Tnf Products By Gene Name

1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF-a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF-a Monoclonal Antibody (Catalog # MAB1507). The ND₅₀ is typically 1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF-a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF-a Monoclonal Antibody (Catalog # MAB1507). The ND₅₀ is typically 1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Canine TNF-a (Catalog # 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF-a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF-a Monoclonal Antibody (Catalog # MAB1507). The ND₅₀ is typically 1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

210-TA) promotes cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 25-100 pg/mL. The similarity in activity highlights that the biotinylated protein is fully functional." class="big_lightbox"> 210-TA) promotes cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 25-100 pg/mL. The similarity in activity highlights that the biotinylated protein is fully functional." alt="Both Biotinylated Recombinant Human TNF-a (Catalog # BT210) and unlabeled Recombinant Human TNF-a (Catalog # 210-TA) promotes cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 25-100 pg/mL. The similarity in activity highlights that the biotinylated protein is fully functional." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TNF-a was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Porcine TNF‑ alpha (Catalog # 690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-a (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND₅₀ is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-a (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND₅₀ is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Canine TNF-a (1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-a (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND₅₀ is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />