Product Search for parp: Novus Biologicals

AF835

Caspase-3 Antibody [Unconjugated] - Active

(7 Reviews)

50 μg

AF835

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for <a class=

Fluorescent ICC Staining of Cells on Coverslips." alt="Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # <a class=

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a higher magnification (I); the growth plate (J) and fibrocartilage of anterior cruciate ligament (K) show caspase-3 positive cells (red cytoplasmic staining; arrow). A negative control section is shown in (L). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/9/17729), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Immunohistochemical staining for caspase 3 on surgically transected tumours. A) Epo-treated tumour 24 hours after surgery. B) Placebo-treated tumour 24 hours after surgery. C) Placebo-treated tumour 48 hours after surgery Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-648), licensed under a CC-BY license. Not internally tested by R&D Systems.

LPS challenge induces cell shedding from small intestinal villi. C57 BL/6 mice were administered either (a) PBS (control) or (b) LPS by IP injection and proximal small intestines removed after 1.5 h. Processed tissue was sectioned and stained by immunohistochemistry for CC3 (i.e. brown cells indicate shedding event), also highlighted by arrows. A representative picture for each group is shown (12 mice per group, two independent experiments). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28123052), licensed under a CC-BY license. Not internally tested by R&D Systems.

The cytoprotective effect of B. breve is MyD88 dependent. (a,b) IEC MyD88+/+ mice and (c,d) IEC MyD88−/− mice were gavaged with PBS (control) or B. breve UCC2003 and challenged with LPS. Paraffin-embedded intestinal sections were stained with anti-CC3 and quantified using the WinCrypts and Score programs. (e) Columns shown TLR2 expression via RT-PCR. Data are mean ± s.d., n = 12 (two independent experiments) analysed with Mann–Whitney U-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28123052), licensed under a CC-BY license. Not internally tested by R&D Systems.

Bifidobacterium breve UCC2003 protects against LPS-induced cell shedding. C57 BL/6 mice received three daily oral gavage doses of (a) PBS or (b) approximately 1 × 109B. breve UCC2003 followed by IP challenge with LPS 24 h later. Representative images are shown. Formalin-fixed, paraffin-embedded intestinal sections were sectioned and stained with anti-CC3 and (c) quantified using the WinCrypts and Score programs, 20 well-orientated hemi-villi were counted per mouse. Data are mean ± s.d., n = 12 (two independent experiments) analysed with a Mann–Whitney U-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28123052), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Genetic deletion of Bax prevents mutant LRRK2 induced neuronal death. Primary embryonic cortical neurons are derived from WT mice or mice lacking the pro-apoptotic protein Bax, and transiently transfected with GFP-tagged WT or mutant LRRK2 (R1441C, G2019S). (a) A representative image showing typical morphological features of apoptotic neurons expressing mutant LRRK2; that are positive for active caspase-3 (casp3) and display condensed and fragmented nuclei. (b) Quantification of apoptotic neuronal death (apoptotic nuclear profiles, “apo”; and active caspase-3 positive neurons, “casp3”) from cultures depicted in (a); **p < 0.01 in comparison to WT transfected neurons. (c) Primary neurons were prepared from WT mice or mice deficient in Bax, and treated with the topoisomerase I inhibitor camptothecin (“cam”, 5 μM, 18 hr), or doxorubicin (“doxo”, 10 ng/ml, 36 hr). Cells were fixed and processed for apoptotic nuclear counts. (d) Primary cortical neurons were prepared from WT mice or mice deficient in Bax, and transiently transfected with WT, R1441C, or G2019S LRRK2. As before, the cultures were fixed and the percentage of LRRK2-positive neurons that displayed apoptotic nuclear features was determined. The absence of Bax significantly protected neurons from death induced by mutant LRRK2. ***p < 0.001 compared to WT neurons expressing R1441C- or G2019S-LRRK2. Each data point represents the mean +/− SEM from 3–4 independent transfections from a representative culture. Cultures were repeated at least 3 times with similar differences. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29472595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hepatocyte ARNT deletion alters fibrotic and apoptotic gene expression in the TAA model of fibrosis.Results for FC are shown in black columns and LARNT in white columns. Results are expressed relative to FC level. (A) Messenger RNA expression of ARNT and collagen isotypes. (B) Expression of genes regulating fibrosis in FC and LARNT mice. (C) F4/80 and inflammatory cytokine expression in FC and LARNT mice. (D) Apoptotic gene expression in FC and LARNT mice. (E) Representative histology of FC liver stained with anti-Caspase 3 at 100X magnification. (F) Representative histology of LARNT liver stained with anti-Caspase 3 at 100X magnification. (G) Average Caspase 3 positive cell counts per field of view at 100 X magnification. Mean±SEM, * = p<0.05 ** = p<0.01, *** = p<0.001. N = 5-6/group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121650), licensed under a CC-BY license. Not internally tested by R&D Systems.

