| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2319-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | MAB23191-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-63601-0.025mg | |||
![Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-278202416285213.jpg "Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-2920241192322.jpg)
![Western Blot: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32514) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32514_rabbit-lag-3-mab-psh0-43-278202416331289.jpg "Western Blot: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32514) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32514) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32514_rabbit-lag-3-mab-psh0-43-298202414115425.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09044-img0002.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![ELISA: LAG-3 Antibody (C9B7W) - Chimeric - Azide and BSA Free [NBP3-09044] - ELISA Plate coated with recombinant mouse LAG-3 Fc-Fusion Protein at a concentration of 2 ug/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using NBP3-09044. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/nbp3-09044_rabbit-monoclonal-lag-3-antibody-c9b7w-chimeric-74202522113011.png)
![ELISA: Human LAG-3 ELISA Kit (Colorimetric) [NBP3-06915] - Samples were spiked with high concentrations of Human LAG-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06915_human-lag-3-elisa-kit-colorimetric-132202416121322.jpg "ELISA: Human LAG-3 ELISA Kit (Colorimetric) [NBP3-06915] - Samples were spiked with high concentrations of Human LAG-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human LAG-3 ELISA Kit (Colorimetric) [NBP3-06915] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06915_human-lag-3-elisa-kit-colorimetric-432024963957.jpg)
![Western Blot: LAG-3 Overexpression Lysate [NBP2-04223] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lymphocyte Activation Gene 3 (observed molecular weight of ~ 57 KDa).](http://images.novusbio.com/fullsize/Lymphocyte-Activation-Gene-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04223-img0001.jpg "Western Blot: LAG-3 Overexpression Lysate [NBP2-04223] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lymphocyte Activation Gene 3 (observed molecular weight of ~ 57 KDa).")
![Flow Cytometry: LAG-3 Antibody (C9B7W) [NB100-63601] - Staining of CD223 transfected cells with RAT ANTI MOUSE CD223](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Flow-Cytometry-NB100-63601-img0002.jpg "Flow Cytometry: LAG-3 Antibody (C9B7W) [NB100-63601] - Staining of CD223 transfected cells with RAT ANTI MOUSE CD223")