| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20774 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37423-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73165 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89952-25ul | |||
1433-OP) was serially diluted and captured by Mouse Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB14332) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1433) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Osteopontin/OPN (Catalog # 1433-OP) was serially diluted and captured by Mouse Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB14332) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1433) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB14332-SP | |||
| Carrier Free | MAB14332-500 | |||
![Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Western-Blot-NBP2-37423-img0003.jpg "Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis of endometrial cancer tissues using SPP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Immunohistochemistry-NBP2-37423-img0005.jpg)
![Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Western-Blot-NBP2-37423-img0002.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis of prostate cancer tissues using SPP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Immunohistochemistry-NBP2-37423-img0004.jpg)
![ELISA: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-ELISA-NBP2-37423-img0001.jpg)
![Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NB100-1883-img0001.jpg "Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Osteopontin.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Western-Blot-NBP2-45954-img0009.jpg "Western Blot: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Osteopontin.")
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human prostate tissue using SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0008.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0001.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0002.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0003.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0004.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0005.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0006.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0007.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0003.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443] - Analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 ug,) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Flow-Cytometry-NBP2-89443-img0001.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0007.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0016.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human tonsil shows low positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0017.jpg)
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0002.jpg "Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-59190-img0003.jpg)
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0004.jpg)
![ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] - Samples were spiked with high concentrations of Monkey Osteopontin/OPN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68076_monkey-osteopontin-opn-elisa-kit-colorimetric-132202416102286.jpg "ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] - Samples were spiked with high concentrations of Monkey Osteopontin/OPN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68076_monkey-osteopontin-opn-elisa-kit-colorimetric-4320249151664.jpg)