p140Cap expression is causative in limiting tumour growth in NeuT mice.(a) Expression cassette used for the generation of MMTV-p140Cap transgenic mice. The Myc epitope is inserted at the carboxyterminal region of the protein. (b) Extracts of tumours derived from NeuT mice (a,b) or p140-NeuT mice (c,d) were run on 6% SDS–PAGE and stained with antibodies to p140Cap, ERBB2 and tubulin for loading control. (c) Percentage of tumour free mice in NeuT (red line) and p140-NeuT (blue-dashed line) transgenic animals in both FVB (left) or BALB/c background (right). Twelve mice were analysed for each group. Fisher's exact test, Two sided, P=0,0022; P=0,0056. Error bar: s.e.m. (d) Total tumour burden in NeuT (red) and p140-NeuT (blue) mice in both the FVB (left) or BALB/c backgrounds (right) was measured. Ten mice were analysed in each group. Unpaired t test: (*P<0.05; ***P<0.001). Error bar: s.e.m. (e) Paraffin-embedded sections from three NeuT and three p140-NeuT tumours taken from mice at 33 weeks of age were analysed for hematoxylin–eosin (H&E) (a–d) and for immunohistochemistry with antibodies to NeuT (e–h), PCNA (i–l) and activated Caspase3 (m–p). Representative images are shown. Scale bar, 50 μm (first and third columns); 20μm (second and fourth columns). Histograms on the right show the percentage of PCNA+ (upper panel) and Activated Caspase3+ (lower panel) cells. Statistical significative differences were evaluated using unpaired t-tests (***P<0.001). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14797), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Microglia-derived TNF-alpha alleviates aberrant neurogenesis.(a) qRT-PCR analyses of Tnf-alpha levels in the DG of WT and TLR9 KO mice at the indicated time points after seizure. Experimental controls were KA-untreated WT and TLR9 KO mice, respectively (n=3 animals). *P<0.05 by analysis of variance (ANOVA) with Tukey post-hoc tests. (b) Experimental scheme for assessing aNS/PC proliferation in microglia culture-derived CM pretreated with TNF-alpha -neutralizing antibody or IgG control. (c) Representative images of EdU (red), active caspase3 (green) and Hoechst (blue) staining in aNS/PCs cultured with the indicated CM from microglia (n=4 experiments). Scale bar, 50 μm. (d) Quantification of EdU+ or active caspase3+ cells in c (n=4 experiments). (e) Experimental timeline for assessing aNS/PC proliferation in thalidomide (Thal)-treated mice. (f) Representative images of BrdU+DCX+ newly generated immature neurons in the DG (n=4 animals). Scale bar, 50 μm. (g,h) Quantification of the number of BrdU+DCX+ cells in f, in the DG (g) and in the hilus (h) (n=4 animals). *P<0.05 and **P<0.01 by ANOVA with Tukey post-hoc tests. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25751136), licensed under a CC-BY license. Not internally tested by R&D Systems.

