| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-168SS | |||
| Azide and BSA Free | NBP2-80847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3794-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3299-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72659 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21103 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3299-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21104 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68885-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58612-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26141-50ul | |||
![Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Western-Blot-NB100-168-img0002.jpg "Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.")
![Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT (Green) in Hela cells using NB100-168 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Immunocytochemistry-Immunofluorescence-NB100-168-img0004.jpg)
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 23.2) [NB100-168] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:2000 conc. of MGMT antibody (clone MT 23.2) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of MGMT was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Immunohistochemistry-Paraffin-NB100-168-img0013.jpg)
![Flow Cytometry: MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on HeLa cells with MGMT Antibody (MT 23.2) antibody NB100-168 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Cytometry-NB100-168-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MGMT (MT 23.2) NB100-168 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Immunocytochemistry-Immunofluorescence-NB100-168-img0006.jpg)
![Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Jurkat cells with MGMT Antibody (MT 23.2) antibody NB100-168 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Intracellular-NB100-168-img0008.jpg)
![Flow Cytometry: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in Human Jurkat Cell Line by Flow Cytometry. Human Jurkat cell line was stained with Mouse Anti- MGMT Monoclonal Antibody (Catalog # NB100-168, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Cytometry-NB100-168-img0011.jpg)
![Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Raji cells with MGMT Antibody (MT 23.2) antibody NB100-168AF647 (blue) and a matched isotype control NBP2-27231AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Intracellular-NB100-168-img0012.jpg)
![Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Jurkat cells with MGMT Antibody (MT 23.2) antibody NB100-168AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Intracellular-NB100-168-img0014.jpg)
![Simple Western: MGMT Antibody (MT 23.2) [NB100-168] - Simple Western lane view shows a specific band for MGMT in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Simple-Western-NB100-168-img0005.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Western-Blot-NB100-692-img0003.jpg "Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin fixed paraffin embedded human tonsil stained with MGMT antibody.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0002.jpg)
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MGMT MT 3.1 (Cat. #NB 100-692) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0001.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - MMR protein expression & hMutS alpha activity decreases while hprt mutation rates & MNNG resistance increases in MGMT & MMR negative subclones after repeated exposure to MNNG.A. MMR protein expression, each fluorescent protein band was measured against a loading control (p62) in the same lane by Alpha lnnotech Fluorochem HD2, histograms produced by Prism GraphPad software, error bars indicate SD. U251 not represented in histogram because hMSH6 & hMSH2 expression is completely absent (Figure 1). Asterisks (*) denote statistically significant differences at P < 0.05 between each subclone protein expression & HeLa MR for the designated MMR protein. Statistical significance determined by student t-test using Prism GraphPad software. Each experiment was performed a minimum of 3 times. B. hMutS alpha binding activity of equal nuclear protein concentration from nuclear extracts of each cell line by EMSA using [32P]-69mer oligomers with either G:T or 06meG:T located in the center. C. hprt mutation rates in two sequentially isolated HeLa MNNGR clones as compared to HeLa MR. D. Classic colony survival of HeLa MNNG4 & U251R subclones indicates significant resistance to 0.2 µM MNNG as compared to HeLa MR & U251 parental cell lines. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24019948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-692_mouse-monoclonal-mgmt-antibody-mt-3-1-31020241612636.jpg)
![Western Blot: MGMT Antibody (4F9) [NBP2-45843] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT.](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Western-Blot-NBP2-45843-img0018.jpg "Western Blot: MGMT Antibody (4F9) [NBP2-45843] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT.")
![Immunohistochemistry: MGMT Antibody (OTI4F9) [NBP2-45843] - MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-OTI4F9-Immunohistochemistry-NBP2-45843-img0019.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0001.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0002.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0003.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0004.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0005.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0006.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0007.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0008.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0009.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0010.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0012.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0013.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0014.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0015.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0016.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human tonsil within the normal limits using MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0017.jpg)
![ELISA: Human MGMT ELISA Kit (Colorimetric) [NBP2-60580] - Standard curve.](http://images.novusbio.com/fullsize/Human-MGMT-ELISA-Kit-Colorimetric-ELISA-NBP2-60580-img0001.jpg "ELISA: Human MGMT ELISA Kit (Colorimetric) [NBP2-60580] - Standard curve.")
![Western Blot: MGMT Antibody [NBP2-68885] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/MGMT-Antibody-Western-Blot-NBP2-68885-img0001.jpg "Western Blot: MGMT Antibody [NBP2-68885] - Analysis in human cell line RT-4.")
![Immunohistochemistry: MGMT Antibody [NBP2-68885] - Immunohistochemical staining of human testis shows nuclear positivity of varying intensity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MGMT-Antibody-Immunohistochemistry-NBP2-68885-img0002.jpg)
![Western Blot: MGMT Antibody [NBP2-58612] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/MGMT-Antibody-Western-Blot-NBP2-58612-img0002.jpg "Western Blot: MGMT Antibody [NBP2-58612] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: MGMT Antibody [NBP2-58612] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MGMT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58612-img0001.jpg)
![Immunohistochemistry: MGMT Antibody (10G7) [NBP3-26141] - Image of MGMT Antibody (10G7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26141_rabbit-mgmt-mab-10g7-2722024827497.jpg "Immunohistochemistry: MGMT Antibody (10G7) [NBP3-26141] - Image of MGMT Antibody (10G7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: MGMT Antibody (10G7) [NBP3-26141] - Positive Western Blot detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate. All lanes: MGMT Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/nbp3-26141_rabbit-mgmt-mab-10g7-282202411485842.jpg)