| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47984-0.1mg | |||
| IHC-Prediluted | NBP2-48048 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44944 | |||
| Azide and BSA Free | NBP2-34404-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44944 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47978-0.1mg | |||
| IHC-Prediluted | NBP2-48076 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08634 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48066 | |||
| Azide and BSA Free | NBP3-11544 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48085 | |||
| Azide and BSA Free | NBP3-11545 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34414-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90353-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48085 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48055 | |||
![Western Blot: Cytokeratin 18 Antibody (KRT18/1190) [NBP2-44942] - Western Blot Analysis of human A549 and HEP-2 cell lysate using Cytokeratin 18 Antibody (KRT18/1190).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-1190-Western-Blot-NBP2-44942-img0006.jpg "Western Blot: Cytokeratin 18 Antibody (KRT18/1190) [NBP2-44942] - Western Blot Analysis of human A549 and HEP-2 cell lysate using Cytokeratin 18 Antibody (KRT18/1190).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/1190) [NBP2-44942] - Confocal immunofluorescence image of HeLa cells using Cytokeratin 18 Antibody (KRT18/1190). Green (CF488). Red Dot is used to label nuclei Red.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-1190-Immunocytochemistry-Immunofluorescence-NBP2-44942-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/1190) [NBP2-44942] - Rat Colon stained with Cytokeratin 18 Monoclonal Antibody (KRT18/1190).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-1190-Immunohistochemistry-Paraffin-NBP2-44942-img0002.jpg)
![Western Blot: Cytokeratin 18 Antibody (KRT18/1190) [NBP2-44942] - Western Blot Analysis of human A) Stomach B) Intestine lysate using Cytokeratin 18 Antibody (KRT18/1190).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-1190-Western-Blot-NBP2-44942-img0004.jpg)
![Western Blot: Cytokeratin 18 Antibody (KRT18/1190) [NBP2-44942] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/1190).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-1190-Western-Blot-NBP2-44942-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/1190) [NBP2-44942] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/1190).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-1190-Immunohistochemistry-Paraffin-NBP2-44942-img0001.jpg)
![Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (SPM265).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Western-Blot-NBP2-34356-img0004.jpg "Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (SPM265).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Confocal Immunofluorescence of HeLa cells Cytokeratin 18 Antibody (SPM265). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Immunocytochemistry-Immunofluorescence-NBP2-34356-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Formalin-paraffin skin sweat gland stained with Cytokeratin 18 MAb (SPM265). Note cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Immunohistochemistry-Paraffin-NBP2-34356-img0001.jpg)
![Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Western Blot 1) HeLa and 2) A431 Lysate using Cytokeratin 18 Monoclonal Antibody (SPM265)](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Western-Blot-NBP2-34356-img0002.jpg)
![Western Blot: Cytokeratin 18 Antibody (SPM265) - Azide and BSA Free [NBP2-34404] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (SPM265).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Azide-and-BSA-Free-Western-Blot-NBP2-34404-img0003.jpg "Western Blot: Cytokeratin 18 Antibody (SPM265) - Azide and BSA Free [NBP2-34404] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (SPM265).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SPM265) - Azide and BSA Free [NBP2-34404] - Confocal Immunofluorescence of HeLa cells Cytokeratin 18 Antibody (SPM265). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34404-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM265) - Azide and BSA Free [NBP2-34404] - Formalin-paraffin skin sweat gland stained with Cytokeratin 18 MAb (SPM265). Note cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34404-img0004.jpg)
![Western Blot: Cytokeratin 18 Antibody (SPM265) - Azide and BSA Free [NBP2-34404] - Western Blot 1) HeLa and 2) A431 Lysate using Cytokeratin 18 Monoclonal Antibody (SPM265)](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM265-Azide-and-BSA-Free-Western-Blot-NBP2-34404-img0002.jpg)
![Western Blot: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (Cocktail). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Cocktail-Western-Blot-NBP2-44907-img0007.jpg "Western Blot: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (Cocktail). Image using the Azide and BSA Free format of this antibody.")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Cocktail-Immunohistochemistry-Paraffin-NBP2-44907-img0004.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 18 Antibody (Cocktail) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Cocktail-Flow-Cytometry-NBP2-44907-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - Human Cervical Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Cocktail-Immunohistochemistry-Paraffin-NBP2-44907-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Cocktail-Immunohistochemistry-Paraffin-NBP2-44907-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - Human Lung Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Cocktail-Immunohistochemistry-Paraffin-NBP2-44907-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) [NBP2-44907] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Cocktail-Immunohistochemistry-Paraffin-NBP2-44907-img0005.jpg)