Double immunofluorescent staining of FSGS kidneys to Ki-67, alpha -tubulin and DAPI or double immunofluorescent staining to caspase-3 and DAPI. A. FSGS kidneys: cysts with simple squamous epithelium (CS) filled with colloid content, interstitium (i) and collecting tubules (ct) contain Ki-67-positive cells (arrows)(square). B. alpha -tubulin visualizes short cilia (arrows) in the cysts and extremely long cilia in collecting tubules (arrows)(square). C. Nuclear DAPI stain. D. Merging of A + B + C shows relationships between proliferating cells (green) and cilia (red). Immunostaining to Ki-67, alpha -tubulin and DAPI; scale bar 10 μm. E. Distribution of Ki-67 positive cells (%) in CNF and FSGS nephrotic syndrome. Cysts of proximal tubules with simple squamous epithelium (CS) or simple cuboidal epithelium (CC), proximal tubules with apparently normal simple cuboidal/columnar epithelium (PTNC). Data are shown as mean ± SD. Significant differences (Kruskal–Wallis) indicated by ***p < 0.0001. F. FSGS kidneys: caspase-3-positive apoptotic cells (arrows) in proximal tubules (PTNC) and interstitium (i). G. Nuclear DAPI stain. H. Merging of F + G shows green caspase-3- positive apoptotic nuclei and blue non-apoptotic nuclei. Immunostaining to caspase-3 and DAPI, scale bar 10 μm. Image collected and cropped by CiteAb from the following publication (//bmcnephrol.biomedcentral.com/articles/10.1186/1471-2369-15-3), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Epidermis-specific deletion of Rac1 increases UV-light-induced keratinocyte apoptosis in vivo. (a) H/E staining of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Black arrows indicate sunburn cells. (b) Graph shows the percentage number of sunburn cells at 12 h with (red bars) or without (blue bars) UV-irradiation in Rac1 fl/fl (n=4) and Rac1-EKO (n=5) mice. The percentage of sunburn cells within the epidermis after UV-irradiation in Rac1 fl/fl mice was 3.4%, whereas in Rac1-EKO mice it was 8.6% of total epidermal keratinocytes. Non-irradiated samples showed <1% sunburn cells in both the genotypes. (c) Immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Nuclei are stained in blue. Scale bar=100 μm. (d) Western blot analysis of cleaved caspase-3 from epidermal lysates of untreated (no UV) and UV-light treated (UV) Rac1 fl/fl and Rac1-EKO mice. Non-irradiated controls showed no bands for cleaved capsase-3, whereas samples of irradiated epidermis showed cleaved caspase-3-specific bands at ~25 kDa and 23 kDa. Numbers on the left denote molecular weights in kDa. (e) Graph shows densitometry analysis of western blot in (d). Error bars show S.D. Asterisks show P-value<0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

DNA damage has an important role in increased UV-light-induced keratinocyte apoptosis in Rac1-EKO epidermis in vivo. (a and c) H/E stainings and immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of CPDPL/Rac1-EKO mice kept in the dark and under the photoreactivation lamp. Nuclei are stained in blue. Dashed line in c demarcates border between epidermis and dermis. Scale bar=100 μm. (e) Western blot analysis of cleaved caspase-3 from epidermal lysates of UV-irradiated CPDPL/Rac1-EKO mice kept in the dark and under the photoreactivation lamp. GAPDH was used as a loading control. Numbers on the left denote molecular weight in kDa. (b,d, and f) Graphs show quantification of sunburn cells (n=5, 4) (b), cleaved caspase-3 positive cells (n=9, 8) (d) and densitometric analysis of cleaved caspase-3 western blots (n=4, 6) (f) from UV-irradiated CPDPL/Rac1-EKO mice kept in the dark (black bars) and under the photoreactivation (PR) lamp (gray bars). Error bars show S.D. Asterisks show P-value. **<0.01, ***<0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Smad1 deletion reduces oxidative stress and apoptosis following stroke.(A) Fluorescent images and quantification of in vivo HEt assay reflecting ROS levels on the ipsilateral hemisphere at 7 days post-stroke. The extent of oxidized nucleotides was measured by IHC for 8-oxo-dG levels at 24 h post-stroke. (B) IHC and quantification of activated Caspase 3 (aCasp3) and p53 on ipsilateral hemisphere at 7 days post-stroke. n = 3 for HEt, 8-oxo-dG, aCasp3, n = 4 for p53, unpaired Student’s t-test, *p<0.05; ***p<0.001. Scale, 50 μm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0136967), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on IL-1 beta -induced NF-kappa B-dependent expression of pro-inflammatory, pro-apoptotic and matrix degrading gene products, primary chondrocytes were either stimulated with 10 ng/ml IL-1 beta , 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) or pre-stimulated for 12 h with 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) followed by 10 ng/ml IL-1 beta for 24. Equal amounts of total proteins were separated by SDS-PAGE and analyzed by immunoblotting using antibodies raised against COX-2, MMP-9 and MMP-13 and active caspase-3. Stimulation with IL-1 beta resulted in production of COX-2, MMP-9, MMP-13 and caspase-3 cleavage. Pre-treatment with a combination of both IGF-1 or/and PDGF-bb downregulated COX-2, MMP-9, MMP-13 and cleaved caspase-3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0028663), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on IL-1 beta -induced NF-kappa B-dependent expression of pro-inflammatory, pro-apoptotic and matrix degrading gene products, primary chondrocytes were either stimulated with 10 ng/ml IL-1 beta , 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) or pre-stimulated for 12 h with 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) followed by 10 ng/ml IL-1 beta for 24. Equal amounts of total proteins were separated by SDS-PAGE and analyzed by immunoblotting using antibodies raised against COX-2, MMP-9 and MMP-13 and active caspase-3. Stimulation with IL-1 beta resulted in production of COX-2, MMP-9, MMP-13 and caspase-3 cleavage. Pre-treatment with a combination of both IGF-1 or/and PDGF-bb downregulated COX-2, MMP-9, MMP-13 and cleaved caspase-3. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22194879), licensed under a CC-BY license. Not internally tested by R&D Systems.

