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![Western Blot: Profilin 1 Antibody [NBP2-99325] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Western-Blot-NBP2-99325-img0002.jpg "Western Blot: Profilin 1 Antibody [NBP2-99325] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP2-99325] - Immunofluorescence staining of Profilin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Profilin 1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99325-img0001.jpg)
![Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.](http://images.novusbio.com/fullsize/nbp3-23345_rabbit-profilin-1-p-tyr129-pab-19920231856966.jpg "Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.")
![Western Blot: Profilin 1 Antibody (2Z1M4) [NBP3-16780] - Western blot analysis of extracts of various cell lines, using Profilin 1 Rabbit mAb (NBP3-16780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-2Z1M4-Western-Blot-NBP3-16780-img0001.jpg "Western Blot: Profilin 1 Antibody (2Z1M4) [NBP3-16780] - Western blot analysis of extracts of various cell lines, using Profilin 1 Rabbit mAb (NBP3-16780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![SDS-Page: Recombinant Human Profilin 1 Protein [NBP1-30215] - Profilin 1, 15.0 kDa (140aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Profilin-1-Protein-SDS-Page-NBP1-30215-img0001.jpg "SDS-Page: Recombinant Human Profilin 1 Protein [NBP1-30215] - Profilin 1, 15.0 kDa (140aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![ELISA: Human Profilin 1 ELISA Kit (Colorimetric) [NBP2-81243] - Samples were spiked with high concentrations of Human Profilin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81243_human-profilin-1-elisa-kit-colorimetric-132202416225274.jpg "ELISA: Human Profilin 1 ELISA Kit (Colorimetric) [NBP2-81243] - Samples were spiked with high concentrations of Human Profilin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Profilin 1 ELISA Kit (Colorimetric) [NBP2-81243] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81243_human-profilin-1-elisa-kit-colorimetric-432024955022.jpg)
![Western Blot: Profilin 1 Overexpression Lysate (Adult Normal) [NBL1-14318] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14318-img0002.jpg "Western Blot: Profilin 1 Overexpression Lysate (Adult Normal) [NBL1-14318] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Profilin 1.")
![SDS-Page: Profilin 1 Recombinant Protein [NBP2-52175] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Profilin-1-Recombinant-Protein-SDS-Page-NBP2-52175-img0001.jpg "SDS-Page: Profilin 1 Recombinant Protein [NBP2-52175] - 15% SDS Page (3 ug)")