711) [Unconjugated]