MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." alt="Feline peripheral blood mononuclear cells were treated with PMA and Ca2+ionomycin, then stained with Mouse Anti-Feline Fas/ TNFRSF6/CD95 Mono-clonal Antibody (Catalog # MAB2267, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2267-SP | |||
| Carrier Free | FAB2267V-100UG | |||
| Carrier Free | FAB2267U-100UG | |||
| Carrier Free | FAB2267T-100UG | |||
| Carrier Free | FAB2267S-100UG | |||
| Carrier Free | FAB2267R-100UG | |||
| Carrier Free | FAB2267N-100UG | |||
| Carrier Free | FAB2267G-100UG | |||
326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB144-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13078-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81113-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB22671-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47918-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-61007-0.025mg | |||
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0004.jpg "Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line")
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fig. 1. Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody Green - anti-CD95 (LT95) A. JURKAT human peripheral blood T cell leukemia cell line B. RAMOS human Burkitt lymphoma cell line C. CEM human leukemia cell line D. MOLT-4 human acute lymphoblastic T cell leukemia cell line.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0001.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - A surface stain was performed on HeLa with Fas/TNFRSF6/CD95 Antibody (LT95) NB500-503AF647 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0003.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Using the Allophycocyanin direct conjugate Surface staining of human peripheral blood cells with anti-CD95 (LT95) APC.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0002.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 3: Western blot analysis using Fas/TNFRSF6/CD95 mouse monoclonal antibody against Hela (1), Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-Western-Blot-NBP2-13078-img0003.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 3: Western blot analysis using Fas/TNFRSF6/CD95 mouse monoclonal antibody against Hela (1), Jurkat (2) cell lysate.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 4: Flow cytometric analysis of Hela cells using Fas/TNFRSF6/CD95 mouse monoclonal antibody (green) and negative control (red).](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-Flow-Cytometry-NBP2-13078-img0004.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 2: Western blot analysis using Fas/TNFRSF6/CD95 monoclonal antibody against human Fas/TNFRSF6/CD95 (AA: 87-278) recombinant protein. (Expected MW is 47.2 kDa)](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-Western-Blot-NBP2-13078-img0002.jpg)
![ELISA: Fas Receptor/TNFRSF6/CD95 Antibody (4F8D6) [NBP2-13078] - Fas/TNFRSF6/CD95 Antibody (4F8D6) [NBP2-13078] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-ELISA-NBP2-13078-img0001.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (5D10O3) [NBP3-15788] - Western blot analysis of extracts of various cell lines, using Fas/TNFRSF6/CD95 antibody (NBP3-15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-5D10O3-Western-Blot-NBP3-15788-img0003.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (5D10O3) [NBP3-15788] - Western blot analysis of extracts of various cell lines, using Fas/TNFRSF6/CD95 antibody (NBP3-15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Fas/TNFRSF6/CD95 Antibody (5D10O3) [NBP3-15788] - Immunohistochemistry of paraffin-embedded rat kidney using Fas/TNFRSF6/CD95 antibody (NBP3-15788) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-5D10O3-Immunohistochemistry-Paraffin-NBP3-15788-img0002.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Western-Blot-NBP2-81113-img0002.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Flow-cytometry using the Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (NBP2-81113, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Flow-Cytometry-NBP2-81113-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric - Azide and BSA Free [NBP2-81113] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with NBP2-81113 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81113, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81113_rabbit-monoclonal-fas-tnfrsf6-cd95-antibody-r-125224-chimeric-74202522141233.png)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody (MM0041-12J3) [NB110-61007] - Fas Antibody (MM0041-12J3) [NB110-61007] - Formalin fixed and paraffin embedded normal human placenta tissue is subjected to mouse anti human Fas.](http://images.novusbio.com/fullsize/Fas-Antibody-MM0041-12J3-Immunohistochemistry-Paraffin-NB110-61007-img0002.jpg "Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody (MM0041-12J3) [NB110-61007] - Fas Antibody (MM0041-12J3) [NB110-61007] - Formalin fixed and paraffin embedded normal human placenta tissue is subjected to mouse anti human Fas.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (EOS9.1) - Low Endotoxin, Azide and BSA Free [NBP3-09092] - Separation of anti-CD95 (EOS9.1) antibody stimulated CEM cells (24 hours, 1 ug/ml, red-filled) from nonstimulated CEM cells (black-dashed) in flow cytometry analysis CEM cell suspension stained using ApoFlowEx kit (ED7044).](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-EOS9-1-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09092-img0002.jpg "Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (EOS9.1) - Low Endotoxin, Azide and BSA Free [NBP3-09092] - Separation of anti-CD95 (EOS9.1) antibody stimulated CEM cells (24 hours, 1 ug/ml, red-filled) from nonstimulated CEM cells (black-dashed) in flow cytometry analysis CEM cell suspension stained using ApoFlowEx kit (ED7044).")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (EOS9.1) - Low Endotoxin, Azide and BSA Free [NBP3-09092] - Separation of anti-CD95 (EOS9.1) antibody stimulated Jurkat cells (24 hours, 4 ug/ml, red-filled) from nonstimulated Jurkat cells (black-dashed) in flow cytometry analysis of Jurkat cellular suspension stained using ApoFlowEx kit (ED7044).](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-EOS9-1-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09092-img0001.jpg)
