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![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Western-Blot-NBP2-00537-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NNMT Antibody (3D8) [NBP2-00537] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunocytochemistry-Immunofluorescence-NBP2-00537-img0008.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human prostate tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0007.jpg)
![Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Analysis of Jurkat cells, using anti-NNMT antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Flow-Cytometry-NBP2-00537-img0010.jpg)
![Western Blot: NNMT Antibody (3D8) [NBP2-00537] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Western-Blot-NBP2-00537-img0001.jpg)
![Western Blot: NNMT Antibody (3D8) [NBP2-00537] Analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Western-Blot-NBP2-00537-img0009.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0002.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0003.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human endometrium tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0004.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human liver tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0005.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human pancreas tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0006.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Western-Blot-NBP2-72984-img0003.jpg "Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.")
![Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human pancreas tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72984-img0010.jpg)
![Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72984-img0005.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Western-Blot-NBP2-72984-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Western-Blot-NBP2-72984-img0002.jpg)
![Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72984-img0006.jpg)
![Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72984-img0007.jpg)
![Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human endometrium tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72984-img0008.jpg)
![Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human liver tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72984-img0009.jpg)
![Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human prostate tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72984-img0011.jpg)
![Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Analysis of Jurkat cells, using anti-NNMT antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72984-img0004.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Analysis in human cell lines U2OS and SK-MEL-30 using anti-NNMT antibody. Corresponding NNMT RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Western-Blot-NBP2-32438-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Analysis in human cell lines U2OS and SK-MEL-30 using anti-NNMT antibody. Corresponding NNMT RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32438-img0010.jpg)
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunohistochemistry-Paraffin-NBP2-32438-img0020.jpg)
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunohistochemistry-Paraffin-NBP2-32438-img0017.jpg)
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunohistochemistry-Paraffin-NBP2-32438-img0018.jpg)
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human pancreas shows moderate to strong granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunohistochemistry-Paraffin-NBP2-32438-img0019.jpg)
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Nicotinamide N-Methyltransferase/NNMT Rabbit pAb (NBP3-03406) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunohistochemistry-Paraffin-NBP3-03406-img0003.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Nicotinamide N-Methyltransferase/NNMT Rabbit pAb (NBP3-03406) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] -analysis of A-549, using NNMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-03406_rabbit-polyclonal-nicotinamide-n-methyltransferase-nnmt-antibody-western-blot-273202410843..jpg)
![Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free [Nicotinamide N-Methyltransferase/NNMT] - Immunofluorescence analysis of paraffin-embedded human liver cancer using Nicotinamide N-Methyltransferase/NNMT Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03406_rabbit-polyclonal-nicotinamide-n-methyltransferase-nnmt-antibody-5220251628013.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP1-55398] - Reccomended Titration: 2.5 ug/ml Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Western-Blot-NBP1-55398-img0006.jpg "Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP1-55398] - Reccomended Titration: 2.5 ug/ml Positive Control: HepG2 cell lysate")
![Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP1-55398] - Human Adult Liver Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunohistochemistry-NBP1-55398-img0004.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP1-55398] - Antibody Titration: 0.2-1 ug/ml. A: - blocking peptide. B: + blocking peptide.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Western-Blot-NBP1-55398-img0003.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP1-55398] - Sample Tissue: Human Hela Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Western-Blot-NBP1-55398-img0005.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-AT11G11-Western-Blot-NBP2-50581-img0002.jpg "Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate")
![Immunocytochemistry/Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - ICC/IF analysis of NNMT in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-AT11G11-Immunocytochemistry-Immunofluorescence-NBP2-50581-img0005.jpg)
![Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - Flow cytometry analysis of NNMT in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-AT11G11-Flow-Cytometry-NBP2-50581-img0004.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2. Lane 1: NNMT transfected lysatE (29.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-2F2-Western-Blot-H00004837-M03-img0003.jpg "Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2. Lane 1: NNMT transfected lysatE (29.6 KDa). Lane 2: Non-transfected lysate.")
![ELISA: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Detection limit for recombinant GST tagged NNMT is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-2F2-ELISA-H00004837-M03-img0001.jpg)
![Immunoprecipitation: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Analysis of NNMT transfected lysate using anti-NNMT monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NNMT rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-2F2-Immunoprecipitation-H00004837-M03-img0002.jpg)
![Western Blot: NNMT Overexpression Lysate (Adult Normal) [NBL1-13700] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NNMT.](http://images.novusbio.com/fullsize/NNMT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13700-img0002.jpg "Western Blot: NNMT Overexpression Lysate (Adult Normal) [NBL1-13700] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NNMT.")
![SDS-Page: Recombinant Mouse Nicotinamide N-Methyltransferase/NNMT Protein [NBP2-59656] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Mouse-Nicotinamide-N-Methyltransferase-NNMT-Protein-SDS-Page-NBP2-59656-img0001.jpg "SDS-Page: Recombinant Mouse Nicotinamide N-Methyltransferase/NNMT Protein [NBP2-59656] - 15% SDS-PAGE (3ug)")
![ELISA: Human Nicotinamide N-Methyltransferase/NNMT ELISA Kit (Colorimetric) [NBP3-39164] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39164_human-nicotinamide-n-methyltransferase-nnmt-elisa-kit-2110202415295619.png "ELISA: Human Nicotinamide N-Methyltransferase/NNMT ELISA Kit (Colorimetric) [NBP3-39164] - Standard Curve Reference")
![ELISA: Human Nicotinamide N-Methyltransferase/NNMT - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39165] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39165_human-nicotinamide-n-methyltransferase-nnmt-r-t-u-elisa-kit-211020241536236.png "ELISA: Human Nicotinamide N-Methyltransferase/NNMT - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39165] - Standard Curve Reference")