| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70544 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26637-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35383-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70542 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35404-20ul | |||
![Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Western-Blot-NBP2-01151-img0024.jpg "Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.")
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-OTI3F11-Immunohistochemistry-NBP2-01151-img0026.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Flow-Cytometry-NBP2-01151-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0016.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human breast tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0019.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0020.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0021.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0022.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human tonsil using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0023.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-OTI3F11-Immunohistochemistry-NBP2-01151-img0025.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Flow-Cytometry-NBP2-01151-img0013.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Western-Blot-NBP2-75461-img0008.jpg "Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0007.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of SH-SY-5Y cells with Cytochrome P450 17A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Flow-Cytometry-NBP2-75461-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human breast tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0006.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842] - (0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NB100-2842-img0004.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842] - (0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NB100-2842] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NB100-2842-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining in human testis and pancreas tissues.. Corresponding CYP17A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining in human testis and pancreas tissues.. Corresponding CYP17A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome P450 17A1 Antibody [NBP2-13892] - Analysis in human adrenal gland tissue.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-13892-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human pancreas shows no positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0011.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Western-Blot-NBP3-16541-img0002.jpg "Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Immunofluorescence analysis of HeLa cells using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Immunocytochemistry-Immunofluorescence-NBP3-16541-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Immunofluorescence analysis of HeLa cells using Cytochrome P450 17A1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16541_rabbit-cytochrome-p450-17a1-mab-5d2h1-5220251265350.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [Cytochrome P450 17A1] - Immunofluorescence analysis of HeLa cells using Cytochrome P450 17A1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16541_rabbit-cytochrome-p450-17a1-mab-5d2h1-52202517311043.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-2722024831378.jpg "Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Positive Western Blot detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate. All lanes: Cytochrome P450 17A1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa Observed band size: 58 kDa](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-28220241140920.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Staining of Hela Cells with Cytochrome P450 17A1 Antibody (5A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-282202412475149.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-16077-img0003.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.")
![Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-16077-img0001.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-16077-img0002.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35383] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35383_rabbit-cytochrome-p450-17a1-pab-1012202411592881.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35383] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: Cytochrome P450 17A1 Antibody (3F12) [NBP2-46418] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 .](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F12-Western-Blot-NBP2-46418-img0001.jpg "Western Blot: Cytochrome P450 17A1 Antibody (3F12) [NBP2-46418] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 .")
![Western Blot: Cytochrome P450 17A1 Antibody (OTI3F12) - Azide and BSA Free [NBP2-70542] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 .](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-OTI3F12-Azide-and-BSA-Free-Western-Blot-NBP2-70542-img0001.jpg "Western Blot: Cytochrome P450 17A1 Antibody (OTI3F12) - Azide and BSA Free [NBP2-70542] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 .")
![Western Blot: Cytochrome P450 17A1 Overexpression Lysate (Adult Normal) [NBL1-09673] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytochrome P450 17A1.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09673-img0002.jpg "Western Blot: Cytochrome P450 17A1 Overexpression Lysate (Adult Normal) [NBL1-09673] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytochrome P450 17A1.")
![Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35404] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35404_rabbit-cytochrome-p450-17a1-pab-10122024120623.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35404] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")