| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-23442-25ug | |||
| BSA Free | NBP3-23442-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71112 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26839-25ul | |||
| Azide and BSA Free | NBP3-44285 | |||
![Western Blot: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Western blot analysis of LAG-3 mAb (L4-PL33): Lane 1: MW marker, Lane 2: LAG-3 (human): Fc (human), (recombinant).](http://images.novusbio.com/fullsize/LAG-3-Antibody-L4-PL33-Azide-Free-Western-Blot-NBP3-05777-img0001.jpg "Western Blot: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Western blot analysis of LAG-3 mAb (L4-PL33): Lane 1: MW marker, Lane 2: LAG-3 (human): Fc (human), (recombinant).")
![Immunohistochemistry-Paraffin: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tonsil tissue stained with LAG-3 (human) recombinant mAb (L4-PL33) at 6ug/ml.](http://images.novusbio.com/fullsize/LAG-3-Antibody-L4-PL33-Azide-Free-Immunohistochemistry-Paraffin-NBP3-05777-img0002.jpg)
![ELISA: Human LAG-3 ELISA Kit (Colorimetric) [NBP3-06915] - Samples were spiked with high concentrations of Human LAG-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06915_human-lag-3-elisa-kit-colorimetric-132202416121322.jpg "ELISA: Human LAG-3 ELISA Kit (Colorimetric) [NBP3-06915] - Samples were spiked with high concentrations of Human LAG-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human LAG-3 ELISA Kit (Colorimetric) [NBP3-06915] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06915_human-lag-3-elisa-kit-colorimetric-432024963957.jpg)
![Western Blot: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3.](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Western-Blot-NBP2-45581-img0004.jpg "Western Blot: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3.")
![Immunohistochemistry: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Immunohistochemistry-NBP2-45581-img0002.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-LAG3 antibody (1:100).](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Flow-Cytometry-NBP2-45581-img0005.jpg)
![Immunohistochemistry: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Immunohistochemistry-NBP2-45581-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (1A6) - VHH - Azide and BSA Free [NBP3-12837] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp3-12837_llama-monoclonal-lag-3-antibody-1a6-vhh-271120241958496.gif "Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (1A6) - VHH - Azide and BSA Free [NBP3-12837] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.")
![Flow Cytometry: LAG-3 Antibody (1A6) - VHH [NBP3-12837] - Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1A6-VHH-Flow-Cytometry-NBP3-12837-img0003.jpg)
![ELISA: LAG-3 Antibody (1A6) - VHH [NBP3-12837] - Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1A6-VHH-ELISA-NBP3-12837-img0001.jpg)
![Immunohistochemistry: LAG-3 Antibody (1A6) - VHH - Azide and BSA Free [NBP3-12837] - Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp3-12837_llama-monoclonal-lag-3-antibody-1a6-vhh-2711202419562161.gif)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12836-img0004.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.")
![Immunohistochemistry: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-Immunohistochemistry-NBP3-12836-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-Flow-Cytometry-NBP3-12836-img0003.jpg)
![ELISA: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-ELISA-NBP3-12836-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12838-img0004.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.")
![Immunohistochemistry: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-Immunohistochemistry-NBP3-12838-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-Flow-Cytometry-NBP3-12838-img0003.jpg)
![ELISA: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-ELISA-NBP3-12838-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12839-img0004.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.")
![Immunohistochemistry: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-Immunohistochemistry-NBP3-12839-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-Flow-Cytometry-NBP3-12839-img0003.jpg)
![ELISA: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-ELISA-NBP3-12839-img0001.jpg)
![Western Blot: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32515) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-278202415212421.jpg "Western Blot: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32515) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32515) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-29820241435254.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-2920241033400.jpg)
![Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-2882024142261.jpg "Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32516) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29820241416185.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG-3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29202410334064.jpg)