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![Western Blot: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts of various cell lines, using GSK-3 alpha antibody (NBP3-15579) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Western-Blot-NBP3-15579-img0002.jpg "Western Blot: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts of various cell lines, using GSK-3 alpha antibody (NBP3-15579) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts from normal (control) and GSK-3 alpha knockout (KO) HeLa cells, using GSK-3 alpha antibody (18655) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Knockout-Validated-NBP3-15579-img0001.jpg)
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0003.jpg "Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 and HeLa cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0002.jpg)
![Immunoprecipitation: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug GSK-3 alpha/beta pAb (NBP3-05443). Western blot was performed from the immunoprecipitate using GSK-3 alpha/beta pAb (NBP3-05443) at a dilution of 1:1000. 293T cells were treated by Insulin (100 nM) at 37C for 10 minutes after serum-starvation overnight.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Immunoprecipitation-NBP3-05443-img0001.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216] Antibody (M132) [NBP3-23367] - Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3 beta (lanes 1 & 2) or anti-GSK-3 alpha/beta (Y279/Y216) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23367_mouse-gsk-3-alpha-beta-p-tyr216-mab-m132-19920231856920.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216] Antibody (M132) [NBP3-23367] - Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3 beta (lanes 1 & 2) or anti-GSK-3 alpha/beta (Y279/Y216) (lanes 3 & 4).")
![Western Blot: GSK-3 alpha Antibody (S07-3A2) [NBP3-15036] - Western blot detection of GSK-3 alpha in K562, Rat Brain, Hela cell lysates using GSK-3 alpha Rabbit mAb (1:1000 diluted). Predicted band size: 51kDa. Observed band size: 51kDa.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-S07-3A2-Western-Blot-NBP3-15036-img0001.jpg "Western Blot: GSK-3 alpha Antibody (S07-3A2) [NBP3-15036] - Western blot detection of GSK-3 alpha in K562, Rat Brain, Hela cell lysates using GSK-3 alpha Rabbit mAb (1:1000 diluted). Predicted band size: 51kDa. Observed band size: 51kDa.")
![Western Blot: GSK-3 alpha/beta Antibody (S08-8G7) [NBP3-19938] - Western blot detection of GSK-3 alpha/beta in Jurkat, Rat Brain, C6, CHO-K1, Hela cell lysates using NBP3-19938 (1:1000 diluted). Predicted band size: 51, 47 kDa. Observed band size: 51, 47kDa.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-Antibody-S08-8G7-Western-Blot-NBP3-19938-img0001.jpg "Western Blot: GSK-3 alpha/beta Antibody (S08-8G7) [NBP3-19938] - Western blot detection of GSK-3 alpha/beta in Jurkat, Rat Brain, C6, CHO-K1, Hela cell lysates using NBP3-19938 (1:1000 diluted). Predicted band size: 51, 47 kDa. Observed band size: 51, 47kDa.")
![Western Blot: GSK3 alpha/beta [p Ser9] Antibody [NB300-741] - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/GSK3-alpha-beta-[p-Ser9]-Antibody-Western-Blot-NB300-741-img0001.jpg "Western Blot: GSK3 alpha/beta [p Ser9] Antibody [NB300-741] - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).")
![Western Blot: GSK3 alpha/beta Antibody [NBP2-29873] - HEK293 cell lysate was probed with anti-GSK3alpha /Beta (1:5,000 dilution). Arrows indicate GSK3alpha (~51kDa) and GSK3Beta (~46kDa).](http://images.novusbio.com/fullsize/GSK3-alpha-beta-Antibody-Western-Blot-NBP2-29873-img0001.jpg "Western Blot: GSK3 alpha/beta Antibody [NBP2-29873] - HEK293 cell lysate was probed with anti-GSK3alpha /Beta (1:5,000 dilution). Arrows indicate GSK3alpha (~51kDa) and GSK3Beta (~46kDa).")
![Western Blot: GSK3 alpha Overexpression Lysate (Adult Normal) [NBL1-11361] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSK3 alpha.](http://images.novusbio.com/fullsize/GSK3-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11361-img0002.jpg "Western Blot: GSK3 alpha Overexpression Lysate (Adult Normal) [NBL1-11361] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSK3 alpha.")
![Western Blot: GSK-3 alpha Antibody (6G12C2) [NB110-87049] - Analysis using GSK3 alpha mouse mAb against truncated GSK3 alpha recombinant protein (1)and Hela cell lysate (2).](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-6G12C2-Western-Blot-NB110-87049-img0003.jpg "Western Blot: GSK-3 alpha Antibody (6G12C2) [NB110-87049] - Analysis using GSK3 alpha mouse mAb against truncated GSK3 alpha recombinant protein (1)and Hela cell lysate (2).")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NBP2-97994] - Immunofluorescence staining of GSK-3 alpha in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human GSK-3 alpha polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97994-img0001.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NBP2-97994] - Immunofluorescence staining of GSK-3 alpha in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human GSK-3 alpha polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry: GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) [NBP3-15149] - IHC image of GSK-3 alpha antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minute at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/GSK-3-alpha-p-Tyr216-p-Tyr279-Antibody-4A5-Immunohistochemistry-NBP3-15149-img0001.jpg "Immunohistochemistry: GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) [NBP3-15149] - IHC image of GSK-3 alpha antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minute at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![ELISA: Rat GSK-3 alpha ELISA Kit (Colorimetric) [NBP3-31965] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31965_rat-gsk-3-alpha-elisa-kit-colorimetric-2110202415302959.png "ELISA: Rat GSK-3 alpha ELISA Kit (Colorimetric) [NBP3-31965] - Standard Curve Reference")