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![Western Blot: Surfactant Protein A Antibody [NBP2-12928] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Western-Blot-NBP2-12928-img0001.jpg "Western Blot: Surfactant Protein A Antibody [NBP2-12928] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - Sca-1+Abcg1+cells are the cancer stem cell-like subset of AT2 cells. Confocal analysis of SPA/ABCG1 and alpha6/beta4 in spheroid of Sca-1+Abcg1+cells isolated from KO mice, (Bar = 10 um). Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41388-020-1251-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-12928-img0005.jpg)
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928] - Staining of paraffin embedded Human Lung. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-Paraffin-NBP2-12928-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes the phosphorylation and acetylation of NF-kB and mediates the elevation of ABCG1 expression in AT2 cells. IF analysis of ABCG1 in the S/TB region of lung tissue from WT or KO mice, (Bar = 100 um). Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41388-020-1251-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-12928-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes NF-kB-mediated ECM1 expression elevation in Lgr5 cells and secreted ECM1-induced AT2 cell enrichment in the S/TB region. IF analysis of AT2 SPA (Surfactant Protein A) cells in the lung S/TB region after KO:Lgr5 cells with ECM1 or p65 knockdown and injected into NOD/SCID mice through tail vein, (Bar = 100 um); Data were collected from three independent experiments with triplicate samples. **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41388-020-1251-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-12928-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - ECM1-alpha 6 beta 4-ABCG1 axis is enriched in the S/TB region of the lungs in patients with pneumonia.IF analysis of GPRC5A, SPA/ABCG1, alpha 6/ beta 4, alpha 6/ECM1 & beta 4/ECM1 in normal or pneumonic lung tissue samples (a) & the calculation of positive or dual-positive cells in the S/TB region (b), (Bar = 100 µm); c Graphical abstract of the study: GPRC5A deficiency promotes the activation of NF-kB & subsequent expression & secretion of ECM1; the secreted ECM1 interacts with alpha 6 beta 4 of AT2 cells & induces the activation of NF-kB, which induces the expression of ABCG1. AT2 cells with ABCG1 expression are one of the originating cell populations of lung cancer. Data were collected from three independent experiments with triplicate samples. **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12928_goat-polyclonal-surfactant-protein-a-antibody-imgenex-img-31448-3102024161363.jpg)
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46679] - Analysis in human lung and liver tissues using NBP2-46679 antibody. Corresponding SFTPA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-Paraffin-NBP2-46679-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46679] - Analysis in human lung and liver tissues using NBP2-46679 antibody. Corresponding SFTPA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Surfactant Protein A Antibody [NBP2-46679] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Western-Blot-NBP2-46679-img0005.jpg)
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46679] - Staining of human Lung shows strong cytoplasmic positivity in pneumocytes.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-Paraffin-NBP2-46679-img0012.jpg)
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46679] - Staining of human rectum shows no positvity in glanular cells as expected.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-Paraffin-NBP2-46679-img0008.jpg)
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46679] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-Paraffin-NBP2-46679-img0009.jpg)
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46679] - Staining of human kidney shows no postivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-Paraffin-NBP2-46679-img0010.jpg)
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46679] - Analysis in human lung and liver tissues using NBP2-46679 antibody. Corresponding SFTPA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-Paraffin-NBP2-46679-img0011.jpg)
![Western Blot: Surfactant Protein A Antibody (RM334) [NBP2-77414] - Western Blot of human lung tissue lysate using NBP2-77414.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-RM334-Western-Blot-NBP2-77414-img0002.jpg "Western Blot: Surfactant Protein A Antibody (RM334) [NBP2-77414] - Western Blot of human lung tissue lysate using NBP2-77414.")
![Immunohistochemistry: Surfactant Protein A Antibody (RM334) [NBP2-77414] - Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using NBP2-77414.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-RM334-Immunohistochemistry-NBP2-77414-img0001.jpg)
![ELISA: Mouse Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76693] - Samples were spiked with high concentrations of Mouse Surfactant Protein A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76693_mouse-surfactant-protein-a-elisa-kit-colorimetric-13220241625170.jpg "ELISA: Mouse Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76693] - Samples were spiked with high concentrations of Mouse Surfactant Protein A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76693] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76693_mouse-surfactant-protein-a-elisa-kit-colorimetric-432024924098.jpg)
![SDS-Page: Recombinant Human Surfactant Protein A Protein [H00653509-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Surfactant-Protein-A-Protein-SDS-Page-H00653509-P01-img0002.jpg "SDS-Page: Recombinant Human Surfactant Protein A Protein [H00653509-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody (4C4) [H00653509-M06] - Analysis of monoclonal antibody to SFTPA1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00653509-M06-img0001.jpg "Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody (4C4) [H00653509-M06] - Analysis of monoclonal antibody to SFTPA1 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46720] - Staining of human lung shows strong cytoplasmic and membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-NBP2-46720-img0001.jpg "Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46720] - Staining of human lung shows strong cytoplasmic and membranous positivity in pneumocytes.")
![Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46771] - Staining of human lung shows strong cytoplasmic positivity in a fraction of pneumocytes.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Immunohistochemistry-NBP2-46771-img0001.jpg "Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-46771] - Staining of human lung shows strong cytoplasmic positivity in a fraction of pneumocytes.")
![Western Blot: Surfactant Protein A Antibody [NBP2-15130] - Sample (50 ug of whole cell lysate) A: mouse lung 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Antibody-Western-Blot-NBP2-15130-img0001.jpg "Western Blot: Surfactant Protein A Antibody [NBP2-15130] - Sample (50 ug of whole cell lysate) A: mouse lung 12% SDS PAGE, antibody diluted at 1:500.")
![ELISA: Rat Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76694] - Samples were spiked with high concentrations of Rat Surfactant Protein A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76694_rat-surfactant-protein-a-elisa-kit-colorimetric-132202416175672.jpg "ELISA: Rat Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76694] - Samples were spiked with high concentrations of Rat Surfactant Protein A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76694] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76694_rat-surfactant-protein-a-elisa-kit-colorimetric-4320249173448.jpg)
![ELISA: Human Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76692] - Samples were spiked with high concentrations of Human Surfactant Protein A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76692_human-surfactant-protein-a-elisa-kit-colorimetric-132202416201935.jpg "ELISA: Human Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76692] - Samples were spiked with high concentrations of Human Surfactant Protein A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Surfactant Protein A ELISA Kit (Colorimetric) [NBP2-76692] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76692_human-surfactant-protein-a-elisa-kit-colorimetric-43202495057.jpg)
![Western Blot: Surfactant Protein A Overexpression Lysate [NBP2-10468] - (Adult Normal) [NBP2-10468] Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for Surfactant Protein A.](http://images.novusbio.com/fullsize/Surfactant-Protein-A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10468-img0001.jpg "Western Blot: Surfactant Protein A Overexpression Lysate [NBP2-10468] - (Adult Normal) [NBP2-10468] Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for Surfactant Protein A.")