HAF018). A specific band was detected for CD3 delta/CD3d at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CD3 delta/CD3d at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 delta/CD3d Monoclonal Antibody (Catalog # MAB10672) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 delta/CD3d at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

9850-CD) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL." class="big_lightbox"> 9850-CD) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL." alt="When Recombinant Human CD3 epsilon Fc Chimera Protein (9850-CD) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL." class="big_thumb" />