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![Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Huntingtin protein stained by Huntingtin antibody (NBP3-13106) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13106-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-13106-img0002.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0001.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0004.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0005.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(7B9C8)-Western-Blot-NBP3-15642-img0005.jpg "Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunofluorescence analysis of mouse brain using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(7B9C8)-Immunohistochemistry-NBP3-15642-img0004.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(7B9C8)-Western-Blot-NBP3-15642-img0006.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunohistochemistry of paraffin-embedded rat brain using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(7B9C8)-Immunohistochemistry-Paraffin-NBP3-15642-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(7B9C8)-Immunohistochemistry-Paraffin-NBP3-15642-img0002.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunofluorescence analysis of rat brain using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(7B9C8)-Immunohistochemistry-NBP3-15642-img0003.jpg)
![Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(JB89-34)-Western-Blot-NBP2-75531-img0006.jpg "Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(JB89-34)-Immunohistochemistry-Paraffin-NBP2-75531-img0005.jpg)
![Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(JB89-34)-Flow-Cytometry-NBP2-75531-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(JB89-34)-Immunohistochemistry-Paraffin-NBP2-75531-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(JB89-34)-Immunohistochemistry-Paraffin-NBP2-75531-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-(JB89-34)-Immunohistochemistry-Paraffin-NBP2-75531-img0004.jpg)
![Western Blot: Huntingtin Antibody [NBP1-44266] - Western blot analysis on mouse brain extract using 0.1ug/mL of NBP1-44266 (lane 1) and 0.1ug/mL of NBP1-44266 combined with 1ug/mL of blocking peptide (lane 2).](http://images.novusbio.com/fullsize/135219_1.jpg "Western Blot: Huntingtin Antibody [NBP1-44266] - Western blot analysis on mouse brain extract using 0.1ug/mL of NBP1-44266 (lane 1) and 0.1ug/mL of NBP1-44266 combined with 1ug/mL of blocking peptide (lane 2).")
![Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-15492-img0003.jpg "Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Huntingtin Antibody [NBP3-15492] - Immunofluorescence analysis of U-251 MG cells using Huntingtin Rabbit pAb (NBP3-15492) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15492-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-15492] - Immunohistochemistry of paraffin-embedded rat brain using Huntingtin Rabbit pAb (NBP3-15492) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-15492-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP1-89710] - Staining in human cerebral cortex and liver tissues using anti-HTT antibody. Corresponding HTT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP1-89710-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP1-89710] - Staining in human cerebral cortex and liver tissues using anti-HTT antibody. Corresponding HTT RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Huntingtin Antibody [NBP1-89710] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89710-img0005.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP1-89710] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP1-89710-img0006.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP1-89710] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP1-89710-img0007.jpg)
![Western Blot: Huntingtin Antibody (HDB4E10) [NB600-1197] - Western Blot analysis of normal human cerebral cortex total protein extract run on a 3-12.5% gradient SDS-PAGE gel and probed with Mouse anti Huntingtin](http://images.novusbio.com/fullsize/Huntingtin-Antibody-(HDB4E10)-Western-Blot-NB600-1197-img0001.jpg "Western Blot: Huntingtin Antibody (HDB4E10) [NB600-1197] - Western Blot analysis of normal human cerebral cortex total protein extract run on a 3-12.5% gradient SDS-PAGE gel and probed with Mouse anti Huntingtin")
![SDS-Page: Recombinant Human Huntingtin Protein [H00003064-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-Huntingtin-Protein-H00003064-Q01-img0001.jpg "SDS-Page: Recombinant Human Huntingtin Protein [H00003064-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")