HAF008). For additional reference, Rec-ombinant Human Active ERK1 (Catalog #
1879-KS) and Recom-binant Human Active ERK2 (Catalog #
1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as in-dicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">
HAF008). For additional reference, Rec-ombinant Human Active ERK1 (Catalog #
1879-KS) and Recom-binant Human Active ERK2 (Catalog #
1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as in-dicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1940) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #
HAF008). For additional reference, Rec-ombinant Human Active ERK1 (Catalog #
1879-KS) and Recom-binant Human Active ERK2 (Catalog #
1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as in-dicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
HAF109). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1879) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
HAF008). For additional reference, Rec-ombinant Human Active ERK1 (Catalog # 1879-KS) and Recom-binant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as in-dicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1940) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Rec-ombinant Human Active ERK1 (Catalog # 1879-KS) and Recom-binant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as in-dicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-10122024140443.jpg "Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177017.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177023.jpg)
![Western Blot: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Western Blot analysis of various lysates using ERK1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit.<br>Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-31120251744429.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025173186.jpg)
![Western Blot: ERK1/2 Antibody [NBP3-05645] - Western blot analysis of extracts of various cell lines, using ERK1/2 antibody (NBP3-05645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NBP3-05645-img0003.jpg "Western Blot: ERK1/2 Antibody [NBP3-05645] - Western blot analysis of extracts of various cell lines, using ERK1/2 antibody (NBP3-05645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody - Azide and BSA Free [NBP3-05645] - Confocal immunofluorescence analysis of Hela cells using ERK1/2 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05645_rabbit-polyclonal-erk1-2-antibody-7220251245320.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP3-05645] - Immunofluorescence analysis of NIH-3T3 cells using ERK1/2 Polyclonal Antibody (NBP3-05645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05645-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP3-05645] - Confocal immunofluorescence analysis of Hela cells using ERK1/2 Polyclonal Antibody (NBP3-05645) at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05645-img0002.jpg)
![Western Blot: ERK1/2 Antibody [NBP3-05652] - Analysis of extracts of various cell lines, using ERK1/2 Rabbit pAb (NBP3-05652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NBP3-05652-img0004.jpg "Western Blot: ERK1/2 Antibody [NBP3-05652] - Analysis of extracts of various cell lines, using ERK1/2 Rabbit pAb (NBP3-05652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Rat lung using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Human urothelial carcinoma using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Mouse heart using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0002.jpg)
![Immunohistochemistry: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Image of ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26412_rabbit-erk1-erk2-p-thr-202-p-thr-185-mab-2d5-262202415475616.jpg "Immunohistochemistry: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Image of ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![<b>Biological Strategies Validation. </b>Western Blot: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Positive Western Blot detected in A549 whole cell lysate, HepG2 whole cell lysate (treated with EGF or not).<br /> All lanes: ERK1/ERK2 [p Thr202, p Thr185] Antibody at 2.35ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 42 KDa<br /> Observed band size: 42 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26412_rabbit-erk1-erk2-p-thr-202-p-thr-185-mab-2d5-282202411485841.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Staining of Hela cells with ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26412_rabbit-erk1-erk2-p-thr-202-p-thr-185-mab-2d5-282202412475142.jpg)
![ELISA ERK1 [Biotin]](http://images.novusbio.com/fullsize/dyc1940-2_human-total-erk1-duoset-ic-elisa-2-plate-elisa-1692020133745.jpg "ELISA ERK1 [Biotin]")
![Immunohistochemistry-Paraffin: ERK1 Antibody [NBP1-84807] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ERK1-Antibody-Immunohistochemistry-Paraffin-NBP1-84807-img0002.jpg "Immunohistochemistry-Paraffin: ERK1 Antibody [NBP1-84807] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.")
![Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-84807_rabbit-polyclonal-erk1-antibody-10420251543582.jpg)
![Immunohistochemistry-Paraffin: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Immunochemical staining of human ERK1/ERK21 in human breast carcinoma with NBP3-23530 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23530_rabbit-erk1-erk2-pab-azide-and-bsa-free-12620241540464.png "Immunohistochemistry-Paraffin: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Immunochemical staining of human ERK1/ERK21 in human breast carcinoma with NBP3-23530 at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Immunochemical staining of human ERK1/ERK2 in human lung cancer with NBP3-23530 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23530_rabbit-erk1-erk2-pab-azide-and-bsa-free-136202422345083.png)
![<b>Biological Strategies Validation. </b>Western Blot: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100nM, 30 min; +), using NBP3-23530 at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-23530_rabbit-erk1-erk2-pab-azide-and-bsa-free-136202422345065.png)
![Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Western-Blot-NBP1-74032-img0007.jpg "Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1A2) [NBP1-74032] - Analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Immunocytochemistry-Immunofluorescence-NBP1-74032-img0008.jpg)
![Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with antihuman MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MAPK1 recombinant protein Lane 2.: MAPK3 recombinant protein](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Western-Blot-NBP1-74032-img0005.jpg)
![Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Western-Blot-NBP1-30128-img0001.jpg "Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (B20-U) [NBP1-30128] - Expression in HEK293 cells.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Immunocytochemistry-Immunofluorescence-NBP1-30128-img0005.jpg)