Allelic loss of Cdh11 led to faster growing tumors due to decreased cell death. (E) Cadherin-11 was knocked down using stealth siRNA in a cadherin-11 expressing cell line derived from TAg-RB tumors, T +539. Knockdown of Cdh11 clearly decreased caspase-3 expression compared to control. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20421947), licensed under a CC-BY license. Not internally tested by R&D Systems.

Curcumin inhibits IL-1 beta activity, enabling co-culture induced chondrogenesis in MSCs. A: Fourteen days high-density culture. Untreated MSC cultures became apoptotic (a). In primary chondrocyte cultures (b), co-cultures (c), co-cultures treated with curcumin (e) or co-cultures pre-stimulated four hours with curcumin (f), prominent chondrogenesis was observed. Stimulation of the co-culture with IL-1 beta alone resulted in degeneration of the cell culture (d). In contrast, a four-hour pre-stimulation of the co-culture with curcumin followed by IL-1 beta incubation (g) or a four-hour pre-stimulation of the co-culture with curcumin followed by IL-1 beta and curcumin incubation (h) inhibited the adverse effects of IL-1 beta on the chondrogenic potential of the co-culture and prominent chondrogenesis was observed. Magnification, 6,000×; bar, 1 μm; C, chondrocytes, F, fibroblast-like cells; M, ECM. B-D: Immunoblots of whole cell lysates were probed with antibodies against CSPGs, collagen type II, beta 1-integrin, Shc, activated-ERK1/2, Sox-9, activated-caspase-3 and COX-2. In co-cultures pre-stimulated for four hours with curcumin followed either by incubation with IL-1 beta alone or incubation with IL-1 beta and curcumin, prominent production of chondrogenic matrix and adhesion molecules (B), activation of the chondrogenic signalling pathway (C) and down-regulation of apoptotic and inflammatory markers (D) was observed. Each experiment was performed in triplicate. Expression of the housekeeping gene beta -actin was not affected. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20594343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF835-SP

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NB100-56599

PARP Antibody (194C1439) - Cleaved - BSA Free

(10 Publications)

0.1 mg

NB100-56599

Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.

Reactivity:

Hu, Mu, Rt(-)

Species Glossary

Reactivity: Hu, Mu, Rt(-)

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-401A

Alt Catalog# IMG-401A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27335AF350
Alexa Fluor 405	NBP2-27335AF405
Alexa Fluor 488	NBP2-27335AF488
Alexa Fluor 532	NBP2-27335AF532
Alexa Fluor 594	NBP2-27335AF594
Alexa Fluor 647	NBP2-27335AF647
Alexa Fluor 700	NBP2-27335AF700
Alexa Fluor 750	NBP2-27335AF750
Alexa Fluor Plus 405	NBP2-27335AFP405
Alexa Fluor Plus 488	NBP2-27335AFP488
Alexa Fluor Plus 555	NBP2-27335AFP555
Alexa Fluor Plus 594	NBP2-27335AFP594
Alexa Fluor Plus 647	NBP2-27335AFP647
Alexa Fluor Plus 680	NBP2-27335AFP680
Allophycocyanin	NBP2-27335APC
Biotin	NBP2-27335B
CoraFluor 1	NBP2-27335CL1
DyLight 350	NBP2-27335UV
DyLight 405	NBP2-27335V
DyLight 488	NBP2-27335G
DyLight 550	NBP2-27335R
DyLight 594	NBP2-27335DL594
DyLight 650	NBP2-27335C
DyLight 680	NBP2-27335FR
DyLight 755	NBP2-27335IR
FITC	NBP2-27335F
HRP	NBP2-27335H
Janelia Fluor 525	NBP2-27335JF525
Janelia Fluor 549	NBP2-27335JF549
Janelia Fluor 646	NBP2-27335JF646
Janelia Fluor 669	NBP2-27335JF669
PE	NBP2-27335PE
PerCP	NBP2-27335PCP
mFluor Violet 450 SE	NBP2-27335MFV450
mFluor Violet 500 SE	NBP2-27335MFV500
mFluor Violet 610 SE	NBP2-27335MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-27335
BSA Free	NB100-56599SS
Azide and BSA Free	NBP2-27335SS

