FAK: Novus Biologicals

NBP3-12897

FAK [p Tyr397] Antibody

100 ul

NBP3-12897

<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HUVEC whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: FAK [p Tyr397] Antibody [NBP3-12897] - FAK antibody detects FAK protein at cell junction, nucleus and cytoplasm by immunofluorescent analysis.</br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: FAK stained by FAK antibody (NBP3-12897) diluted at 1:500.</br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-67327

FAK Antibody (SR46-04)

(2 Publications)

100 ul

NBP2-67327

<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m

Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen

Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB4467

FAK Antibody (495919) [Unconjugated]

(2 Publications)

100 μg

MAB4467

HAF007). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

Western blot shows lysates of MCF-7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh-7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF007). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh-7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human FAK Monoclonal Antibody (Catalog # MAB4467) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Phosphorylation of FAK following treatment of AF cells derived from non-degenerate IVDs with 1.0 Hz CTS, with and without pre-treatment with RGD or RAD peptides.AF cells derived from non-degenerate IVDs (n = 4) were treated+/− RGD (50 µg/ml) or RAD (50 µg/ml) peptides, and mechanically stimulated (10% CTS, 1.0 Hz frequency) in serum-free media and total protein extracted at timepoints of 5 and 20 minutes. Mechanically stimulated and unstimulated,+/− RGD or RAD peptides, non-degenerate protein samples (5 µg/well) exposed to A) 5 minutes and B) 20 minutes of CTS, were separated using 10% SDS-PAGE and probed using primary antibodies against phosphorylated FAK. Blots were then stripped using a stripping buffer, re-blocked and probed using an antibody against total FAK protein. C) The density of bands were quantified using a Syngene imaging system and the ratio of phosphorylated: total FAK protein normalised to timepoint controls and plotted as % change. *denotes a significant change (p≤0.05) between treatment groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24039840), licensed under a CC-BY license. Not internally tested by R&D Systems.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FAK at approximately 120 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human FAK Monoclonal Antibody (Catalog # MAB4467) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4467U-100UG
Alexa Fluor 405	FAB4467V-100UG
Alexa Fluor 488	FAB4467G-100UG
Alexa Fluor 532	FAB4467X-100UG
Alexa Fluor 594	FAB4467T-100UG
Alexa Fluor 647	FAB4467R-100UG
Alexa Fluor 700	FAB4467N-100UG
Alexa Fluor 750	FAB4467S-100UG
Alexa Fluor Plus 405	FAB4467AFP405-100UG
Alexa Fluor Plus 488	FAB4467AFP488-100UG
Alexa Fluor Plus 555	FAB4467AFP555-100UG
Alexa Fluor Plus 594	FAB4467AFP594-100UG
Alexa Fluor Plus 647	FAB4467AFP647-100UG
Alexa Fluor Plus 680	FAB4467AFP680-100UG
Allophycocyanin	FAB4467A
Biotin	FAB4467B
CoraFluor 1	FAB4467CL1
DyLight 350	FAB4467D
DyLight 405	FAB4467E
DyLight 488	FAB4467K
DyLight 550	FAB4467L
DyLight 594	FAB4467M
DyLight 650	FAB4467W
DyLight 680	FAB4467Y
DyLight 755	FAB4467Z
FITC	FAB4467F
HRP	FAB4467H
Janelia Fluor 525	FAB4467JF525
Janelia Fluor 549	FAB4467I
Janelia Fluor 646	FAB4467J
Janelia Fluor 669	FAB4467JF669
PE	FAB4467P
PerCP	FAB4467C
mFluor Violet 450 SE	FAB4467MFV450
mFluor Violet 500 SE	FAB4467MFV500
mFluor Violet 610 SE	FAB4467MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4467-SP

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AF4467

FAK Antibody [Unconjugated]

(3 Publications)

100 μg

AF4467

HAF016). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of MCF‑7 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of MCF‑7 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 3 µg/mL Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF016). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF016). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

