MERTK Products By Gene Name

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox"> AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." alt="J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_lightbox"> VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Mer knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Mer knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of U937 human histiocytic lymphoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for Mer at approximately 175 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Mer at approximately 175 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Mer was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse Mer Monoclonal Antibody (Catalog # MAB591) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAM1676) to detect phospho-Mer. Bands were visualized by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-Mer can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western blot." class="big_lightbox"> HAM1676) to detect phospho-Mer. Bands were visualized by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-Mer can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western blot." alt="NS0 cells transfected with human Mer (NS0-hMer) were treated with conditioned media from the human lung cell line, WI-38, for 15 minutes to induce tyrosine phosphorylation of Mer. Lysates were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-Mer monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with an HRP-conjugated anti-phosphotyrosine monoclonal antibody (R&D Systems, Catalog # HAM1676) to detect phospho-Mer. Bands were visualized by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-Mer can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western blot." class="big_thumb" />

BAF891). " class="big_lightbox"> BAF891). " class="big_thumb" />

891-MR), human Axl/Fc (R&D Systems, Catalog #154-AL), human Dtk/Fc (R&D Systems, Catalog #859-DK) or human TrkA/Fc (R&D Systems, Catalog #175-TK) were added to 100 μg lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human Mer. " class="big_lightbox"> 891-MR), human Axl/Fc (R&D Systems, Catalog #154-AL), human Dtk/Fc (R&D Systems, Catalog #859-DK) or human TrkA/Fc (R&D Systems, Catalog #175-TK) were added to 100 μg lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human Mer. " class="big_thumb" />