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NBP2-13732

PARP Antibody - BSA Free

(1 Review)

(4 Publications)

0.1 ml

NBP2-13732

Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.

Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human pancreas shows strong nuclear positivity.

Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human parathyroid gland shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-13732-25ul

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AF-600-NA

PARP Antibody [Unconjugated]

(7 Publications)

100 μg

AF-600-NA

HAF109). A specific band was detected for PARP at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 ng/mL anti-Fas for 24 hours. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Human/Mouse PARP Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for PARP at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 ng/mL anti-Fas for 24 hours. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Human/Mouse PARP Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PARP at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>PARP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PARP at approximately 122 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

recommended buffer recipes for immunoprecipitation." class="big_lightbox">

<b>Biological Strategies Validation. </b>Jurkat human acute T cell leukemia cell line was treated with apoptosis inducer anti-Fas for the indicated times. PARP was immunoprecipitated from cell lysates (1 - 2 x 10<SUP>6</SUP>cells) following incubation with 5 µg Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) for overnight at 4 °C. PARP-antibody complexes were absorbed using Protein G expressing Staph cells (Sigma). Immunoprecipitated PARP was detected by Western blot using 0.4 µg/mL Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA). View our <a class=NoLineLink href=

recommended buffer recipes for immunoprecipitation." class="big_thumb" />

HAF017). A specific band was detected for PARP at approximately 120 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293Tcell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HEK293T human embryonic kidney parental cell line and PARP knockout HEK293T cell line (KO). PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for PARP at approximately 120 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293Tcell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-600-NAU-100UG
Alexa Fluor 405	AF-600-NAV-100UG
Alexa Fluor 488	AF-600-NAG-100UG
Alexa Fluor 532	AF-600-NAX-100UG
Alexa Fluor 594	AF-600-NAT-100UG
Alexa Fluor 647	AF-600-NAR-100UG
Alexa Fluor 700	AF-600-NAN-100UG
Alexa Fluor 750	AF-600-NAS-100UG
Alexa Fluor Plus 405	AF-600-NAAFP405
Alexa Fluor Plus 488	AF-600-NAAFP488
Alexa Fluor Plus 555	AF-600-NAAFP555
Alexa Fluor Plus 594	AF-600-NAAFP594
Alexa Fluor Plus 647	AF-600-NAAFP647
Alexa Fluor Plus 680	AF-600-NAAFP680
Allophycocyanin	AF-600-NAA
Biotin	BAF600
CoraFluor 1	AF-600-NACL1
DyLight 350	AF-600-NAD
DyLight 405	AF-600-NAE
DyLight 488	AF-600-NAK
DyLight 550	AF-600-NAL
DyLight 594	AF-600-NAM
DyLight 650	AF-600-NAW
DyLight 680	AF-600-NAY
DyLight 755	AF-600-NAZ
FITC	AF-600-NAF
HRP	AF-600-NAH
Janelia Fluor 525	AF-600-NAJF525
Janelia Fluor 549	AF-600-NAI
Janelia Fluor 646	AF-600-NAJ
Janelia Fluor 669	AF-600-NAJF669
PE	AF-600-NAP
PerCP	AF-600-NAC
mFluor Violet 450 SE	AF-600-NAMFV450
mFluor Violet 500 SE	AF-600-NAMFV500
mFluor Violet 610 SE	AF-600-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-600-SP

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MAB8095

PARP Antibody (839120) [Unconjugated]

(3 Publications)

