| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27825-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14118 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16941-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21086 | |||
![Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-27820241631683.jpg "Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32576) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-298202414262619.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunocytochemistry analysis of LOVO cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241114811.jpg)
![Flow Cytometry: MCM2 Antibody (PD00-90) [NBP3-32576] - Flow cytometric analysis of HL-60 cells labeling MCM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32576, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241637215.jpg)
![Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Mouse thymus tissue lysate (40 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: C2C12 cell lysate (20 ug/Lane) Lane 9: U-87 MG cell lysate (20 ug/Lane) Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-278202415212456.jpg "Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Mouse thymus tissue lysate (40 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: C2C12 cell lysate (20 ug/Lane) Lane 9: U-87 MG cell lysate (20 ug/Lane) Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-298202414262652.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunocytochemistry analysis of HeLa cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-29202410484867.jpg)
![Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887] - Western blot detection of MCM2 in Hela, A549, HL-60, U2OS cell lysates using NBP3-19887 (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.](http://images.novusbio.com/fullsize/MCM2-Antibody-S02-0D3-Western-Blot-NBP3-19887-img0003.jpg "Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887] - Western blot detection of MCM2 in Hela, A549, HL-60, U2OS cell lysates using NBP3-19887 (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.")
![Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunohistochemistry of MCM2 in paraffin-embedded Human tonsil using NBP3-19887 at dilution 1/50](http://images.novusbio.com/fullsize/MCM2-Antibody-S02-0D3-Immunohistochemistry-Paraffin-NBP3-19887-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunocytochemistry of MCM2 (green) in hela using NBP3-19887 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19887_rabbit-mcm2-mab-s02-0d3-113202515145446.jpg)
![Western Blot: MCM2 [p Ser 27] Antibody (SR1107) [NBP3-27825] - Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate.](http://images.novusbio.com/fullsize/nbp3-27825_rabbit-mcm2-p-ser-27-mab-sr1107-75202410133433.jpg "Western Blot: MCM2 [p Ser 27] Antibody (SR1107) [NBP3-27825] - Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate.")
![Western Blot: MCM2 Antibody (S08-9H4) [NBP3-14951] - Western blot detection of MCM2 in K562, C6, 3T3, Hela cell lysates using MCM2 Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.](http://images.novusbio.com/fullsize/MCM2-Antibody-S08-9H4-Western-Blot-NBP3-14951-img0001.jpg "Western Blot: MCM2 Antibody (S08-9H4) [NBP3-14951] - Western blot detection of MCM2 in K562, C6, 3T3, Hela cell lysates using MCM2 Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (S08-9H4) [NBP3-14951] - Immunofluorescence of MCM2 (green) in Hela cells using NBP3-14951 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14951_rabbit-mcm2-mab-s08-9h4-113202515145410.jpg)
![Western Blot: MCM2 [p Ser40] Antibody (S03-1B3) [NBP3-14952] - Western blot detection of MCM2 [p Ser40] in Hela, A549, MCF-7, 293T, C2C12 cell lysates using MCM2 [p Ser40] Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.](http://images.novusbio.com/fullsize/MCM2-p-Ser40-Antibody-S03-1B3-Western-Blot-NBP3-14952-img0001.jpg "Western Blot: MCM2 [p Ser40] Antibody (S03-1B3) [NBP3-14952] - Western blot detection of MCM2 [p Ser40] in Hela, A549, MCF-7, 293T, C2C12 cell lysates using MCM2 [p Ser40] Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.")
![Immunohistochemistry-Paraffin: MCM2 [p Ser40] Antibody (S03-1B3) [NBP3-14952] - Immunohistochemistry of MCM2 [p Ser40] in paraffin-embedded Human tonsil using MCM2 [p Ser40] Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/MCM2-p-Ser40-Antibody-S03-1B3-Immunohistochemistry-Paraffin-NBP3-14952-img0002.jpg)
![Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0058.jpg "Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MCM2 Antibody [NB100-244] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0057.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody (MCM2/3678) [NBP3-13965] - Formalin-fixed, paraffin-embedded human tonsil stained with MCM2 antibody (MCM2/3678). Inset: PBS instead of primary antibody, secondary only control.](http://images.novusbio.com/fullsize/MCM2-Antibody-MCM2-3678-Immunohistochemistry-Paraffin-NBP3-13965-img0001.jpg "Immunohistochemistry-Paraffin: MCM2 Antibody (MCM2/3678) [NBP3-13965] - Formalin-fixed, paraffin-embedded human tonsil stained with MCM2 antibody (MCM2/3678). Inset: PBS instead of primary antibody, secondary only control.")
![Immunohistochemistry-Paraffin: MCM2 Antibody (MCM2/3678) [NBP3-13965] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MCM2 antibody (MCM2/3678).](http://images.novusbio.com/fullsize/MCM2-Antibody-MCM2-3678-Immunohistochemistry-Paraffin-NBP3-13965-img0002.jpg)
![Western Blot: MCM2 Overexpression Lysate (Adult Normal) [NBL1-12950] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCM2.](http://images.novusbio.com/fullsize/MCM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12950-img0002.jpg "Western Blot: MCM2 Overexpression Lysate (Adult Normal) [NBL1-12950] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCM2.")
![Western Blot: MCM2 [p Ser139] Antibody [NBP3-16941] - Western blot analysis of extracts of HeLa cells, using MCM2 antibody (NBP3-16941) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37C for 1 hour.HeLa cells were treated by Serum-starvation overnight at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MCM2-[p-Ser139]-Antibody-Western-Blot-NBP3-16941-img0001.jpg "Western Blot: MCM2 [p Ser139] Antibody [NBP3-16941] - Western blot analysis of extracts of HeLa cells, using MCM2 antibody (NBP3-16941) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37C for 1 hour.HeLa cells were treated by Serum-starvation overnight at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
