| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| CoraFluor 1 | NBP2-34539CL1 | |||
| FITC | NBP2-34539F | |||
| Janelia Fluor 525 | NBP2-34539JF525 | |||
| Janelia Fluor 669 | NBP2-34539JF669 | |||
| mFluor Violet 450 SE | NBP2-34539MFV450 | |||
| mFluor Violet 500 SE | NBP2-34539MFV500 | |||
| mFluor Violet 610 SE | NBP2-34539MFV610 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34539-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34419-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1507-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47675-0.1mg | |||
| IHC-Prediluted | NBP2-48470 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 410-TRNC-050/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 410-TRNC-010/CF | |||
| Carrier Free | 410-TRNC-050/CF | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB6902U-100UG | |||
| Alexa Fluor 405 | FAB6902V-100UG | |||
| Alexa Fluor 488 | FAB6902G-100UG | |||
| Alexa Fluor 532 | FAB6902X-100UG | |||
| Alexa Fluor 594 | FAB6902T-100UG | |||
| Alexa Fluor 647 | FAB6902R-100UG | |||
| Alexa Fluor 700 | FAB6902N-100UG | |||
| Alexa Fluor 750 | FAB6902S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6902-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2279-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1507-SP | |||
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (4C6-H8) [NBP2-34301] - Immunofluorescence staining of paraformaldehyde-fixed HePG2 cells with TNF-alpha Antibody (4C6-H8) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Red Dot (Red).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-4C6-H8-Immunocytochemistry-Immunofluorescence-NBP2-34301-img0001.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (4C6-H8) [NBP2-34301] - Immunofluorescence staining of paraformaldehyde-fixed HePG2 cells with TNF-alpha Antibody (4C6-H8) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Red Dot (Red).")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (4C6-H8) [NBP2-34301] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF-alpha Antibody (4C6-H8).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-4C6-H8-Immunohistochemistry-Paraffin-NBP2-34301-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (SPM543) [NBP2-34372] - Immunofluorescence staining of HepG2 cells using TNF-alpha Mouse Monoclonal Antibody (SPM543) followed by goat anti-mouse IgG-CF488 (green). Counterstain is RedDot.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-SPM543-Immunocytochemistry-Immunofluorescence-NBP2-34372-img0003.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (SPM543) [NBP2-34372] - Immunofluorescence staining of HepG2 cells using TNF-alpha Mouse Monoclonal Antibody (SPM543) followed by goat anti-mouse IgG-CF488 (green). Counterstain is RedDot.")
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Immunofluorescence Analysis of PFA-fixed HePG2 cells labeling TNF-alpha with TNF-alpha Antibody (TNFA/1172) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunocytochemistry-Immunofluorescence-NBP2-45331-img0007.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Immunofluorescence Analysis of PFA-fixed HePG2 cells labeling TNF-alpha with TNF-alpha Antibody (TNFA/1172) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Stomach stained with TNF alpha Monoclonal Antibody (TNFA/1172)](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunohistochemistry-Paraffin-NBP2-45331-img0005.jpg)
![Flow Cytometry: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using TNF-alpha Antibody (TNFA/1172).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Flow-Cytometry-NBP2-45331-img0006.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Human Erdheim Chester disease (also known as polyostotic lerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNFA/1172).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunohistochemistry-Paraffin-NBP2-45331-img0001.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNFA/1172)](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunohistochemistry-Paraffin-NBP2-45331-img0004.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Formalin-fixed, paraffin-embedded human Pancreas stained with TNF-alpha Antibody (TNFA/1172).](http://images.novusbio.com/fullsize/nbp2-45331_mouse-monoclonal-tnf-alpha-antibody-tnfa-1172-177202495945.jpg)
