| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05652-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1879-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1940-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26412-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21670-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74032-50ul | |||
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-10122024140443.jpg "Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177017.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177023.jpg)
![Western Blot: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Western Blot analysis of various lysates using ERK1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-31120251744429.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025173186.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)
![Western Blot: ERK1/2 Antibody [NBP3-05645] - Western blot analysis of extracts of various cell lines, using ERK1/2 antibody (NBP3-05645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NBP3-05645-img0003.jpg "Western Blot: ERK1/2 Antibody [NBP3-05645] - Western blot analysis of extracts of various cell lines, using ERK1/2 antibody (NBP3-05645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody - Azide and BSA Free [NBP3-05645] - Confocal immunofluorescence analysis of Hela cells using ERK1/2 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05645_rabbit-polyclonal-erk1-2-antibody-7220251245320.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP3-05645] - Immunofluorescence analysis of NIH-3T3 cells using ERK1/2 Polyclonal Antibody (NBP3-05645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05645-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP3-05645] - Confocal immunofluorescence analysis of Hela cells using ERK1/2 Polyclonal Antibody (NBP3-05645) at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05645-img0002.jpg)
![Western Blot: ERK1/2 Antibody [NBP3-05652] - Analysis of extracts of various cell lines, using ERK1/2 Rabbit pAb (NBP3-05652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NBP3-05652-img0004.jpg "Western Blot: ERK1/2 Antibody [NBP3-05652] - Analysis of extracts of various cell lines, using ERK1/2 Rabbit pAb (NBP3-05652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Rat lung using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Human urothelial carcinoma using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Mouse heart using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0002.jpg)
![ELISA ERK1 [Biotin]](http://images.novusbio.com/fullsize2/dyc1940-2_human-total-erk1-duoset-ic-elisa-2-plate-elisa-1692020133745.jpg "ELISA ERK1 [Biotin]")
![Immunohistochemistry-Paraffin: ERK1 Antibody [NBP1-84807] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ERK1-Antibody-Immunohistochemistry-Paraffin-NBP1-84807-img0002.jpg "Immunohistochemistry-Paraffin: ERK1 Antibody [NBP1-84807] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.")
![Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-84807_rabbit-polyclonal-erk1-antibody-10420251543582.jpg)
![Immunohistochemistry: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Image of ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26412_rabbit-erk1-erk2-p-thr-202-p-thr-185-mab-2d5-262202415475616.jpg "Immunohistochemistry: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Image of ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Positive Western Blot detected in A549 whole cell lysate, HepG2 whole cell lysate (treated with EGF or not). All lanes: ERK1/ERK2 [p Thr202, p Thr185] Antibody at 2.35ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42 KDa Observed band size: 42 KDa](http://images.novusbio.com/fullsize/nbp3-26412_rabbit-erk1-erk2-p-thr-202-p-thr-185-mab-2d5-282202411485841.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Staining of Hela cells with ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26412_rabbit-erk1-erk2-p-thr-202-p-thr-185-mab-2d5-282202412475142.jpg)
![Western Blot: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Western blot analysis using NBP3-21670 in HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/nbp3-21670_rabbit-erk1-erk2-mab-sr1700-5720231624729.jpg "Western Blot: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Western blot analysis using NBP3-21670 in HepG2 whole cell lysates.")
![Immunocytochemistry/Immunofluorescence: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Immunofluorescent analysis of Hela cells, using NBP3-21670.](http://images.novusbio.com/fullsize/nbp3-21670_rabbit-erk1-erk2-mab-sr1700-57202315521818.jpg)
![Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Western-Blot-NBP1-74032-img0007.jpg "Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1A2) [NBP1-74032] - Analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Immunocytochemistry-Immunofluorescence-NBP1-74032-img0008.jpg)
![Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with antihuman MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MAPK1 recombinant protein Lane 2.: MAPK3 recombinant protein](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Western-Blot-NBP1-74032-img0005.jpg)