| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88660-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38155-25ul | |||
HAF019). A specific band was detected for PSMB10/MECL1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, HuT 78 human cutaneous T cell lymphoma cell line, and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PSMB10/MECL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PSMB10/MECL1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7684-SP | |||
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-88660] - Analysis in human tonsil and skeletal muscle tissues using NBP1-88660 antibody. Corresponding PSMB10 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP1-88660-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-88660] - Analysis in human tonsil and skeletal muscle tissues using NBP1-88660 antibody. Corresponding PSMB10 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PSMB10/MECL1 Antibody [NBP1-88660] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-450](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Western-Blot-NBP1-88660-img0005.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-88660] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP1-88660-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-88660] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP1-88660-img0009.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP2-38155] - Staining in human lymph node and cerebral cortex tissues using anti-PSMB10 antibody. Corresponding PSMB10 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38155-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP2-38155] - Staining in human lymph node and cerebral cortex tissues using anti-PSMB10 antibody. Corresponding PSMB10 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: PSMB10/MECL1 Antibody [NBP2-38155] - Staining of human tonsil shows nuclear positivity in non germinal center.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-NBP2-38155-img0002.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP2-38155] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38155-img0004.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP2-38155] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38155-img0005.jpg)
![Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Western-Blot-NBP1-51958-img0003.jpg "Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-NBP1-51958-img0005.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human tonsil. Antbody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-NBP1-51958-img0004.jpg)
![Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - PSMB10 monoclonal antibody (M01), clone 3F8 Analysis of PSMB10 expression in Jurkat.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-3F8-Western-Blot-H00005699-M01-img0007.jpg "Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - PSMB10 monoclonal antibody (M01), clone 3F8 Analysis of PSMB10 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Analysis of monoclonal antibody to PSMB10 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-3F8-Immunocytochemistry-Immunofluorescence-H00005699-M01-img0005.jpg)
![Sandwich ELISA: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Detection limit for recombinant GST tagged PSMB10 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-3F8-Sandwich-ELISA-H00005699-M01-img0006.jpg)
![Immunohistochemistry: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863] - Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10/MECL1 antibody (NBP3-32863) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32863_rabbit-psmb10-mecl1-mab-psh01-58-298202414161866.jpg "Immunohistochemistry: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863] - Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10/MECL1 antibody (NBP3-32863) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32863 at 1/1,000 dilution. Lane 1: HeLa treated with 100ng/mL IFN-gamma for 48 hours cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Daudi cell lysate (20 µg/Lane) Lane 4: Raji cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: Ramos cell lysate (20 µg/Lane) Lane 7: U-937 cell lysate (20 µg/Lane) Lane 8: HL-60 cell lysate (20 µg/Lane) Lane 9: Jurkat cell lysate (20 µg/Lane) Lane 10: SiHa cell lysate (20 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 25/29 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32863 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32863_-western-blot-639204595244768089.jpg)
![Western Blot: PSMB10 Antibody [NBP1-58937] - Human Placenta lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PSMB10-Antibody-Western-Blot-NBP1-58937-img0002.jpg "Western Blot: PSMB10 Antibody [NBP1-58937] - Human Placenta lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PSMB10/MECL1 Antibody (3J3G5) [NBP3-33276] - Western blot analysis of lysates from Mouse liver, using PSMB10/MECL1 Rabbit mAb at1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33276_rabbit-psmb10-mecl1-mab-3j3g5-9122024159580.jpg "Western Blot: PSMB10/MECL1 Antibody (3J3G5) [NBP3-33276] - Western blot analysis of lysates from Mouse liver, using PSMB10/MECL1 Rabbit mAb at1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.")
![Western Blot: PSMB10/MECL1 Antibody (3J3G5) [NBP3-33276] - Western blot analysis of various lysates using PSMB10/MECL1 Rabbit mAb at1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33276_rabbit-psmb10-mecl1-mab-3j3g5-912202414163042.jpg)
![Western Blot: PSMB10/MECL1 Antibody (S06-6C5) [NBP3-19751] - Western blot detection of PSMB10/MECL1 in C6 cell lysates using NBP3-19751 (1:500 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-S06-6C5-Western-Blot-NBP3-19751-img0001.jpg "Western Blot: PSMB10/MECL1 Antibody (S06-6C5) [NBP3-19751] - Western blot detection of PSMB10/MECL1 in C6 cell lysates using NBP3-19751 (1:500 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.")
![SDS-Page: Recombinant Human PSMB10/MECL1 His Protein [NBP1-72454] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-PSMB10-MECL1-His-Protein-SDS-Page-NBP1-72454-img0002.jpg "SDS-Page: Recombinant Human PSMB10/MECL1 His Protein [NBP1-72454] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