ADAM9 affects auto‐/paracrine MEK/ERK signaling. (A) Western blot of phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in AsPC‐1 and MiaPaCa‐2 cells following ADAM9 expression silencing. In AsPC‐1 cells, we observed decreased pFAK (Y397), which was absent in MiaPaCa‐2 cells. Quantitation of pFAK, pMEK1/2, and pERK1/2 in AsPc‐1 and MiaPaCa‐2 cells from three independent experiments. (B) Western blot analysis shows decreased phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in HUVEC cells following 30 min of incubation with cancer cell conditioned medium from both AsPC‐1 and MiaPaCa‐2 cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30556643), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4467U-100UG
Alexa Fluor 405	AF4467V-100UG
Alexa Fluor 488	AF4467G-100UG
Alexa Fluor 532	AF4467X-100UG
Alexa Fluor 594	AF4467T-100UG
Alexa Fluor 647	AF4467R-100UG
Alexa Fluor 700	AF4467N-100UG
Alexa Fluor 750	AF4467S-100UG
Alexa Fluor Plus 405	AF4467AFP405-100UG
Alexa Fluor Plus 488	AF4467AFP488-100UG
Alexa Fluor Plus 555	AF4467AFP555-100UG
Alexa Fluor Plus 594	AF4467AFP594-100UG
Alexa Fluor Plus 647	AF4467AFP647-100UG
Alexa Fluor Plus 680	AF4467AFP680-100UG
Allophycocyanin	AF4467A
Biotin	AF4467B
CoraFluor 1	AF4467CL1
DyLight 350	AF4467D
DyLight 405	AF4467E
DyLight 488	AF4467K
DyLight 550	AF4467L
DyLight 594	AF4467M
DyLight 650	AF4467W
DyLight 680	AF4467Y
DyLight 755	AF4467Z
FITC	AF4467F
HRP	AF4467H
Janelia Fluor 525	AF4467JF525
Janelia Fluor 549	AF4467I
Janelia Fluor 646	AF4467J
Janelia Fluor 669	AF4467JF669
PE	AF4467P
PerCP	AF4467C
mFluor Violet 450 SE	AF4467MFV450
mFluor Violet 500 SE	AF4467MFV500
mFluor Violet 610 SE	AF4467MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4467-SP

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NBP2-45923

FAK Antibody (OTI4D11)

0.1 ml

NBP2-45923

Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunocytochemistry/Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923] - FAK Antibody (4D11) [NBP2-45923] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY FAK.

Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71268AF350
Alexa Fluor 405	NBP2-71268AF405
Alexa Fluor 488	NBP2-71268AF488
Alexa Fluor 532	NBP2-71268AF532
Alexa Fluor 594	NBP2-71268AF594
Alexa Fluor 647	NBP2-71268AF647
Alexa Fluor 700	NBP2-71268AF700
Alexa Fluor 750	NBP2-71268AF750
Alexa Fluor Plus 405	NBP2-71268AFP405
Alexa Fluor Plus 488	NBP2-71268AFP488
Alexa Fluor Plus 555	NBP2-71268AFP555
Alexa Fluor Plus 594	NBP2-71268AFP594
Alexa Fluor Plus 647	NBP2-71268AFP647
Alexa Fluor Plus 680	NBP2-71268AFP680
Allophycocyanin	NBP2-71268APC
Biotin	NBP2-71268B
CoraFluor 1	NBP2-71268CL1
DyLight 350	NBP2-71268UV
DyLight 405	NBP2-71268V
DyLight 488	NBP2-71268G
DyLight 550	NBP2-71268R
DyLight 594	NBP2-71268DL594
DyLight 650	NBP2-71268C
DyLight 680	NBP2-71268FR
DyLight 755	NBP2-71268IR
FITC	NBP2-71268F
HRP	NBP2-71268H
Janelia Fluor 525	NBP2-71268JF525
Janelia Fluor 549	NBP2-71268JF549
Janelia Fluor 646	NBP2-71268JF646
Janelia Fluor 669	NBP2-71268JF669
PE	NBP2-71268PE
PerCP	NBP2-71268PCP
mFluor Violet 450 SE	NBP2-71268MFV450
mFluor Violet 500 SE	NBP2-71268MFV500
mFluor Violet 610 SE	NBP2-71268MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71268

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MAB4528

FAK [p Tyr397] Antibody (820755)

(4 Publications)

