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NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="E-Selectin/CD62E was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) activated with TNF alpha using Mouse Anti-Human E-Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # BBA8) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

ADP1) was serially diluted and captured by Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> ADP1) was serially diluted and captured by Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse E‑Selectin/CD62E Fc Chimera (Catalog # 575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />