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VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei for epithelial cells in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF008). A specific band was detected for MDM2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MDM2 is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
![Immunohistochemistry MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-immunohistochemistry-9122020153128.jpg)
![ELISA MDM2/HDM2 [Biotin]](http://images.novusbio.com/fullsize/dyc1244-2_human-total-mdm2-hdm2-duoset-ic-elisa-2-plate-elisa-1092020101328.jpg)
![Immunohistochemistry MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MDM2_AF1244_Immunohistochemistry_7117.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MDM2_AF1244_Western_Blot_5099.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-121220259245222.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-121220259302814.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-121220259294514.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-121220259323717.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-121220259323714.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-121220259313321.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-12122025926921.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-121220259321517.jpg)
![Western Blot MDM2/HDM2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1244_human-mouse-rat-mdm2-hdm2-affinity-purified-polyclonal-ab-western-blot-12122025926917.jpg)
![N/A MDM2/HDM2 [Biotin]](http://images.novusbio.com/fullsize/dyc1244-2_human-total-mdm2-hdm2-duoset-ic-elisa-2-plate-17122020115113.png)
![N/A MDM2/HDM2 [Biotin]](http://images.novusbio.com/fullsize/dyc1244-2_human-total-mdm2-hdm2-duoset-ic-elisa-2-plate-17122020115216.png)
![Binding Activity MDM2/HDM2 [Unconjugated]](http://images.novusbio.com/images/11802-dm_recombinant-human-mdm2-hdm2-gst-tag-protein-cf-binding-activity-2652026104252.jpg)
![SDS-Page MDM2/HDM2 [Unconjugated]](http://images.novusbio.com/fullsize/11802-dm_recombinant-human-mdm2-hdm2-gst-tag-protein-cf-sds-page-2652026112349.jpg)
