| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43815 | |||
| BSA Free | NBP2-46629-25ul | |||
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2247-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52471-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89801-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79938 | |||
![Western Blot: GAD2/GAD65 Antibody [NB110-41528] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:4000 antibody2. 1:8000 antibody3. Negative Control](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NB110-41528-img0005.jpg "Western Blot: GAD2/GAD65 Antibody [NB110-41528] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:4000 antibody2. 1:8000 antibody3. Negative Control")
![Immunohistochemistry: GAD2/GAD65 Antibody [NB110-41528] - Formalin fixed, paraffin-embedded Rat pancreas tissue sections were stained with 1:1,000 Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit followed by 20 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 20 ug/mL ExtrAvidin-Peroxidase.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-NB110-41528-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB110-41528] - GAD2/GAD65 labelling in Golden hamster hippocampal fomation. Inset: hilus & dentate gyrus. Image from verified customer review.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NB110-41528-img0003.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0009.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse midbrain shows moderate positivity in GABergic neural fibers in the substantia nigra.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0014.jpg)
![Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0008.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat basal forebrain shows moderate immunoreactivity in GABAergic fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0001.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse substantia nigra shows moderate positivity in GABAergic fibers in pars reticulata.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0002.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat globus pallidus shows moderate positivity in GABAergic neural fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebellum shows moderate immunoreactivity in GABAergic fibers in granular and molecular layers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0005.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebral cortex shows moderate to strong positivity in GABAergic neuronal processes.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0011.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human pancreas shows moderate cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0012.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0013.jpg)
![Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NBP1-33284-img0012.jpg "Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284] - RT2 xenograft. GAD65 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-33284-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33284-img0008.jpg)
![GAD65 antibody detects GAD65 protein by immunofluorescent analysis.</br>Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: GAD65 stained by GAD65 antibody (NBP1-33284) diluted at 1:500.</br>Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33284_rabbit-polyclonal-gad2-gad65-antibody-immunocytochemistry-immunofluorescence-2611202518365910.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0003.jpg "Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Flow-Cytometry-NBP2-52471-img0001.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0002.jpg)
![ELISA: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-ELISA-NBP2-52471-img0004.jpg)
![SDS-Page: GAD2/GAD65 Recombinant Protein [H00002572-P02]](http://images.novusbio.com/fullsize/GAD2-GAD65-Recombinant-Protein-SDS-Page-H00002572-P02-img0001.jpg "SDS-Page: GAD2/GAD65 Recombinant Protein [H00002572-P02]")
![Western Blot: GAD2/GAD65 Antibody [NB100-1506] - (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NB100-1506-img0001.jpg "Western Blot: GAD2/GAD65 Antibody [NB100-1506] - (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-1506_goat-polyclonal-gad2-gad65-antibody-16520239183338.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] - (6ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-1506_goat-polyclonal-gad2-gad65-antibody-1652023922513.jpg)
![ELISA: Mouse GAD2/GAD65 ELISA Kit (Colorimetric) [NBP2-67968] - Samples were spiked with high concentrations of Mouse GAD2/GAD65 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67968_mouse-gad2-gad65-elisa-kit-colorimetric-13220241601216.jpg "ELISA: Mouse GAD2/GAD65 ELISA Kit (Colorimetric) [NBP2-67968] - Samples were spiked with high concentrations of Mouse GAD2/GAD65 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse GAD2/GAD65 ELISA Kit (Colorimetric) [NBP2-67968] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67968_mouse-gad2-gad65-elisa-kit-colorimetric-432024924084.jpg)
![ELISA: Human GAD2/GAD65 ELISA Kit (Colorimetric) [NBP3-18768] - Samples were spiked with high concentrations of Human GAD2/GAD65 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18768_human-gad2-gad65-elisa-kit-colorimetric-13220241613015.jpg "ELISA: Human GAD2/GAD65 ELISA Kit (Colorimetric) [NBP3-18768] - Samples were spiked with high concentrations of Human GAD2/GAD65 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human GAD2/GAD65 ELISA Kit (Colorimetric) [NBP3-18768] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18768_human-gad2-gad65-elisa-kit-colorimetric-432024924041.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human skeletal muscle shows no granular cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-89801-img0006.jpg "Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human skeletal muscle shows no granular cytoplasmic positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-89801-img0003.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-89801-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human pancreas shows strong granular cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-89801-img0005.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804] - Formalin-fixed, paraffin-embedded human Pancreas stained with GAD2/GAD65 Antibody (GAD2/1960).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-GAD2-1960-Immunohistochemistry-Paraffin-NBP2-79804-img0001.jpg "Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804] - Formalin-fixed, paraffin-embedded human Pancreas stained with GAD2/GAD65 Antibody (GAD2/1960).")
![Protein Array: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD2/GAD65 Antibody (GAD2/1960) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-GAD2-1960-Protein-Array-NBP2-79804-img0002.jpg)
