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![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining in human cerebral cortex and liver tissues . Corresponding P2RY12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining in human cerebral cortex and liver tissues . Corresponding P2RY12 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: P2Y12/P2RY12 Antibody [NBP2-33870] - Quantitative biochemical measurements of P2Y12/P2RY12 protein and mRNA in human brains. Western blot measurements of P2Y12/P2RY12 levels in MTG samples from LP, HP and AD brains. Representative western blot image of P2Y12/P2RY12 polypeptide of MTG protein extracts identified with Novus antibody. Blots were normalized for levels of beta actin. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/21/2/678), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Western-Blot-NBP2-33870-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: P2Y12/P2RY12 Antibody [NBP2-33870] - Green-P2Y12/P2RY12, Blue-DAPI. 1:200 dilution for fixed tissue sections of feline optical nerve. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33870-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870] - Features of P2Y12/P2RY12-immunoreactive microglia. (I,J). Staining of representative sections with P2Y12/P2RY12 (Novus) antibody preabsorbed with immunizing peptide (I, +Pep) compared to staining of matched section with P2Y12/P2RY12 antibody non-absorbed (J, -Pep). (K,L). Staining of matched sections with alternative P2Y12/P2RY12 antibody. Same staining pattern of microglia revealed with P2Y12/P2RY12 (Novus) antibody (C) as with P2Y12/P2RY12 (Alomone Labs) antibody. All sections shown had been counterstained with neutral red to identify nuclei (red color). Abbreviations: ND: non-demented. AD: Alzheimer's disease. MTG: middle temporal gyrus.-Pep: antibody without immunizing peptide. + Pep: antibody with immunizing peptide. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/21/2/678), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-NBP2-33870-img0019.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebral cortex shows strong cytoplasmic positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0012.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0013.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0016.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebellum shows strong cytoplasmic positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0017.jpg)
![Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody [NBP2-33870] - Canine optic nerve. Immunofluorescent signal was detected using Alexa Fluor 488 conjugated secondary antibody (green). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Frozen-NBP2-33870-img0015.jpg)
![Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870] - Images demonstrating colocalization of CD105/MAB1097 with microglial markers (a) panels A, B Low magnification images of high-plaque (HP) case (panel A) & AD case (panel B) showing staining with CD105/MAB1097 (purple arrows) & IBA-1 (brown arrows) in MTG sections. Both images at same magnification: scale bars represent 100 μm. (b) (panel A) CD105 microglia (purple arrows) with ramified morphology stained with antibody to P2RY12 (brown arrow) in low-plaque (LP) case. (panel B) CD105 microglia (purple arrows) & HLA-DR (brown arrow) in AD case. (panel C) CD105 microglia (purple arrow) & CD45 (brown arrow) in AD case. All sections were from MTG. Images at same magnification: scale bars represent 50 μm. (c) (panel A–E). Patterns of staining of CD105 (purple) & IBA-1 (brown) in MTG sections of cases with progressively increasing pathology from LP to AD. (panel F). CD105-positive microglia in hippocampus (HPC) of AD case. Images at same magnification: scale bars represent 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31340569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33870_rabbit-p2y12-p2ry12-pab-310202416114763.jpg)