| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48484 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 2279-BT-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF690-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 690-PT-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6903-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB410-SP | |||
| Carrier Free | MAB410-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27351SS | |||
| Azide and BSA Free | NBP2-80984 | |||
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-52B83-Immunohistochemistry-Paraffin-NB600-1422-img0001.jpg "Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.")
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![N/A TNF-alpha [Biotin]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_DY1814_ELISA_1720.jpg "N/A TNF-alpha [Biotin]")
![Western Blot: TNF-alpha Antibody [NBP2-44062] - Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Goat anti-Rabbit added at 1:20,000 in blocking buffer for 30 min RT.](http://images.novusbio.com/fullsize/TNF-alpha Antibody-Western Blot-NBP2-44062-img0011.jpg "Western Blot: TNF-alpha Antibody [NBP2-44062] - Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Goat anti-Rabbit added at 1:20,000 in blocking buffer for 30 min RT.")
![Immunohistochemistry: TNF-alpha Antibody [NBP2-44062] - Human prostate tissue was stained using TNFa antibody. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-NBP2-44062-img0010.jpg)
![Immunohistochemistry: TNF-alpha Antibody [NBP2-44062] - Fluorescent immunohistochemistry showing staining of human colon.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-NBP2-44062-img0006.jpg)
![Immunohistochemistry: TNF-alpha Antibody [NBP2-44062] - Staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-NBP2-44062-img0009.jpg)
![Western Blot: TNF-alpha Antibody (6N2E12) [NBP2-27351] - Analysis of TNF alpha recombinant protein using TNF alpha antibody at 3 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N2E12-Western-Blot-NBP2-27351-img0003.jpg "Western Blot: TNF-alpha Antibody (6N2E12) [NBP2-27351] - Analysis of TNF alpha recombinant protein using TNF alpha antibody at 3 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N2E12) [NBP2-27351] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27351. Human PBMC were stimulated with PMA and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with before testing with AF647 conjugate.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N2E12-Flow-Cytometry-NBP2-27351-img0005.jpg)