| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-63062-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-2005-0.002g | |||
| HRP | NB600-850-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14114 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14115 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72557 | |||
![Immunocytochemistry/Immunofluorescence: Lysozyme Antibody [NBP2-61118] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme NBP2-61118 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61118-img0006.jpg "Immunocytochemistry/Immunofluorescence: Lysozyme Antibody [NBP2-61118] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme NBP2-61118 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Lysozyme Antibody [NBP2-61118] - Total protein from human cell lines THP-1 and HepG2, human stomach and kidney as well as mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Lysozyme in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Western-Blot-NBP2-61118-img0003.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-61118] - Analysis of a FFPE tissue section of human kidney with Lysozyme antibody at 1:100 dilution. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Lysozyme antibody generated an expected cytoplasmic staining in all the cells with strongest signal in tubular epithelial cells. Some tubules showed more of a punctate staining pattern (vesicular Lysozyme) while all of the tubules depicted a diffused signal (secreted Lysozyme). A subset of cells, especially those from glomeruli, showed nuclear positivity also.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-61118-img0001.jpg)
![Flow Cytometry: Lysozyme Antibody [NBP2-61118] - An intracellular stain was performed on HepG2 cells with NBP2-61118 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Flow-Cytometry-NBP2-61118-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Lysozyme Antibody [NBP2-61118] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61118-img0002.jpg)
![Flow Cytometry: Lysozyme Antibody [NBP2-61118] - An intracellular stain was performed on THP-1 Cells with NBP2-61118 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Flow-Cytometry-NBP2-61118-img0005.jpg)
![Simple Western: Lysozyme Antibody [NBP2-61118] - Lane view shows lysates of Ileum stem cells undifferentiated and day 5 differentiated, loaded at 0.2 mg/mL. A specific band was detected for Lysozyme at approximately 20 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Lysozyme Polyclonal Antibody (NBP2-61118). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Simple-Western-NBP2-61118-img0004.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Analysis in human stomach and skeletal muscle tissues. Corresponding Lysozyme RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Analysis in human stomach and skeletal muscle tissues. Corresponding Lysozyme RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lysozyme Antibody [NBP2-33518] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Western-Blot-NBP2-33518-img0005.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human stomach shows moderate extracellular positivity in glandular cells.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunohistochemistry-NBP2-33518-img0016.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human kidney shows strong extracellular positivity in cells in tubules.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0013.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human lymph node shows moderate extracellular positivity in non-germinal center cells.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0014.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0015.jpg)
![CyTOF-ready: Lysozyme Antibody (BGN/0696/5B1) [NB100-63062] - Human bone marrow after RBC lysis was stained with surface antibodies, fixed, permeabilized with methanol, stained with anti-lysozyme NB100-63062, and gated for the above populations per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(BGN-0696-5B1)-CyTOF-ready-NB100-63062-img0001.jpg "CyTOF-ready: Lysozyme Antibody (BGN/0696/5B1) [NB100-63062] - Human bone marrow after RBC lysis was stained with surface antibodies, fixed, permeabilized with methanol, stained with anti-lysozyme NB100-63062, and gated for the above populations per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0010.jpg "Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody.Lane 1: Lysozyme (Hen Egg White).Load: 50 ng per lane.Primary antibody: Lysozyme antibody at 1:1,000 overnight at 4C.Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0009.jpg)
![ELISA: Lysozyme Antibody [NBP1-77790] - ELISA Results of Lysozyme antibody. Each well was coated in duplicate with 1.0 ug of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-ELISA-NBP1-77790-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Lysozyme Antibody (LZ598-10G9) [NBP2-62223] - Staining of lysozyme (green; A, C) in Hep G2 cells (A, B, C) using mouse monoclonal antibody. Actin decorated with phalloidin (red), DNA stained with DAPI (blue). Figure D represents SK-BR-3 cells with no expression of lysozyme, stained with antibody (no background signal).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LZ598-10G9)-Immunocytochemistry-Immunofluorescence-NBP2-62223-img0005.jpg "Immunocytochemistry/Immunofluorescence: Lysozyme Antibody (LZ598-10G9) [NBP2-62223] - Staining of lysozyme (green; A, C) in Hep G2 cells (A, B, C) using mouse monoclonal antibody. Actin decorated with phalloidin (red), DNA stained with DAPI (blue). Figure D represents SK-BR-3 cells with no expression of lysozyme, stained with antibody (no background signal).")
