| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82236-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto488 | NBP3-14785-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49603-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82247-0.025ml | |||
![Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239811.jpg "Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Mouse brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239936.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464548.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464554.jpg)
![Western Blot: Tau Antibody [NB100-82236] - Analysis of extracts from mouse brain.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NB100-82236-img0002.jpg "Western Blot: Tau Antibody [NB100-82236] - Analysis of extracts from mouse brain.")
![SDS-Page: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - DS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Preformed Fibrils (NBP2-76794). Lane 1: MW Ladder. Lane 2: Tau Protein Preformed Fibrils (NBP2-76794)](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--P301S-Mutant--Pre-formed-Fibrils-Protein-SDS-Page-NBP2-76794-img0001.jpg "SDS-Page: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - DS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Preformed Fibrils (NBP2-76794). Lane 1: MW Ladder. Lane 2: Tau Protein Preformed Fibrils (NBP2-76794)")
![In vitro assay: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) when tau Pre-formed Fibrils (NBP2-76794) are combined with tau monomers . The Pre-formed Fibrils seed the formation of new fibrils from a pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--P301S-Mutant--Pre-formed-Fibrils-Protein-In-vitro-assay-NBP2-76794-img0002.jpg)
![Electron Microscopy: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - TEM of recombinant Tau 441441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs). Fibrils were sonicated and stained with uranyl acetate.](http://images.novusbio.com/fullsize/Recombinant Human Tau 441 Active- P301S Mutant- Pre-formed Fibrils Protein-Electron Microscopy-NBP2-76794-img0004.jpg)
![Electron Microscopy: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - TEM of recombinant Tau 441441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV = 80kV. Fibrils were sonicated and stained with uranyl acetate.](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--P301S-Mutant--Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP2-76794-img0003.jpg)
![Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/Tau-[p-Ser262]-Antibody-Simple-Western-NB100-82242-img0004.jpg "Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.")
![Western Blot: Tau [p Ser262] Antibody [NB100-82242] - Western blot analysis of lysates from HeLa cells, using NB100-82242. The lane on the left is blocked with the phospho peptide.](http://images.novusbio.com/fullsize/nb100-82242_rabbit-polyclonal-tau-p-ser262-antibody-234202415453510.jpg)
![Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Western-Blot-NBP3-18265-img0002.jpg "Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Immunocytochemistry-Immunofluorescence-NBP3-18265-img0001.jpg)
![Dot Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Dot-Blot-NBP3-18265-img0003.jpg)
![Western Blot: Tau [p Thr217] Antibody [NBP3-13481] - Untreated (-) and treated (+) Neuro2A whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr217]-Antibody-Western-Blot-NBP3-13481-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tau [p Thr217] Antibody [NBP3-13481] - Untreated (-) and treated (+) Neuro2A whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>ELISA: Tau [p Thr217] Antibody [NBP3-13481] - Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (NBP3-13481) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr217]-Antibody-ELISA-NBP3-13481-img0002.jpg)
![IHC-P-Tau [p Thr217] Antibody-Rat brain. Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp3-13481_rabbit-tau-p-thr-217-pab-immunohistochemistry-paraffin-89202384342..jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-288202412461627.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (NBP3-32811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32811, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241492636.jpg)
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241420935.jpg)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591128..png "Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591256..png)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] -Staining of human fallopian tube shows negative positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591414..png)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591526..png)
![Western Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Western Blot analysis of Human Breast Cancer Cell line and Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/Tau-Antibody-1D5-Pre-formed-Fibrils-Western-Blot-NBP2-79825-img0004.jpg "Western Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Western Blot analysis of Human Breast Cancer Cell line and Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Dot Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.](http://images.novusbio.com/fullsize/Tau-Antibody-1D5-Pre-formed-Fibrils-Dot-Blot-NBP2-79825-img0005.jpg)
![Western Blot: Tau Antibody [NB100-82247] - Analysis of extracts from mouse brain tissue.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NB100-82247-img0005.jpg "Western Blot: Tau Antibody [NB100-82247] - Analysis of extracts from mouse brain tissue.")
![Immunohistochemistry-Paraffin: Tau Antibody [NB100-82247] - Analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using NB100-82247](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NB100-82247-img0003.jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-288202412461665.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32810) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32810) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-298202414161831.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-288202412461623.jpg "Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32808) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32808) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-298202414262664.jpg)