| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90641-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26661-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85743-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79287-5Plates | |||
![Western Blot: CEACAM1/CD66a Antibody (PSH0-78) [NBP3-32177] - Western blot analysis of CEACAM1/CD66a on different lysates with Rabbit anti-CEACAM1/CD66a antibody (NBP3-32177) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution. Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32177) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32177_rabbit-ceacam1-cd66a-mab-psh0-78-27820241631656.jpg "Western Blot: CEACAM1/CD66a Antibody (PSH0-78) [NBP3-32177] - Western blot analysis of CEACAM1/CD66a on different lysates with Rabbit anti-CEACAM1/CD66a antibody (NBP3-32177) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution. Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32177) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CEACAM1/CD66a Antibody (PSH0-78) [NBP3-32177] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CEACAM1/CD66a antibody (NBP3-32177) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32177) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32177_rabbit-ceacam1-cd66a-mab-psh0-78-298202414262654.jpg)
![Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (117) [NBP2-90641] - Immunofluorescence staining of mouse CEACAM1 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CEACAM1 monoclonal antibody (1:60) overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CEACAM1-CD66a-Antibody-117-Immunocytochemistry-Immunofluorescence-NBP2-90641-img0001.jpg "Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (117) [NBP2-90641] - Immunofluorescence staining of mouse CEACAM1 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CEACAM1 monoclonal antibody (1:60) overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).")
![Flow Cytometry: CEACAM1/CD66a Antibody (117) [NBP2-90641] - Flow cytometric analysis of Mouse CEACAM1(CD66a) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CEACAM1(CD66a), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CEACAM1-CD66a-Antibody-117-Flow-Cytometry-NBP2-90641-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Staining of CEACAM1 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CEACAM1 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/CEACAM1-CD66a-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-89666-img0002.jpg "Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Staining of CEACAM1 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CEACAM1 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).")
![Flow Cytometry: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Analysis of Human CEACAM1(CD66a) expression in HT29 cells. The cells were harvested and stained with purified Rabbit anti-human CEACAM1, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD (TM) FACSCalibur flow cytometry system.](http://images.novusbio.com/fullsize/CEACAM1-CD66a-Antibody-103-Flow-Cytometry-NBP2-89666-img0001.jpg)
![Immunohistochemistry: CEACAM1/CD66a Antibody (10H8) [NBP3-26661] - Image of CEACAM1/CD66a Antibody (10H8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26661_rabbit-ceacam1-cd66a-mab-10h8-26220241522426.jpg "Immunohistochemistry: CEACAM1/CD66a Antibody (10H8) [NBP3-26661] - Image of CEACAM1/CD66a Antibody (10H8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: CEACAM1/CD66a Antibody (10H8) [NBP3-26661] - Image of CEACAM1/CD66a Antibody (10H8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26661_rabbit-ceacam1-cd66a-mab-10h8-262202415475641.jpg)
![Immunohistochemistry-Paraffin: CEACAM3 Antibody [NBP1-85743] - Staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.](http://images.novusbio.com/fullsize/CEACAM3-Antibody-Immunohistochemistry-Paraffin-NBP1-85743-img0003.jpg "Immunohistochemistry-Paraffin: CEACAM3 Antibody [NBP1-85743] - Staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.")
![Western Blot: CEACAM1 Overexpression Lysate (Adult Normal) [NBP2-10255] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CEACAM1.](http://images.novusbio.com/fullsize/CEACAM1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10255-img0001.jpg "Western Blot: CEACAM1 Overexpression Lysate (Adult Normal) [NBP2-10255] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CEACAM1.")
![Sandwich ELISA: CEACAM1/CD66a Antibody Pair [HRP] [NBP2-79287] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/CEACAM1-CD66a-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79287-img0001.jpg "Sandwich ELISA: CEACAM1/CD66a Antibody Pair [HRP] [NBP2-79287] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")