100 μg

MAB4528

HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>ADAM9 affects auto‐/paracrine MEK/ERK signaling. (A) Western blot of phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in AsPC‐1 and MiaPaCa‐2 cells following ADAM9 expression silencing. In AsPC‐1 cells, we observed decreased pFAK (Y397), which was absent in MiaPaCa‐2 cells. Quantitation of pFAK, pMEK1/2, and pERK1/2 in AsPc‐1 and MiaPaCa‐2 cells from three independent experiments. (B) Western blot analysis shows decreased phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in HUVEC cells following 30 min of incubation with cancer cell conditioned medium from both AsPC‐1 and MiaPaCa‐2 cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30556643), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4528U-100UG
Alexa Fluor 405	FAB4528V-100UG
Alexa Fluor 488	FAB4528G-100UG
Alexa Fluor 532	FAB4528X-100UG
Alexa Fluor 594	FAB4528T-100UG
Alexa Fluor 647	FAB4528R-100UG
Alexa Fluor 700	FAB4528N-100UG
Alexa Fluor 750	FAB4528S-100UG
Alexa Fluor Plus 405	FAB4528AFP405-100UG
Alexa Fluor Plus 488	FAB4528AFP488-100UG
Alexa Fluor Plus 555	FAB4528AFP555-100UG
Alexa Fluor Plus 594	FAB4528AFP594-100UG
Alexa Fluor Plus 647	FAB4528AFP647-100UG
Alexa Fluor Plus 680	FAB4528AFP680-100UG
Allophycocyanin	FAB4528A
Biotin	FAB4528B
CoraFluor 1	FAB4528CL1
DyLight 350	FAB4528D
DyLight 405	FAB4528E
DyLight 488	FAB4528K
DyLight 550	FAB4528L
DyLight 594	FAB4528M
DyLight 650	FAB4528W
DyLight 680	FAB4528Y
DyLight 755	FAB4528Z
FITC	FAB4528F
HRP	FAB4528H
Janelia Fluor 525	FAB4528JF525
Janelia Fluor 549	FAB4528I
Janelia Fluor 646	FAB4528J
Janelia Fluor 669	FAB4528JF669
PE	FAB4528P
PerCP	FAB4528C
mFluor Violet 450 SE	FAB4528MFV450
mFluor Violet 500 SE	FAB4528MFV500
mFluor Violet 610 SE	FAB4528MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4528-SP

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NBP3-13071

FAK [p Tyr576] Antibody

100 ul

NBP3-13071

Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: FAK [p Tyr576] Antibody [NBP3-13071] - FAK antibody detects FAK protein at cell membrane by immunohistochemical analysis.</br>Sample: Paraffin-embedded rat lung.</br>FAK stained by FAK antibody (NBP3-13071) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis.<br>Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>Scale bar = 10 um.

Reactivity:

Hu, Mu, Rt, Ch, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Xp

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-84750

FAK Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-84750

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human testis shows moderate to cytoplasmic positivity in spermatogonia and Leydig cells.

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression & metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta 1 (a) or beta 4 (b) receptor (green) & FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 μm. (c) MS imaging of cross sections of the medium-conditioned blank (upper panels) & MM231 cell culture-laden (bottom panels) hydrogel scaffolds. (d) Examples of ROC (upper) & box-and-whiskers (bottom) plots from the MS imaging analysis, showing data for a compound with m/z 663.024, where differences between the hydrogel samples cultured with or without MM231 cells are clearly evident. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30257480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-84750-25ul

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NBP3-13085

FAK [p Tyr407] Antibody

100 ul

NBP3-13085

Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Untreated (-) and treated (+) NHI-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000 and Fak antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br>

Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000 and FAK antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br>

Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000 and FAK antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br>

Reactivity:

Hu, Mu, Rt, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-13675

FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

100 ul

NBP3-13675

Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody [HL127] (NBP3-13675) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB7298

FAK [p Ser843] Antibody (743101)

(1 Publication)

