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![Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-288202412461654.jpg "Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32809) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-29820241492670.jpg)
![Western Blot: Tau Antibody [NBP3-18238] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.](http://images.novusbio.com/fullsize/Tau Antibody-Western Blot-NBP3-18238-img0002.jpg "Western Blot: Tau Antibody [NBP3-18238] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.")
![Immunohistochemistry: Tau Antibody [NBP3-18238] - Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Tissue: Brain Cortex. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238).](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NBP3-18238-img0001.jpg)
![Western Blot: Tau Antibody [NBP3-18237] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.](http://images.novusbio.com/fullsize/Tau Antibody-Western Blot-NBP3-18237-img0002.jpg "Western Blot: Tau Antibody [NBP3-18237] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.")
![Immunohistochemistry: Tau Antibody [NBP3-18237] - Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Tissue: Kidney. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237).](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NBP3-18237-img0001.jpg)
![Western Blot: Tau [p Ser416] Antibody [NB110-93503] - Rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Imunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/Tau-[p-Ser416]-Antibody-Western-Blot-NB110-93503-img0001.jpg "Western Blot: Tau [p Ser416] Antibody [NB110-93503] - Rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Imunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining in human cerebral cortex and liver tissues using anti-MAPT antibody. Corresponding MAPT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-Paraffin-NBP2-49607-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining in human cerebral cortex and liver tissues using anti-MAPT antibody. Corresponding MAPT RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-Paraffin-NBP2-49607-img0003.jpg)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-Paraffin-NBP2-49607-img0002.jpg)
![Western Blot: Tau [p Ser198] Antibody [NBP3-12927] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Ser198) antibody (NBP3-12927) diluted at 1:5000.](http://images.novusbio.com/fullsize/Tau-[p-Ser198]-Antibody-Western-Blot-NBP3-12927-img0002.jpg "Western Blot: Tau [p Ser198] Antibody [NBP3-12927] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Ser198) antibody (NBP3-12927) diluted at 1:5000.")
![<b>Biological Strategies Validation. </b>Western Blot: Tau [p Ser198] Antibody [NBP3-12927] - Untreated (-) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Ser198) antibody (NBP3-12927) diluted at 1:500.](http://images.novusbio.com/fullsize/Tau-[p-Ser198]-Antibody-Western-Blot-NBP3-12927-img0001.jpg)
![SDS-Page: Recombinant Human Tau 441 Active, Monomer, P301S Mutant Protein [NBP2-76795] - SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer (NBP2-76795). Lane 1: MW ladder. Lane 2: Tau Protein Monomer (NBP2-76795)](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--Monomer--P301S-Mutant-Protein-SDS-Page-NBP2-76795-img0001.jpg "SDS-Page: Recombinant Human Tau 441 Active, Monomer, P301S Mutant Protein [NBP2-76795] - SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer (NBP2-76795). Lane 1: MW ladder. Lane 2: Tau Protein Monomer (NBP2-76795)")
![In vitro assay: Recombinant Human Tau 441 Active, Monomer, P301S Mutant Protein [NBP2-76795] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (NBP2-76795). A greater increase in fluorescence is seen when 50 uM monomer (NBP2-76795) is combined with 10 nM PFFs , as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--Monomer--P301S-Mutant-Protein-In-vitro-assay-NBP2-76795-img0002.jpg)
![Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP3-11044-img0001.jpg "Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.")
![Dot Blot: Tau Antibody [NBP3-11044] - Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.](http://images.novusbio.com/fullsize/Tau-Antibody-Dot-Blot-NBP3-11044-img0002.jpg)
![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-13220241617342.jpg "ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-4320248582587.jpg)
![Human Tau [p 739] ELISA Kit (Colorimetric) - Standard Curve Reference](http://images.novusbio.com/fullsize/Human-Tau-p-739-ELISA-Kit-Colorimetric-img0001.jpg "Human Tau [p 739] ELISA Kit (Colorimetric) - Standard Curve Reference")
![ELISA: Human Tau [p Ser739] ELISA Kit (Colorimetric) [NBP2-66711] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66711_human-tau-p-ser739-elisa-kit-colorimetric-132202416143078.jpg)
![ELISA: Human Tau [p Ser739] ELISA Kit (Colorimetric) [NBP2-66711] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66711_human-tau-p-ser739-elisa-kit-colorimetric-432024955011.jpg)
![ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62749_human-tau-elisa-kit-colorimetric-13220241685316.jpg "ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62749_human-tau-elisa-kit-colorimetric-432024855036.jpg)
![Western Blot: Tau [p Thr205] Antibody - Azide and BSA Free [NBP3-16889] - Western blot analysis of lysates from Rat brain, using Tau Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-16889_rabbit-tau-p-thr-205-pab-52202511402761.jpg "Western Blot: Tau [p Thr205] Antibody - Azide and BSA Free [NBP3-16889] - Western blot analysis of lysates from Rat brain, using Tau Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Western Blot: Tau [p Thr205] Antibody - Azide and BSA Free [Tau] - Western blot analysis of lysates from Rat brain, using Tau Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-16889_rabbit-tau-p-thr-205-pab-52202517361298.jpg)