| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-529SS | |||
443-KD) inhibits Recombinant Mouse VEGF164(Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164(5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (150 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB4431). The ND50 is typically 0.05-0.15 µg/mL." alt="Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164(Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164(5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (150 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB4431). The ND50 is typically 0.05-0.15 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4431-SP | |||
| Carrier Free | MAB4431-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 443-KD-050/CF | |||
357-KD) was serially diluted and captured by Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3573) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD) was serially diluted and captured by Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3573) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3573-SP | |||
| Carrier Free | MAB3573-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB443-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-18644-0.02mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05772SS | |||
MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113)." alt="bEnd.3 mouse endo-thelioma cell line was stained with Rat Anti-Mouse VEGFR2/KDR/Flk-1 Mono-clonal Antibody (Catalog # MAB4432, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-18646-0.02mg | |||
![Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet in VEGFA induced HUVEC cell lysate (50 ug) using NB100-529 (4 ug/mL). ECL detection 2.5 minutes.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-Western-Blot-NB100-529-img0010.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet in VEGFA induced HUVEC cell lysate (50 ug) using NB100-529 (4 ug/mL). ECL detection 2.5 minutes.")
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - The VEGF R2/KDR/Flk-1antibody was tested in V6.5 mouse embryonic stem cells (NBP1-41162) with DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained with DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-529-img0008.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF Receptor 2 chimera transfected lysate (20 ug) using NB100-529 (6 ug/mL). ECL detection 30 seconds.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-Western-Blot-NB100-529-img0009.jpg)
![N/A VEGFR2/KDR/Flk-1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_VEGF_R2_DY357_ELISA_1959.jpg "N/A VEGFR2/KDR/Flk-1 [Biotin]")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644] - VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit antihuman KDR (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-Immunocytochemistry-Immunofluorescence-NBP1-18644-img0017.jpg "Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644] - VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit antihuman KDR (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE.")
![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Analysis using the Biotin conjugate of NBP1-18644. Staining of VEGFR-2/KDR expression in HUVE cells.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0011.jpg)
![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Fig. 1: Flow analysis of VEGF R2/KDR/Flk-1 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC).](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0008.jpg)
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - 1 Sandwich-ELISA using soluble KDR (D7) as standard. Mouse anti-human VEGFR-2 was used as capture antibody, Biotinylated rabbit anti-human VEGFR-2 was used for detection.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0006.jpg)
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Total lysate from PAE/KDR cells was measured for expression of VEGFR- 1/KDR by a standard Sandwich ELISA using a mouse monoclonal anti-human VEGFR-1 antibody for capturing and a Biotin conjugated rabbit polyclonal anti-human VEGFR-1 antibody for detection. Total lysate from PAE/Flt-1 and PAE/FLT-4 was used as negative control. As positive control, total lysate from PAE/Flt-1 was spiked with recombinant human sKDR.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0018.jpg)
![ELISA VEGFR2/KDR/Flk-1 [Biotin]](http://images.novusbio.com/fullsize/dyc1780-2_human-total-vegf-r2-kdr-duoset-ic-elisa-5-plate-elisa-16920209736.jpg "ELISA VEGFR2/KDR/Flk-1 [Biotin]")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NBP3-05772] - HepG2 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-VEGFR2/KDR at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05772-img0002.jpg "Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NBP3-05772] - HepG2 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-VEGFR2/KDR at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody [NBP3-05772] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of VEGFR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05772-img0001.jpg)
NL013) and counterstained with DAPI (blue). View our protocol for ![Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Recombinant human endogenous soluble VEGFR-2/KDR (esKDR) was produced in insect cells. Western blot was performed using our monoclonal anti-VEGFR-2 recognizing the soluble as well as the transmembrane form of KDR (left panal) and our new poyclonal antibody directed against the unique C-terminal end of the endogenous sKDR (CGRETILDHSAEAVGMP) recognizing solely the endogenous form but not sKDR (D7) and sKDR (D7)-Fc consisting of the full extraplasmatic domain. The endogenous sKDR generated by alternative splicing consists of the first 6 Ig-like loops with a unique c-terminal end.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-20I6-Western-Blot-NBP1-18646-img0006.jpg "Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Recombinant human endogenous soluble VEGFR-2/KDR (esKDR) was produced in insect cells. Western blot was performed using our monoclonal anti-VEGFR-2 recognizing the soluble as well as the transmembrane form of KDR (left panal) and our new poyclonal antibody directed against the unique C-terminal end of the endogenous sKDR (CGRETILDHSAEAVGMP) recognizing solely the endogenous form but not sKDR (D7) and sKDR (D7)-Fc consisting of the full extraplasmatic domain. The endogenous sKDR generated by alternative splicing consists of the first 6 Ig-like loops with a unique c-terminal end.")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - HMEC-1 CRL-3243 cells, stained with 1:50 VEGFR2/KDR/Flk-1 antibody (NBP1-18645AF488) (green) and DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-20I6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-18646-img0016.jpg)
![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Analysis with primary human dermal lymphatic endothelial cells(HDLEC).](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-20I6-Flow-Cytometry-NBP1-18646-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Staining (green) of VEGFR-2 (KDR) in primary human umbilical vein endothelial cells (HUVEC) with anti-human VEGFR-2 (Cl.4) (7,5ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:600.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-20I6-Immunocytochemistry-Immunofluorescence-NBP1-18646-img0015.jpg)
