NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="FABP6 was detected in immersion fixed T84 human colon carcinoma cell line using Goat Anti-Human FABP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3880-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83129-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99577-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90127-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35006-1mg | |||
| Carrier-Free | NBP2-35006-100ug | |||
| Carrier-Free | NBP2-35006-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90126-50ul | |||
![Western Blot: FABP6 Antibody [NBP1-32482] - Sample(30 ug whole cell lysate)A:HeLa S3 B:Hep G2 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FABP6-Antibody-Western-Blot-NBP1-32482-img0002.jpg "Western Blot: FABP6 Antibody [NBP1-32482] - Sample(30 ug whole cell lysate)A:HeLa S3 B:Hep G2 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: FABP6 Antibody [NBP1-32482] - Human colon carcinoma. ILBP antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/FABP6-Antibody-Immunohistochemistry-Paraffin-NBP1-32482-img0003.jpg)
![Immunohistochemistry-Paraffin: FABP6 Antibody [NBP1-83129] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/FABP6-Antibody-Immunohistochemistry-Paraffin-NBP1-83129-img0007.jpg "Immunohistochemistry-Paraffin: FABP6 Antibody [NBP1-83129] - Staining of human small intestine shows high expression.")
![Immunohistochemistry-Paraffin: FABP6 Antibody [NBP1-83129] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/FABP6-Antibody-Immunohistochemistry-Paraffin-NBP1-83129-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP6 Antibody [NBP1-83129] - Staining in human small intestine and liver tissues using anti-FABP6 antibody. Corresponding FABP6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP6-Antibody-Immunohistochemistry-Paraffin-NBP1-83129-img0008.jpg)
![Immunohistochemistry-Paraffin: FABP6 Antibody [NBP2-99577] - Staining FABP6 in rat small intestine with rabbit polyclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FABP6-Antibody-Immunohistochemistry-Paraffin-NBP2-99577-img0002.jpg "Immunohistochemistry-Paraffin: FABP6 Antibody [NBP2-99577] - Staining FABP6 in rat small intestine with rabbit polyclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: FABP6 Antibody [NBP2-99577] - Staining FABP6 in mouse small intestine with rabbit polyclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FABP6-Antibody-Immunohistochemistry-Paraffin-NBP2-99577-img0001.jpg)
![Flow Cytometry: FABP6 Antibody (201) [NBP2-90127] - Flow cytometric analysis of Human FABP6 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human FABP6, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/FABP6-Antibody-201-Flow-Cytometry-NBP2-90127-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: FABP6 Antibody (201) [NBP2-90127] - Flow cytometric analysis of Human FABP6 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human FABP6, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FABP6 Antibody (201) [NBP2-90127] - Immunofluorescence staining of Human FABP6 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90127 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90127_rabbit-monoclonal-fabp6-antibody-201-2352025162810.png)
![Western Blot: FABP6 Antibody (4A4) [H00002172-M01] - Analysis of FABP6 expression in transfected 293T cell line by FABP6 monoclonal antibody (M01), clone 4A4.Lane 1: FABP6 transfected lysate(14.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FABP6-Antibody-4A4-Western-Blot-H00002172-M01-img0003.jpg "Western Blot: FABP6 Antibody (4A4) [H00002172-M01] - Analysis of FABP6 expression in transfected 293T cell line by FABP6 monoclonal antibody (M01), clone 4A4.Lane 1: FABP6 transfected lysate(14.4 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: FABP6 Antibody (4A4) [H00002172-M01] - Detection limit for recombinant GST tagged FABP6 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FABP6-Antibody-4A4-Sandwich-ELISA-H00002172-M01-img0002.jpg)
![ELISA: Mouse FABP6 ELISA Kit (Colorimetric) [NBP2-82412] - Samples were spiked with high concentrations of Mouse FABP6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82412_mouse-fabp6-elisa-kit-colorimetric-132202416215385.jpg "ELISA: Mouse FABP6 ELISA Kit (Colorimetric) [NBP2-82412] - Samples were spiked with high concentrations of Mouse FABP6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse FABP6 ELISA Kit (Colorimetric) [NBP2-82412] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82412_mouse-fabp6-elisa-kit-colorimetric-4320249124847.jpg)
![ELISA: Human FABP6 ELISA Kit (Colorimetric) [NBP2-82508] - Samples were spiked with high concentrations of Human FABP6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82508_human-fabp6-elisa-kit-colorimetric-13220241681462.jpg "ELISA: Human FABP6 ELISA Kit (Colorimetric) [NBP2-82508] - Samples were spiked with high concentrations of Human FABP6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human FABP6 ELISA Kit (Colorimetric) [NBP2-82508] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82508_human-fabp6-elisa-kit-colorimetric-4320248595138.jpg)
![Western Blot: FABP6 Overexpression Lysate (Adult Normal) [NBL1-10422] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FABP6.](http://images.novusbio.com/fullsize/FABP6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10422-img0002.jpg "Western Blot: FABP6 Overexpression Lysate (Adult Normal) [NBL1-10422] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FABP6.")
![SDS-Page: Human FABP6 Protein [NBP2-35006]](http://images.novusbio.com/fullsize/Human-FABP6-Protein-SDS-Page-NBP2-35006-img0001.jpg "SDS-Page: Human FABP6 Protein [NBP2-35006]")
