| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98769-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98768-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24697SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26418-50ul | |||
![Western Blot: Histone H3.3A Antibody [NBP2-98769] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP2-98769-img0002.jpg "Western Blot: Histone H3.3A Antibody [NBP2-98769] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769] - Histone H3.3A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate 2 ul anti-Histone H3.3A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Histone H3.3A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Immunoprecipitation-NBP2-98769-img0001.jpg)
![Western Blot: Histone H3.3A Antibody [NBP2-84066] - Host: Rabbit. Target Name: H3F3A. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP2-84066-img0001.jpg "Western Blot: Histone H3.3A Antibody [NBP2-84066] - Host: Rabbit. Target Name: H3F3A. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: Histone H3.3A Overexpression Lysate (Adult Normal) [NBL1-11428] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H3.3A.](http://images.novusbio.com/fullsize/Histone-H3-3A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11428-img0002.jpg "Western Blot: Histone H3.3A Overexpression Lysate (Adult Normal) [NBL1-11428] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H3.3A.")
![Western Blot: Histone H3.3A Antibody [NBP2-98768] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: C6 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP2-98768-img0001.jpg "Western Blot: Histone H3.3A Antibody [NBP2-98768] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: C6 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa")
![SDS-Page: Histone H3.3A Protein [NBP2-23098]](http://images.novusbio.com/fullsize/Histone-H3.3A-Protein-SDS-Page-NBP2-23098-img0001.jpg "SDS-Page: Histone H3.3A Protein [NBP2-23098]")
![Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Western-Blot-NBP2-24697-img0003.jpg "Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697] - Human small intestine stained with Histone H3.3 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Immunohistochemistry-Paraffin-NBP2-24697-img0002.jpg)
![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0008.jpg "Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0014.jpg)
![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human lung tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0011.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human cervix tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0012.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0013.jpg)
![Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of various cell lines, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Western-Blot-NBP3-16489-img0005.jpg "Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of various cell lines, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of Rat lung, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Western-Blot-NBP3-16489-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Immunohistochemistry of paraffin-embedded rat kidney using Histone H3.3 Rabbit mAb (NBP3-16489) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Immunohistochemistry-Paraffin-NBP3-16489-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Immunohistochemistry of paraffin-embedded human esophageal using Histone H3.3 Rabbit mAb (NBP3-16489) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Immunohistochemistry-Paraffin-NBP3-16489-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H3.3 Antibody (5C5B8) [Histone H3.3] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3.3 Rabbit mAb . The ChIP sequencing results indicate the enrichment pattern of Histone H3.3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.](http://images.novusbio.com/fullsize/nbp3-16489_rabbit-histone-h3-3-mab-5c5b8-5220251718458.jpg)
![Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202411472125.jpg "Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Analysis of Histone H3.3 [p Thr3] Antibody (28H4) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-28220241323624.jpg)
![Flow Cytometry: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Overlay histogram showing Hela cells stained with Histone H3.3 [p Thr3] Antibody (28H4) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202414183663.jpg)