100 μg

MAB8095

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PARP Monoclonal Antibody (Catalog # MAB8095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PARP at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PARP was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human PARP Monoclonal Antibody (Catalog # MAB8095) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for PARP at approximately 125 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PARP Monoclonal Antibody (Catalog # MAB8095). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB8095U-100UG
Alexa Fluor 405	FAB8095V-100UG
Alexa Fluor 488	FAB8095G-100UG
Alexa Fluor 532	FAB8095X-100UG
Alexa Fluor 594	FAB8095T-100UG
Alexa Fluor 647	FAB8095R-100UG
Alexa Fluor 700	FAB8095N-100UG
Alexa Fluor 750	FAB8095S-100UG
Alexa Fluor Plus 405	FAB8095AFP405-100UG
Alexa Fluor Plus 488	FAB8095AFP488-100UG
Alexa Fluor Plus 555	FAB8095AFP555-100UG
Alexa Fluor Plus 594	FAB8095AFP594-100UG
Alexa Fluor Plus 647	FAB8095AFP647-100UG
Alexa Fluor Plus 680	FAB8095AFP680-100UG
Allophycocyanin	FAB8095A
Biotin	FAB8095B
CoraFluor 1	FAB8095CL1
DyLight 350	FAB8095D
DyLight 405	FAB8095E
DyLight 488	FAB8095K
DyLight 550	FAB8095L
DyLight 594	FAB8095M
DyLight 650	FAB8095W
DyLight 680	FAB8095Y
DyLight 755	FAB8095Z
FITC	FAB8095F
HRP	FAB8095H
Janelia Fluor 525	FAB8095JF525
Janelia Fluor 549	FAB8095I
Janelia Fluor 646	FAB8095J
Janelia Fluor 669	FAB8095JF669
PE	FAB8095P
PerCP	FAB8095C
mFluor Violet 450 SE	FAB8095MFV450
mFluor Violet 500 SE	FAB8095MFV500
mFluor Violet 610 SE	FAB8095MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB8095-SP

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NBP2-36747

PARP Antibody (CL2220) - BSA Free

0.1 ml

NBP2-36747

Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human tonsil shows strong nuclear positivity in lymphoid cells.

Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.

Staining in HeLa cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in A431 cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in MCF7 cell line with Anti-PARP1 monoclonal antibody, showing clear cycle dependent nuclear staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U2OS cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U251 cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44461AF350
Alexa Fluor 405	NBP3-44461AF405
Alexa Fluor 488	NBP3-44461AF488
Alexa Fluor 532	NBP3-44461AF532
Alexa Fluor 594	NBP3-44461AF594
Alexa Fluor 647	NBP3-44461AF647
Alexa Fluor 700	NBP3-44461AF700
Alexa Fluor 750	NBP3-44461AF750
Alexa Fluor Plus 405	NBP3-44461AFP405
Alexa Fluor Plus 488	NBP3-44461AFP488
Alexa Fluor Plus 555	NBP3-44461AFP555
Alexa Fluor Plus 594	NBP3-44461AFP594
Alexa Fluor Plus 647	NBP3-44461AFP647
Alexa Fluor Plus 680	NBP3-44461AFP680
Allophycocyanin	NBP3-44461APC
Biotin	NBP3-44461B
CoraFluor 1	NBP3-44461CL1
DyLight 350	NBP3-44461UV
DyLight 405	NBP3-44461V
DyLight 488	NBP3-44461G
DyLight 550	NBP3-44461R
DyLight 594	NBP3-44461DL594
DyLight 650	NBP3-44461C
DyLight 680	NBP3-44461FR
DyLight 755	NBP3-44461IR
FITC	NBP3-44461F
HRP	NBP3-44461H
Janelia Fluor 525	NBP3-44461JF525
Janelia Fluor 549	NBP3-44461JF549
Janelia Fluor 646	NBP3-44461JF646
Janelia Fluor 669	NBP3-44461JF669
PE	NBP3-44461PE
PerCP	NBP3-44461PCP
mFluor Violet 450 SE	NBP3-44461MFV450
mFluor Violet 500 SE	NBP3-44461MFV500
mFluor Violet 610 SE	NBP3-44461MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44461
BSA Free	NBP2-36747-25ul

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NBP2-36748

PARP Antibody (CL2209) - BSA Free

0.1 ml

NBP2-36748

Western Blot: PARP Antibody (CL2209) [NBP2-36748] - Lane 1: Marker [kDa]. Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human skin shows strong nuclear immunoreactivity in both epidermis and dermis.

Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human cerebellum shows strong nuclear positivity in granular and molecular layer cells, as well as in Purkinje cells.

Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human kidney shows nuclear positivity in both tubules and glomeruli.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44462AF350
Alexa Fluor 405	NBP3-44462AF405
Alexa Fluor 488	NBP3-44462AF488
Alexa Fluor 532	NBP3-44462AF532
Alexa Fluor 594	NBP3-44462AF594
Alexa Fluor 647	NBP3-44462AF647
Alexa Fluor 700	NBP3-44462AF700
Alexa Fluor 750	NBP3-44462AF750
Alexa Fluor Plus 405	NBP3-44462AFP405
Alexa Fluor Plus 488	NBP3-44462AFP488
Alexa Fluor Plus 555	NBP3-44462AFP555
Alexa Fluor Plus 594	NBP3-44462AFP594
Alexa Fluor Plus 647	NBP3-44462AFP647
Alexa Fluor Plus 680	NBP3-44462AFP680
Allophycocyanin	NBP3-44462APC
Biotin	NBP3-44462B
CoraFluor 1	NBP3-44462CL1
DyLight 350	NBP3-44462UV
DyLight 405	NBP3-44462V
DyLight 488	NBP3-44462G
DyLight 550	NBP3-44462R
DyLight 594	NBP3-44462DL594
DyLight 650	NBP3-44462C
DyLight 680	NBP3-44462FR
DyLight 755	NBP3-44462IR
FITC	NBP3-44462F
HRP	NBP3-44462H
Janelia Fluor 525	NBP3-44462JF525
Janelia Fluor 549	NBP3-44462JF549
Janelia Fluor 646	NBP3-44462JF646
Janelia Fluor 669	NBP3-44462JF669
PE	NBP3-44462PE
PerCP	NBP3-44462PCP
mFluor Violet 450 SE	NBP3-44462MFV450
mFluor Violet 500 SE	NBP3-44462MFV500
mFluor Violet 610 SE	NBP3-44462MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44462
BSA Free	NBP2-36748-25ul

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NBP3-15802

PARP Antibody (2G8N7)

100 ul

NBP3-15802

Immunocytochemistry/ Immunofluorescence: PARP Antibody (2G8N7) [PARP] - Immunofluorescence analysis of paraffin-embedded human lung using PARP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: PARP Antibody (2G8N7) [PARP] - Immunofluorescence analysis of HeLa cells using PARP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human spleen using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human colon using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Rat heart using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Rat liver using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-09070

PARP Antibody

100 ul

NBP3-09070

Flow Cytometry: PARP Antibody [NBP3-09070] - Flow cytometric analysis of K562 cells with PARP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-09070] - ICC staining PARP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-26439

PARP Antibody (8C7) - BSA Free

100 ul

NBP3-26439

Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 KDa Observed band size: 114 kDa

Immunocytochemistry/Immunofluorescence: PARP Antibody (8C7) [NBP3-26439] - Staining of Hela Cells with PARP Antibody (8C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: PARP Antibody (8C7) [NBP3-26439] - Overlay histogram showing Jurkat cells stained with PARP Antibody (8C7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-26439-50ul

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NBP3-26438

PARP Antibody (7C11) - BSA Free

100 ul

NBP3-26438

Western Blot: PARP Antibody (7C11) [NBP3-26438] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-26438-50ul

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NBP3-15813

PARP Antibody (4M9X4) - Cleaved

100 ul

NBP3-15813

<b>Biological Strategies Validation. </b>Immunoprecipitation: PARP Antibody (4M9X4) - Cleaved [PARP] - Immunoprecipitation analysis of 600 ug extracts of Jurkat cells using 3 ug PARP antibody . Western blot was performed from the immunoprecipitate using PARP antibody at a dilution of 1:500.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Caspase-3 Antibody [Unconjugated] - Active

PARP Antibody (194C1439) - Cleaved - BSA Free

PARP Antibody - BSA Free

PARP Antibody [Unconjugated]

PARP Antibody (839120) [Unconjugated]

PARP Antibody (CL2220) - BSA Free

PARP Antibody (CL2209) - BSA Free

PARP Antibody (2G8N7)

PARP Antibody

PARP Antibody (8C7) - BSA Free

PARP Antibody (7C11) - BSA Free

PARP Antibody (4M9X4) - Cleaved