| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89446-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86002-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86416-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05100-20ul | |||
![Western Blot: KIF2B Antibody [NBP1-89446] - Analysis in control (vector only transfected HEK293T lysate) and KIF2B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/KIF2B-Antibody-Western-Blot-NBP1-89446-img0008.jpg "Western Blot: KIF2B Antibody [NBP1-89446] - Analysis in control (vector only transfected HEK293T lysate) and KIF2B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: KIF2B Antibody [NBP1-89446] - Staining of human cell line U-2 OS shows localization to nucleoli, microtubules, cytokinetic bridge & mitotic spindle. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KIF2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89446-img0007.jpg)
![Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-89446] - Staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/KIF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89446-img0005.jpg)
![Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-86002] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/KIF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-86002-img0001.jpg "Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-86002] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in seminiferous ducts.")
![Western Blot: KIF2B Antibody [NBP1-86002] - TBK1 depletion alters microtubule dynamics.(a) Shcontrol A549 or H1650 cells treated with 0.1 μM nocodazole for 15 min showed complete disruption of microtubules, while TBK1-depleted shTBK1 A549 or H1650 cells retained stable microtubules as seen by staining for alpha tubulin (green) & CEP170 (red). (b) Mitotic spindles in TBK1-depleted A549 & H460 cells were more stable after nocodazole treatment as compared to control cells. Alpha tubulin (green) shows mitotic spindles. (c) shTBK1 A549 or H1650 cells when subjected to cold temperature (4 °C) & subsequent incubation at warm temperature (37 °C) had stable microtubules versus analogously treated shcontrol A549 or H1650 cells. Alpha Tubulin (green), CEP170 (red) & DNA (DAPI, Blue). (d) Similar results were observed in mitotic cells where mitotic spindles of TBK1-depleted A549 or H460 cells were more stable compared to control shRNA-treated cells. Alpha tubulin (green). (e) Stability of microtubules were drastically reduced in A549 cells overexpressing TBK1 when treated with 5 μM nocodazole & allowed to regrow for 7 min; Scale bar, 10 μm for e. (f) Low magnification images of cells in c. Scale bar, 25 μm. (g–i) TBK1 is required for the binding of CEP170 with Kif2b (g) as well as for the association of NuMA with dynein (i), as seen by immunoprecipitation–western blot analysis. (h) Western blot for pH3S10 to confirm mitotic fractions. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms10072), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-86002_rabbit-polyclonal-kif2b-antibody-310202416114729.jpg)
![Western Blot: KIF2B Antibody [NBP1-86416] - Analysis in control (vector only transfected HEK293T lysate) and KIF2B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/KIF2B-Antibody-Western-Blot-NBP1-86416-img0005.jpg "Western Blot: KIF2B Antibody [NBP1-86416] - Analysis in control (vector only transfected HEK293T lysate) and KIF2B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-86416] - Staining of human testis shows moderate cytoplasmic and nuclear postivity in seminiferous ducts.](http://images.novusbio.com/fullsize/KIF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-86416-img0006.jpg)
![Western Blot: KIF2B Antibody - BSA Free [NBP3-46392] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (KIF2B antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-46392_rabbit-kif2b-pab-bsa-free-284202511543990.jpg "Western Blot: KIF2B Antibody - BSA Free [NBP3-46392] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (KIF2B antibody) at dilution of 1:1000")
![Immunoprecipitation: KIF2B Antibody - BSA Free [NBP3-46392] - IP Result of anti-KIF2B (IP:, 4ug; Detection: 1:1000) with mouse testis tissue lysate 4000ug.](http://images.novusbio.com/fullsize/nbp3-46392_rabbit-kif2b-pab-bsa-free-284202511541870.jpg)
![Western Blot: KIF2B Antibody - BSA Free [NBP3-05100] - Western blot analysis of various lysates, using KIF2B Rabbit pAb (A6480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-05100_rabbit-polyclonal-kif2b-antibody-52202519302943.jpg "Western Blot: KIF2B Antibody - BSA Free [NBP3-05100] - Western blot analysis of various lysates, using KIF2B Rabbit pAb (A6480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.")
![Western Blot: KIF2B Antibody [NBP2-87691] - WB Suggested Anti-KIF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate](http://images.novusbio.com/fullsize/KIF2B-Antibody-Western-Blot-NBP2-87691-img0001.jpg "Western Blot: KIF2B Antibody [NBP2-87691] - WB Suggested Anti-KIF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate")
![Western Blot: KIF2B Overexpression Lysate (Adult Normal) [NBP2-06003] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIF2B.](http://images.novusbio.com/fullsize/KIF2B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06003-img0001.jpg "Western Blot: KIF2B Overexpression Lysate (Adult Normal) [NBP2-06003] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIF2B.")