100 μg

MAB7298

HAF007). A specific band was detected for Phospho-FAK (S843) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 1 mM Pervanadate (PV) 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-FAK (S843) Monoclonal Antibody (Catalog # MAB7298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Phospho-FAK (S843) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 1 mM Pervanadate (PV) 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-FAK (S843) Monoclonal Antibody (Catalog # MAB7298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-FAK (S843) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7298U-100UG
Alexa Fluor 405	FAB7298V-100UG
Alexa Fluor 488	FAB7298G-100UG
Alexa Fluor 532	FAB7298X-100UG
Alexa Fluor 594	FAB7298T-100UG
Alexa Fluor 647	FAB7298R-100UG
Alexa Fluor 700	FAB7298N-100UG
Alexa Fluor 750	FAB7298S-100UG
Alexa Fluor Plus 405	FAB7298AFP405-100UG
Alexa Fluor Plus 488	FAB7298AFP488-100UG
Alexa Fluor Plus 555	FAB7298AFP555-100UG
Alexa Fluor Plus 594	FAB7298AFP594-100UG
Alexa Fluor Plus 647	FAB7298AFP647-100UG
Alexa Fluor Plus 680	FAB7298AFP680-100UG
Allophycocyanin	FAB7298A
Biotin	FAB7298B
CoraFluor 1	FAB7298CL1
DyLight 350	FAB7298D
DyLight 405	FAB7298E
DyLight 488	FAB7298K
DyLight 550	FAB7298L
DyLight 594	FAB7298M
DyLight 650	FAB7298W
DyLight 680	FAB7298Y
DyLight 755	FAB7298Z
FITC	FAB7298F
HRP	FAB7298H
Janelia Fluor 525	FAB7298JF525
Janelia Fluor 549	FAB7298I
Janelia Fluor 646	FAB7298J
Janelia Fluor 669	FAB7298JF669
PE	FAB7298P
PerCP	FAB7298C
mFluor Violet 450 SE	FAB7298MFV450
mFluor Violet 500 SE	FAB7298MFV500
mFluor Violet 610 SE	FAB7298MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7298-SP

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AF44671

FAK [p Tyr925] Antibody

(1 Publication)

100 μg

AF44671

HAF008). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FAK (Y925) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF44671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FAK (Y925) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF44671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF44671U-100UG
Alexa Fluor 405	AF44671V-100UG
Alexa Fluor 488	AF44671G-100UG
Alexa Fluor 532	AF44671X-100UG
Alexa Fluor 594	AF44671T-100UG
Alexa Fluor 647	AF44671R-100UG
Alexa Fluor 700	AF44671N-100UG
Alexa Fluor 750	AF44671S-100UG
Alexa Fluor Plus 405	AF44671AFP405-100UG
Alexa Fluor Plus 488	AF44671AFP488-100UG
Alexa Fluor Plus 555	AF44671AFP555-100UG
Alexa Fluor Plus 594	AF44671AFP594-100UG
Alexa Fluor Plus 647	AF44671AFP647-100UG
Alexa Fluor Plus 680	AF44671AFP680-100UG
Allophycocyanin	AF44671A
Biotin	AF44671B
CoraFluor 1	AF44671CL1
DyLight 350	AF44671D
DyLight 405	AF44671E
DyLight 488	AF44671K
DyLight 550	AF44671L
DyLight 594	AF44671M
DyLight 650	AF44671W
DyLight 680	AF44671Y
DyLight 755	AF44671Z
FITC	AF44671F
HRP	AF44671H
Janelia Fluor 525	AF44671JF525
Janelia Fluor 549	AF44671I
Janelia Fluor 646	AF44671J
Janelia Fluor 669	AF44671JF669
PE	AF44671P
PerCP	AF44671C
mFluor Violet 450 SE	AF44671MFV450
mFluor Violet 500 SE	AF44671MFV500
mFluor Violet 610 SE	AF44671MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF44671-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

PTK2 Products By Gene Name

FAK [p Tyr397] Antibody

FAK Antibody (SR46-04)

FAK Antibody (495919) [Unconjugated]

FAK Antibody [Unconjugated]

FAK Antibody (OTI4D11)

FAK [p Tyr397] Antibody (820755)

FAK [p Tyr576] Antibody

FAK Antibody - BSA Free

FAK [p Tyr407] Antibody

FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

FAK [p Ser843] Antibody (743101)

FAK [p Tyr925] Antibody