![Flow (Intracellular): Lysozyme Antibody (LZ598-10G9) [NBP2-62223] - Intracellular staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human lysozyme (LZ598-10G9) Gd155. Gated on native.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LZ598-10G9)-Flow-(Intracellular)-NBP2-62223-img0004.jpg)
![Flow (Intracellular): Lysozyme Antibody (LZ598-10G9) [NBP2-62223] - Intracellular staining of lysozyme in human peripheral blood with anti-lysozyme (LZ598-10G9) FITC.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LZ598-10G9)-Flow-(Intracellular)-NBP2-62223-img0001.jpg)
![Flow (Intracellular): Lysozyme Antibody (LZ598-10G9) [NBP2-62223] - Intracellular staining of lysozyme in human peripheral blood with anti-lysozyme (LZ598-10G9) PE.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LZ598-10G9)-Flow-(Intracellular)-NBP2-62223-img0002.jpg)
![Flow (Intracellular): Lysozyme Antibody (LZ598-10G9) [NBP2-62223] - Intracellular staining of lysozyme in human peripheral blood with anti-lysozyme (LZ598-10G9) purified, GAM-FITC.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LZ598-10G9)-Flow-(Intracellular)-NBP2-62223-img0003.jpg)
![Western Blot: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Western blot analysis of human spleen tissue and HepG2 cell lysates using Lysozyme Antibody (LYZ/3942).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LYZ-3942)-Western-Blot-NBP3-13961-img0004.jpg "Western Blot: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Western blot analysis of human spleen tissue and HepG2 cell lysates using Lysozyme Antibody (LYZ/3942).")
![Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Formalin-fixed, paraffin-embedded human tonsil stained with Lysozyme antibody (LYZ/3942).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LYZ-3942)-Immunohistochemistry-Paraffin-NBP3-13961-img0002.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Formalin-fixed, paraffin-embedded human spleen stained with Lysozyme antibody (LYZ/3942). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LYZ-3942)-Immunohistochemistry-Paraffin-NBP3-13961-img0001.jpg)
![Protein Array: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Lysozyme Antibody (LYZ/3942).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LYZ-3942)-Protein-Array-NBP3-13961-img0005.jpg)
![Western Blot: Lysozyme Antibody (ARQ4974) [NBP3-15338] - Western blot analysis of extracts of various cell lines, using Lysozyme antibody (NBP3-15338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(ARQ4974)-Western-Blot-NBP3-15338-img0002.jpg "Western Blot: Lysozyme Antibody (ARQ4974) [NBP3-15338] - Western blot analysis of extracts of various cell lines, using Lysozyme antibody (NBP3-15338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Lysozyme Antibody (ARQ4974) [NBP3-15338] - Immunohistochemistry of paraffin-embedded mouse intestin using Lysozyme (Lysozyme) Rabbit mAb (NBP3-15338) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(ARQ4974)-Immunohistochemistry-Paraffin-NBP3-15338-img0001.jpg)
![Western Blot: Lysozyme Antibody (LYZ/3944) [NBP3-13962] - Western blot analysis of human spleen tissue and HepG2 cell lysates using Lysozyme antibody (LYZ/3944).](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LYZ-3944)-Western-Blot-NBP3-13962-img0001.jpg "Western Blot: Lysozyme Antibody (LYZ/3944) [NBP3-13962] - Western blot analysis of human spleen tissue and HepG2 cell lysates using Lysozyme antibody (LYZ/3944).")
![Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3944) [NBP3-13962] - Formalin-fixed, paraffin-embedded human spleen stained with Lysozyme antibody (LYZ/3944). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(LYZ-3944)-Immunohistochemistry-Paraffin-NBP3-13962-img0002.jpg)
![Western Blot: Lysozyme Antibody (9B2O6) [NBP3-15290] - Western blot analysis of extracts of various cell lines, using Lysozyme (LYZ) Rabbit mAb (NBP3-15290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(9B2O6)-Western-Blot-NBP3-15290-img0002.jpg "Western Blot: Lysozyme Antibody (9B2O6) [NBP3-15290] - Western blot analysis of extracts of various cell lines, using Lysozyme (LYZ) Rabbit mAb (NBP3-15290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Lysozyme Antibody (9B2O6) [NBP3-15290] - Immunofluorescence analysis of human lung cancer using Lysozyme (LYZ) Rabbit mAb (NBP3-15290) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(9B2O6)-Immunocytochemistry-Immunofluorescence-NBP3-15290-img0001.jpg)
![Western Blot: Lysozyme Antibody (1C9) [NBP2-45702] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lysozyme.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(1C9)-Western-Blot-NBP2-45702-img0002.jpg "Western Blot: Lysozyme Antibody (1C9) [NBP2-45702] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lysozyme.")
![Immunohistochemistry: Lysozyme Antibody (1C9) [NBP2-45702] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-(1C9)-Immunohistochemistry-NBP2-45702-img0001.jpg)
![Western Blot: Lysozyme Overexpression Lysate (Adult Normal) [NBL1-12771] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lysozyme.](http://images.novusbio.com/fullsize/Lysozyme-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-12771-img0002.jpg "Western Blot: Lysozyme Overexpression Lysate (Adult Normal) [NBL1-12771] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lysozyme.")
![ELISA: Human Lysozyme ELISA Kit (Colorimetric) [NBP2-60513] - Standard curve.](http://images.novusbio.com/fullsize2/Human-Lysozyme-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60513-img0001.jpg "ELISA: Human Lysozyme ELISA Kit (Colorimetric) [NBP2-60513] - Standard curve.")