285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMP2/PSMB9 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMP2/PSMB9 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line treated (+) with 100 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMP2/PSMB9 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

285-IF) for 24 hours using Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> 285-IF) for 24 hours using Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />