Recombinant TEV Protease His (N-Term) Protein

• Applications: Enzyme Activity

Recombinant TEV Protease His (N-Term) Protein Summary

• Description: TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It is consists of 241 a.a. amino acids with the molecular weight of 27kDa. It contains N-terminal His-tag.
  
  

  Source: Escherichia coli.
  
  One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.
  
  TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X’, and cleaves between Q (or S)/X’. In this form X and X’ stand for any of the amino acid residues, except that X’ cannot be P. The optimal cleavage site is ENLYFQ/G. TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. In this form X and X' stand for any of the amino acid residues, except that X' cannot be P. The optimal cleavage site is ENLYFQ/G. As having the absolute specificity and wildly using conditions like broad pH range and ionic strength, the TEV protease became more versatile than EK, thrombin and other protease used in biochemical applications, especially recombinant protein production.

• Details of Functionality: One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85% completion at 30C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.The optimal temperature for cleavage is 30C; however, the enzyme can be used at temperatures as low as 4C. Reactions at 4C will require longer incubation times and/or more rTEV. If the protein of interest is heat-labile, then 4C incubations are recommended.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TEVgp1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Application Notes: The optimal temperature for cleavage is 30C; however, the enzyme can be used at temperatures as low as 4C.
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 5 mM EDTA, 10 mM GSH
• Preservative: 50% glycerol
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant TEV Protease His (N-Term) Protein

• NIa
• P1 Protease
• TEV Protease
• Tobacco Etch Virus Protease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TEVgp1