![Western Blot: Cytokeratin 18 Antibody (KRT18/2808R) [NBP3-07692] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-2808R-Western-Blot-NBP3-07692-img0006.jpg "Western Blot: Cytokeratin 18 Antibody (KRT18/2808R) [NBP3-07692] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/2808R) [NBP3-07692] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 18 with Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-2808R-Immunocytochemistry-Immunofluorescence-NBP3-07692-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/2808R) [NBP3-07692] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-2808R-Immunohistochemistry-Paraffin-NBP3-07692-img0005.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (KRT18/2808R) [NBP3-07692] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-2808R-Flow-Cytometry-NBP3-07692-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/2808R) [NBP3-07692] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-2808R-Immunohistochemistry-Paraffin-NBP3-07692-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/2808R) [NBP3-07692] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-2808R-Immunohistochemistry-Paraffin-NBP3-07692-img0004.jpg)
![Western Blot: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/836)](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-836-Western-Blot-NBP2-44950-img0004.jpg "Western Blot: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/836)")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Confocal Immunofluorescence image of HeLa cells using Cytokeratin 18 Antibody (KRT18/836) Green (CF488) and Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-836-Immunocytochemistry-Immunofluorescence-NBP2-44950-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/836).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-836-Immunohistochemistry-Paraffin-NBP2-44950-img0001.jpg)
![Protein Array: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Analysis of Protein Array containing >19,000 full-length human proteins using Cytokeratin 18 Antibody (KRT18/836) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-836-Protein-Array-NBP2-44950-img0003.jpg)
![Western Blot: Cytokeratin 18 Antibody (B23.1) [NBP2-44952] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Monoclonal Antibody (B23.1).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-B23-1-Western-Blot-NBP2-44952-img0003.jpg "Western Blot: Cytokeratin 18 Antibody (B23.1) [NBP2-44952] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Monoclonal Antibody (B23.1).")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (B23.1) [NBP2-44952] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (B23.1).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-B23-1-Immunohistochemistry-Paraffin-NBP2-44952-img0001.jpg)
![Western Blot: Cytokeratin 18 Antibody (B23.1) [NBP2-44952] - Western Blot analysis of human stomach lysate using Cytokeratin 18 Monoclonal Antibody (B23.1).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-B23-1-Western-Blot-NBP2-44952-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM510) [NBP2-34367] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (SPM510).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM510-Immunohistochemistry-Paraffin-NBP2-34367-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM510) [NBP2-34367] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (SPM510).")
![Flow Cytometry: Cytokeratin 18 Antibody (SPM510) [NBP2-34367] - Flow Cytometric Analysis of trypsinized paraformaldehyde-fixed HeLa cells using Cytokeratin 18 Antibody (SPM510) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SPM510-Flow-Cytometry-NBP2-34367-img0002.jpg)
![Western Blot: Cytokeratin 18 Antibody [NBP1-90353] - Analysis in human cell lines MCF-7 and SK-MEL-30 using anti-KRT18 antibody. Corresponding KRT18 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Western-Blot-NBP1-90353-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cytokeratin 18 Antibody [NBP1-90353] - Analysis in human cell lines MCF-7 and SK-MEL-30 using anti-KRT18 antibody. Corresponding KRT18 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90353-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human small intestine shows moderate cytoplasmic and membrane positivity in glandular cells.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Immunohistochemistry-Paraffin-NBP1-90353-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human kidney shows moderate cytoplasmic and membrane positivity in cells in tubules.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Immunohistochemistry-Paraffin-NBP1-90353-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human placenta shows moderate cytoplasmic and membrane positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Immunohistochemistry-Paraffin-NBP1-90353-img0013.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-Immunohistochemistry-Paraffin-NBP1-90353-img0014.jpg)
![Western Blot: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A431 cell lysate Lane 4: HT-29 cell lysate Lane 5: HepG2 cell lysate Lane 6: HCT 116 cell lysate Lane 7: Huh7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32246_mouse-cytokeratin-18-mab-6-19-r-27820241521243.jpg "Western Blot: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A431 cell lysate Lane 4: HT-29 cell lysate Lane 5: HepG2 cell lysate Lane 6: HCT 116 cell lysate Lane 7: Huh7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32246) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32246_mouse-cytokeratin-18-mab-6-19-r-298202414333667.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 18 with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32246_mouse-cytokeratin-18-mab-6-19-r-29202410484865.jpg)