Hu,
Mu,
Rt,
Po,
MaSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Ma
WB, Simple Western, Flow, ICC/IF, IP, Mycoplasma
Applications: WB, Simple Western, Flow, ICC/IF, IP, Mycoplasma
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - Analysis of TPX2 at the mitotic spindle microtubules and poles in a HeLa metaphase cell. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunocytochemistry-Immunofluorescence-NB500-179-img0004.jpg "Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - Analysis of TPX2 at the mitotic spindle microtubules and poles in a HeLa metaphase cell. ICC/IF image submitted by a verified customer review.")
![Flow Cytometry: TPX2 Antibody [NB500-179] - RHAMM is not a cell surface but an intracellular cytoskeletal protein in mouse embryonic stem (ES) cells. (C) Alcohol permeabilized mouse ES cells, however, were strongly positive for both RHAMM and the intracellular positive control protein TPX2 (right panel). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0073548) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Flow-Cytometry-NB500-179-img0005.jpg)
![Western Blot: TPX2 Antibody [NB500-179] - Analysis of TPX2 expression in 1) HeLa, 2) Ntera2, 3) K-562 and 4) Raji whole cell lysates using NB500-179.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - Staining of HeLa cells fixed in 3.5% paraformaldehyde using NB 500-179 (1:1,000). Nuclear staining during interphase and spindle staining during mitosis.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunocytochemistry-Immunofluorescence-NB500-179-img0003.jpg)
![Simple Western: TPX2 Antibody [NB500-179] - Lane view shows a specific band for TPX2 using HeLa cell lysate and antibody at 1:250. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.](http://images.novusbio.com/fullsize/TPX2-Antibody-Simple-Western-NB500-179-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - Depletion of TPX2 or Aurora-A reduces cell viability of BRCA2-deficient breast cancer cells. a HCC1806-shBRCA2dox, HCC38-shBRCA2dox, SUM149-shBRCA2dox, & MB231-shBRCA2dox were grown on coverslips & treated with doxycycline (3 days) and/or irradiated (IR, 5 Gy) as indicated. Subsequently, cells were stained for RAD51 & gamma H2AX. Scale bars represent 5 μm. b Quantification of results from a. Percentages of cells with ≥5 RAD51 foci per nucleus are indicated (n ≥ 31). c Percentages of cell survival of doxycycline-treated cells vs untreated cells, transfected with indicated siRNAs. Unpaired two-tailed t tests were used to test for statistical significance (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). d BT-549 cells were transfected with siTPX2 or control siRNA (CTR). Cells were grown on coverslips for 3 days after which they were incubated with EdU conjugated to azide-Alexa 488 (10 μM) for 15 min. Subsequently, cells were fixed & stained for 53BP1 & gamma H2AX. Amounts of 53BP1 & gamma H2AX foci per cell of at least 30 EdU-positive cells were counted. Means & standard deviations are depicted. Mann–Whitney U tests were used to analyze statistical significance (*p ≤ 0.05, ** = p ≤ 0.01, ***p ≤ 0.001, ns not significant). e BT-549 cells were transfected as in d, irradiated (IR, 5 Gy), & fixated 0.5 or 6 h after irradiation. Amounts of 53BP1 & gamma H2AX foci per cell were counted. Means & standard deviations are depicted. Mann–Whitney U tests were used to analyze statistical significance (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ns = not significant) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30177840), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-179_rabbit-polyclonal-tpx2-antibody-31020241616051.jpg)
![Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - TPX2 depletion preferentially affects cell viability in BRCA2-deficient cancer cells. a BT-549-shBRCA2dox cells were left untreated or were treated with doxycycline (2 or 4 days), & subsequently harvested for western blotting for BRCA2 & actin. b BT-549-shBRCA2dox cells were treated as in panel A, & mRNA expression levels of BRCA2 were analyzed relative to GAPDH using qRT-PCR. c BT-549-shBRCA2dox cells were grown on coverslips & treated with doxycycline (3 days) and/or irradiated (IR, 5 Gy) as indicated. At 3 h after irradiation, cells were fixed & analyzed for RAD51 & gamma H2AX foci formation. Scale bars represent 5 μm. d Percentages of cells with ≥5 RAD51 foci per nucleus are indicated. (n ≥ 50 per condition). e BT-549-shBRCA2dox cells were treated with doxycycline (3 days) & were subsequently transfected with indicated siRNAs. A total of 30,000 cells were plated 48 h following transfection. Viable cells were counted 5 days later. Percentages of cell survival of doxycycline-treated vs untreated cells are depicted. Error bars indicate standard deviations of two experimental replicates. Unpaired two-tailed t tests were used to test for statistical significance (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30177840), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-179_rabbit-polyclonal-tpx2-antibody-310202416171435.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0005.jpg "Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TPX2 Antibody [NB500-184] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-184. TPX2 was also immunoprecipitated using NB500-183 at 0.4 ug/mg lysate.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels. TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels Depletion of TPX2 by siRNA causes a decrease in H4K16ac levels whereas the levels of H3K9ac and H3K56ac remain unchanged. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Knockdown-Validated-NB500-184-img0004.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416182636.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416161844.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416171433.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416173534.jpg)
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NBP1-88312] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88312-img0014.jpg "Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NBP1-88312] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0017.jpg)
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0020.jpg)
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0018.jpg)
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human skin shows strong nuclear positivity in basal cells.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0019.jpg)
![Western Blot: TPX2 Antibody (TPX2-01) [NBP2-67265] - Analysis of TPX2 using monoclonal antibody TPX2 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Western-Blot-NBP2-67265-img0001.jpg "Western Blot: TPX2 Antibody (TPX2-01) [NBP2-67265] - Analysis of TPX2 using monoclonal antibody TPX2 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.")
![Immunocytochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - Glioblastoma cell line T98G: a) interphase, b) mitosis. Bar: 20 um.](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Immunocytochemistry-NBP2-67265-img0003.jpg)
![Immunohistochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Immunohistochemistry-NBP2-67265-img0004.jpg)
![Immunoprecipitation: TPX2 Antibody (TPX2-01) [NBP2-67265] - TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Immunoprecipitation-NBP2-67265-img0002.jpg)
![Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.](http://images.novusbio.com/fullsize/TPX2-Antibody-18D5-1-Western-Blot-NB100-74556-img0002.jpg "Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.")
![Immunohistochemistry-Paraffin: TPX2 Antibody (18D5-1) [NB100-74556] - Biopsies of deparaffinized Human testis tissue.](http://images.novusbio.com/fullsize/TPX2-Antibody-18D5-1-Immunohistochemistry-Paraffin-NB100-74556-img0003.jpg)
![Western Blot: TPX2 Antibody [NB500-183] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-183. TPX2 was also immunoprecipitated using NB500-184 at 0.4 ug/mg lysate.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-183-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TPX2 Antibody [NB500-183] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-183. TPX2 was also immunoprecipitated using NB500-184 at 0.4 ug/mg lysate.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-183] - HeLa cell. Antibody: Affinity purified rabbit anti- TPX2 used at a dilution of 1:400 (2.5ug/ml). Detection: Red-fluorescent goat anti-rabbit IgGheavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunocytochemistry-Immunofluorescence-NB500-183-img0004.jpg)
![Western Blot: TPX2 Antibody (S01-8A6) [NBP3-19922] - Western blot detection of TPX2 in Hela, A549, HL-60 using NBP3-19922 (1:1000 diluted)](http://images.novusbio.com/fullsize/TPX2-Antibody-S01-8A6-Western-Blot-NBP3-19922-img0003.jpg "Western Blot: TPX2 Antibody (S01-8A6) [NBP3-19922] - Western blot detection of TPX2 in Hela, A549, HL-60 using NBP3-19922 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: TPX2 Antibody (S01-8A6) [NBP3-19922] - Immunohistochemistry of TPX2 in paraffin-embedded Human lung cancer tissue using NBP3-19922 at dilution 1/50](http://images.novusbio.com/fullsize/TPX2-Antibody-S01-8A6-Immunohistochemistry-Paraffin-NBP3-19922-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody (S01-8A6) [NBP3-19922] - Immunocytochemistry of TPX2 (green) in Hela using NBP3-19922 at dilution 1/2, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19922_rabbit-tpx2-mab-s01-8a6-113202515145451.jpg)
![Western Blot: TPX2 Antibody (S05-1B5) [NBP3-19921] - Western blot detection of TPX2 in Hela, A549, HL-60 cell lysates using NBP3-19921 (1:1000 diluted). Predicted band size: 86kDa. Observed band size: 100kDa.](http://images.novusbio.com/fullsize/TPX2-Antibody-S05-1B5-Western-Blot-NBP3-19921-img0001.jpg "Western Blot: TPX2 Antibody (S05-1B5) [NBP3-19921] - Western blot detection of TPX2 in Hela, A549, HL-60 cell lysates using NBP3-19921 (1:1000 diluted). Predicted band size: 86kDa. Observed band size: 100kDa.")
![SDS-Page: Recombinant Human TPX2 Protein [H00022974-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00022974-Q01-1.jpg "SDS-Page: Recombinant Human TPX2 Protein [H00022974-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TPX2 Overexpression Lysate (Adult Normal) [NBL1-17236] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TPX2.](http://images.novusbio.com/fullsize/TPX2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17236-img0002.jpg "Western Blot: TPX2 Overexpression Lysate (Adult Normal) [NBL1-17236] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TPX2.")