| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87195-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44065 | |||
| BSA Free | NBP2-52878-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35686-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56537SS | |||
| Azide and BSA Free | NBP2-33221 | |||
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87195 (A) shows similar protein distribution across tissues to independent antibody NBP1-87196 (B).](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0022.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87195 (A) shows similar protein distribution across tissues to independent antibody NBP1-87196 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCM1 Antibody [NBP1-87195] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87195-img0008.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0013.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0014.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0015.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0016.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human liver.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0018.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human ovary.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0020.jpg)
![Flow Cytometry: PCM1 Antibody [NBP1-87195] - Cardiomyocyte-specific Cre-mediated recombination.(A) Representative flow cytometry plots depicting the gating strategy for sorting of cardiomyocyte nuclei. Positive 7-AAD (left panel) & PCM-1 (middle panel) staining identified cardiomyocyte nuclei. Purity of sorted cardiomyocyte nuclei is demonstrated in the right panel. FSC represents forward scatter. (B) Recombination rates in sorted male cardiomyocyte nuclei. Recombination rates were calculated from relative expression of respective Dnmt3 exons compared to Sry levels (n = 6). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0131019), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87195_rabbit-polyclonal-pcm1-antibody-310202415395961.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87196 (A) shows similar protein distribution across tissues to independent antibody NBP1-87195 (B).](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0023.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87196 (A) shows similar protein distribution across tissues to independent antibody NBP1-87195 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCM1 Antibody [NBP1-87196] - Staining of human cell line U-251 MG shows localization to nuclear membrane & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87196-img0018.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0015.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human ovary.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0021.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0022.jpg)
![Western Blot: PCM1 Antibody [NB100-58828] - Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-NB100-58828-img0004.jpg "Western Blot: PCM1 Antibody [NB100-58828] - Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PCM1 Antibody [NB100-58828] - Detection of Human and Mouse PCM1 on HeLa whole cell lysate using NB100-58828. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58829.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-NB100-58828-img0002.jpg)
![Western Blot: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-NB100-58828-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCM1 antibody NB100-58828 (lot NB100-58828-2) used for IP at 6 ug per reaction. PCM1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-58828-1) and rabbit anti-PCM1 antibody NB100-58829. For blotting immunoprecipitated PCM1, NB100-58828 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PCM1-Antibody-NB100-58828-img0005.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP2-52878] - Staining in human testis and pancreas tissues. Corresponding PCM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PCM1-Antibody-CL0206-Immunohistochemistry-Paraffin-NBP2-52878-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP2-52878] - Staining in human testis and pancreas tissues. Corresponding PCM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PCM1 Antibody (CL0206) [NBP2-52878] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/PCM1-Antibody-CL0206-Western-Blot-NBP2-52878-img0005.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP2-52878] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PCM1-Antibody-CL0206-Immunohistochemistry-Paraffin-NBP2-52878-img0007.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP2-52878] - Staining of human fallopian tube shows moderate to strong positivity in the apical cytoplasm of glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-CL0206-Immunohistochemistry-Paraffin-NBP2-52878-img0008.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP2-52878] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/PCM1-Antibody-CL0206-Immunohistochemistry-Paraffin-NBP2-52878-img0009.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP2-52878] - Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PCM1-Antibody-CL0206-Immunohistochemistry-Paraffin-NBP2-52878-img0010.jpg)
![Western Blot: PCM1 Antibody [NB100-58829] - Detection of Human PCM1 on HeLa whole cell lysate using NB100-58829. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58828.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-NB100-58829-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PCM1 Antibody [NB100-58829] - Detection of Human PCM1 on HeLa whole cell lysate using NB100-58829. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58828.")
![Immunocytochemistry/ Immunofluorescence: PCM1 Antibody [NBP3-35686] - Immunofluorescence analysis of U2OS cells using PCM1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35686_rabbit-pcm1-pab-912202414123899.jpg "Immunocytochemistry/ Immunofluorescence: PCM1 Antibody [NBP3-35686] - Immunofluorescence analysis of U2OS cells using PCM1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PCM1 Antibody [NBP3-35686] - Western blot analysis of lysates from Mouse brain, using PCM1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35686_rabbit-pcm1-pab-912202414213727.jpg)
![Western Blot: PCM1 Antibody [H00005108-B01P] - Analysis of PCM1 expression in transfected 293T cell line by PCM1 polyclonal antibody. Lane 1: PCM1 transfected lysate(58.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-H00005108-B01P-img0002.jpg "Western Blot: PCM1 Antibody [H00005108-B01P] - Analysis of PCM1 expression in transfected 293T cell line by PCM1 polyclonal antibody. Lane 1: PCM1 transfected lysate(58.3 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NB100-56537) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Knockdown-Validated-NB100-56537-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NB100-56537) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis using Azide and BSA Free version of NB100-56537. Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Western-Blot-NB100-56537-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - NIH3T3 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunocytochemistry-Immunofluorescence-NB100-56537-img0007.jpg)
![Immunohistochemistry-Paraffin: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of MBD1 (100B272.1) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and nuclear staining was observed.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunohistochemistry-Paraffin-NB100-56537-img0008.jpg)
![Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Western-Blot-NB100-56537-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunocytochemistry-Immunofluorescence-NB100-56537-img0006.jpg)