| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26135-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26574-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14043 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26125-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14043 | |||
![Immunohistochemistry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Image of HIST1H3A [Monomethyl Lys9] Antibody (13E1) diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB.](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-262202414471558.jpg "Immunohistochemistry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Image of HIST1H3A [Monomethyl Lys9] Antibody (13E1) diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Staining of A549 with HIST1H3A [Monomethyl Lys9] Antibody (13E1) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 489-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-282202412493612.jpg)
![Flow Cytometry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Overlay Peak curve showing A549 cells stained with HIST1H3A [Monomethyl Lys9] Antibody (13E1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-282202414183677.jpg)
![Western Blot: HIST1H3A Antibody (S06-4I8) [NBP3-14941] - Western blot detection of HIST1H3A in Jurkat, THP-1, C6, 3T3 cell lysates using HIST1H3A Rabbit mAb (1:1000 diluted). Predicted band size: 15KDa. Observed band size: 17KDa.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-S06-4I8-Western-Blot-NBP3-14941-img0001.jpg "Western Blot: HIST1H3A Antibody (S06-4I8) [NBP3-14941] - Western blot detection of HIST1H3A in Jurkat, THP-1, C6, 3T3 cell lysates using HIST1H3A Rabbit mAb (1:1000 diluted). Predicted band size: 15KDa. Observed band size: 17KDa.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (S06-4I8) [NBP3-14941] - Immunohistochemistry of HIST1H3A in paraffin-embedded Human lung cancer tissue using HIST1H3A Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-S06-4I8-Immunohistochemistry-Paraffin-NBP3-14941-img0002.jpg)
![Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].](http://images.novusbio.com/fullsize/HIST1H3A-ac-Lys9-Antibody-464-Western-Blot-NBP2-43733-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [464] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/HIST1H3A-ac-Lys9-Antibody-464-Immunocytochemistry-Immunofluorescence-NBP2-43733-img0002.jpg)
![Immunohistochemistry: HIST1H3A [Formyl Lys122] Antibody (32C1) [NBP3-26574] - Image of HIST1H3A [Formyl Lys122] Antibody (32C1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.](http://images.novusbio.com/fullsize/nbp3-26574_rabbit-hist1h3a-formyl-lys-122-mab-32c1-262202414471535.jpg "Immunohistochemistry: HIST1H3A [Formyl Lys122] Antibody (32C1) [NBP3-26574] - Image of HIST1H3A [Formyl Lys122] Antibody (32C1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.")
![Immunohistochemistry: HIST1H3A [Formyl Lys122] Antibody (32C1) [NBP3-26574] - Image of HIST1H3A [Formyl Lys122] Antibody (32C1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.](http://images.novusbio.com/fullsize/nbp3-26574_rabbit-hist1h3a-formyl-lys-122-mab-32c1-26220241525332.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (rPHH3/6824) [NBP3-13802] - Formalin-fixed, paraffin-embedded human bladder stained with HIST1H3A [p Ser10] antibody (rPHH3/6824).](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-rPHH3-6824-Immunohistochemistry-Paraffin-NBP3-13802-img0001.jpg "Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (rPHH3/6824) [NBP3-13802] - Formalin-fixed, paraffin-embedded human bladder stained with HIST1H3A [p Ser10] antibody (rPHH3/6824).")
![Western Blot Analysis of Human MCF-7 lysate using HIST1H3A [p Ser10] Antibody (rPHH3/6824).](http://images.novusbio.com/fullsize/nbp3-13802_mouse-hist1h3a-p-ser-10-mab-rphh3-6824-26202521345241.jpg)
![Western Blot: HIST1H3A [Dimethyl Lys36] Antibody (1488) [NBP2-43763] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody [GT1488] (NBP2-43763) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43763_mouse-monoclonal-hist1h3a-dimethyl-lys36-antibody-1488-9102024201462.jpg "Western Blot: HIST1H3A [Dimethyl Lys36] Antibody (1488) [NBP2-43763] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody [GT1488] (NBP2-43763) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: HIST1H3A Overexpression Lysate (Adult Normal) [NBP2-07992] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H3A.](http://images.novusbio.com/fullsize/HIST1H3A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07992-img0001.jpg "Western Blot: HIST1H3A Overexpression Lysate (Adult Normal) [NBP2-07992] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H3A.")
![Immunohistochemistry: HIST1H3A Antibody (35C11) - K27M mutant [NBP3-26125] - Image of HIST1H3A Antibody (35C11) - K27M mutant diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.08% DAB.](http://images.novusbio.com/fullsize/nbp3-26125_rabbit-hist1h3a-mab-35c11-k27m-mutant-2722024856316.jpg "Immunohistochemistry: HIST1H3A Antibody (35C11) - K27M mutant [NBP3-26125] - Image of HIST1H3A Antibody (35C11) - K27M mutant diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.08% DAB.")