| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-21514-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90388-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81115-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11987-0.025mg | |||
![Western Blot: 4-1BB Ligand/TNFSF9 Antibody (BLR148J) [NBP3-14727] - Whole cell lysate (25 ug) from HEK293T, Jurkat, Raji, and HeLa cells prepared using NETN lysis buffer. Rabbit anti-4-1BBL/CD137L/TNFSF9 recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-BLR148J-Western-Blot-NBP3-14727-img0002.jpg "Western Blot: 4-1BB Ligand/TNFSF9 Antibody (BLR148J) [NBP3-14727] - Whole cell lysate (25 ug) from HEK293T, Jurkat, Raji, and HeLa cells prepared using NETN lysis buffer. Rabbit anti-4-1BBL/CD137L/TNFSF9 recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.")
![Flow Cytometry: 4-1BB Ligand/TNFSF9 Antibody (BLR148J) [NBP3-14727] - Rabbit anti-4-1BBL/CD137L/TNFSF9 recombinant monoclonal or isotype control (unshaded). DyLight® 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-BLR148J-Flow-Cytometry-NBP3-14727-img0001.jpg)
![Western Blot: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-Western-Blot-NBP2-20673-img0002.jpg "Western Blot: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.")
![Immunoprecipitation: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673] - IP samples: A549 membrane extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of TNFSF9 protein by 4 ug TNFSF9 antibody [N2C3] 15 % SDS-PAGE The immunoprecipitated TNFSF9 protein was detected by TNFSF9 antibody [N2C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-Immunoprecipitation-NBP2-20673-img0004.jpg)
![Western Blot: 4-1BB Ligand/TNFSF9 Antibody (RM0067-3H19) - Azide and BSA Free [NBP1-21514] - Lysate of mouse embryonic tissue with rat anti-mouse 4-1BB Ligand/TNFSF9 antibody.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-RM0067-3H19-Azide-and-BSA-Free-NBP1-21514-img0001.jpg "Western Blot: 4-1BB Ligand/TNFSF9 Antibody (RM0067-3H19) - Azide and BSA Free [NBP1-21514] - Lysate of mouse embryonic tissue with rat anti-mouse 4-1BB Ligand/TNFSF9 antibody.")
![Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-014-Immunocytochemistry-Immunofluorescence-NBP2-90388-img0001.jpg "Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Analysis of Mouse TNFSF9 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse TNFSF9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-014-Flow-Cytometry-NBP2-90388-img0002.jpg)
![Western Blot: 4-1BB Ligand/TNFSF9 Antibody (JE33-91) [NBP3-31986] - Western blot analysis of 4-1BB Ligand/TNFSF9 on different lysates with Rabbit anti-4-1BB Ligand/TNFSF9 antibody (NBP3-31986) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 100nM TPA overnight whole cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 29 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31986) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31986_rabbit-4-1bb-ligand-tnfsf9-mab-je33-91-3920248485027.jpg "Western Blot: 4-1BB Ligand/TNFSF9 Antibody (JE33-91) [NBP3-31986] - Western blot analysis of 4-1BB Ligand/TNFSF9 on different lysates with Rabbit anti-4-1BB Ligand/TNFSF9 antibody (NBP3-31986) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 100nM TPA overnight whole cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 29 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31986) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody [NBP2-56103] - Staining of human cell line SiHa shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56103-img0001.jpg "Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody [NBP2-56103] - Staining of human cell line SiHa shows localization to the Golgi apparatus.")
![Western Blot: 4-1BB Ligand/TNFSF9 Overexpression Lysate [NBL1-17171] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF9.](http://images.novusbio.com/fullsize/TNFSF9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17171-img0002.jpg "Western Blot: 4-1BB Ligand/TNFSF9 Overexpression Lysate [NBL1-17171] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF9.")
![ELISA: 4-1BB Ligand/TNFSF9 Antibody (TKS-1) [NBP2-81115] - ELISA of anti-4-1BBL antibody on 4-1BBL-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-4-1BBL antibody TKS-1 (NBP2-81115; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse 4-1BBL Fc-fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using NBP2-81115. For signal detection, a 1:4000 dilution of HRP-fused anti-rabbit IgG antibody was used.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-TKS-1-ELISA-NBP2-81115-img0001.jpg "ELISA: 4-1BB Ligand/TNFSF9 Antibody (TKS-1) [NBP2-81115] - ELISA of anti-4-1BBL antibody on 4-1BBL-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-4-1BBL antibody TKS-1 (NBP2-81115; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse 4-1BBL Fc-fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using NBP2-81115. For signal detection, a 1:4000 dilution of HRP-fused anti-rabbit IgG antibody was used.")
![ELISA: Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric) [NBP3-38754] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38754_human-4-1bb-ligand-tnfsf9-elisa-kit-colorimetric-211020241537132.png "ELISA: Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric) [NBP3-38754] - Standard Curve Reference")
![ELISA: Human 4-1BB Ligand/TNFSF9 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38753] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38753_human-4-1bb-ligand-tnfsf9-ready-to-use-elisa-kit-color-2110202415393798.png "ELISA: Human 4-1BB Ligand/TNFSF9 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38753] - Standard Curve Reference")