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![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Lane 1: Wild-type HepG2 whole cell lysate (10 ug).Lane 2: CD206 knockdown HepG2 whole cell lysate (10 ug).Lane 3: CD206 knockdown HepG2 whole cell lysate (10 ug).NBP2-66956 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Lane 1: Wild-type HepG2 whole cell lysate (10 ug).Lane 2: CD206 knockdown HepG2 whole cell lysate (10 ug).Lane 3: CD206 knockdown HepG2 whole cell lysate (10 ug).NBP2-66956 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0003.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of MMR/CD206/Mannose Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: D3 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0005.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human lung tissue lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0006.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: 293T cell lysateLane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0002.jpg)
![Immunoprecipitation: MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Whole celllysate (1.0 mg per IP reaction; 20% of IP loaded) fromMUTZ-3 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-CD206/Mannose Receptorrecombinant monoclonal antibody used for IP at 6 ul per reaction. CD206/MannoseReceptor was also immunoprecipitated by rabbit antiCD206/Mannose Receptor antibody. Forblotting immunoprecipitated CD206/Mannose Receptor, was used at 1:1000. Chemiluminescence withan exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-Immunoprecipitation-NBP3-14698-img0001.jpg "Immunoprecipitation: MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Whole celllysate (1.0 mg per IP reaction; 20% of IP loaded) fromMUTZ-3 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-CD206/Mannose Receptorrecombinant monoclonal antibody used for IP at 6 ul per reaction. CD206/MannoseReceptor was also immunoprecipitated by rabbit antiCD206/Mannose Receptor antibody. Forblotting immunoprecipitated CD206/Mannose Receptor, was used at 1:1000. Chemiluminescence withan exposure time of 10 seconds.")
![MMR/CD206/Mannose Receptor Antibody (BLR109H)- Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit antiCD206/Mannose Receptor recombinant monoclonal antibody [BLR109H]used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody](http://images.novusbio.com/fullsize/nbp3-14698_rabbit-mmr-cd206-mannose-receptor-mab-western-blot-284202394352.jpg)
![MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Rabbit antiCD206/Mannose Receptor recombinant monoclonalantibody [BLR109H] . Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14698_rabbit-mmr-cd206-mannose-receptor-mab-immunohistochemistry-paraffin-284202394510..jpg)
![MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Rabbit antiCD206/Mannose Receptor recombinant monoclonalantibody [BLR109H] . Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14698_rabbit-mmr-cd206-mannose-receptor-mab-immunohistochemistry-paraffin-284202394646..jpg)
![Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - Frozen sections of human tonsil. NB600-1415 was used ina dilution of 1/25 and stains endothelia of lymph vesselsstrongly.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-15-2-Immunohistochemistry-Frozen-NB600-1415-img0003.jpg "Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - Frozen sections of human tonsil. NB600-1415 was used ina dilution of 1/25 and stains endothelia of lymph vesselsstrongly.")
![Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-15-2-Immunohistochemistry-Frozen-NB600-1415-img0002.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-15-2-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09101-img0002.jpg "Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Surface staining pattern of human human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-15-2-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09101-img0001.jpg)
![ELISA: Rat MMR/CD206/Mannose Receptor ELISA Kit (Colorimetric) [NBP3-08152] - Samples were spiked with high concentrations of Rat MMR/CD206/Mannose Receptor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08152_rat-mmr-cd206-mannose-receptor-elisa-kit-colorimetric-132202416211354.jpg "ELISA: Rat MMR/CD206/Mannose Receptor ELISA Kit (Colorimetric) [NBP3-08152] - Samples were spiked with high concentrations of Rat MMR/CD206/Mannose Receptor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat MMR/CD206/Mannose Receptor ELISA Kit (Colorimetric) [NBP3-08152] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08152_rat-mmr-cd206-mannose-receptor-elisa-kit-colorimetric-432024941576.jpg)
![ELISA: Human MMR/CD206/Mannose Receptor ELISA Kit (Colorimetric) [NBP3-08151] - Samples were spiked with high concentrations of Human MMR/CD206/Mannose Receptor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08151_human-mmr-cd206-mannose-receptor-elisa-kit-colorimetric-132202416102243.jpg "ELISA: Human MMR/CD206/Mannose Receptor ELISA Kit (Colorimetric) [NBP3-08151] - Samples were spiked with high concentrations of Human MMR/CD206/Mannose Receptor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human MMR/CD206/Mannose Receptor ELISA Kit (Colorimetric) [NBP3-08151] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08151_human-mmr-cd206-mannose-receptor-elisa-kit-colorimetric-4320248574343.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (S06-5I1) [NBP3-14900] - Western blot detection of MMR/CD206/Mannose Receptor in Jurkat, C6, Hela cell lysates using MMR/CD206/Mannose Receptor Rabbit mAb (1:1000 diluted). Predicted band size: 166kDa. Observed band size: 166kDa.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-S06-5I1-Western-Blot-NBP3-14900-img0001.jpg "Western Blot: MMR/CD206/Mannose Receptor Antibody (S06-5I1) [NBP3-14900] - Western blot detection of MMR/CD206/Mannose Receptor in Jurkat, C6, Hela cell lysates using MMR/CD206/Mannose Receptor Rabbit mAb (1:1000 diluted). Predicted band size: 166kDa. Observed band size: 166kDa.")