| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82793-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82792-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92369-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06071-50ul | |||
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82793] - Staining of human cerebellum, endometrium, kidney and testis using Anti-CLCC1 antibody NBP1-82793 (A) shows similar protein distribution across tissues to independent antibody NBP1-82792 (B).](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82793-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82793] - Staining of human cerebellum, endometrium, kidney and testis using Anti-CLCC1 antibody NBP1-82793 (A) shows similar protein distribution across tissues to independent antibody NBP1-82792 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CLCC1 Antibody [NBP1-82793] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82793-img0009.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82793] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82793-img0020.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82793] - Staining of human cerebellum shows weak positivity in neuronal processes.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82793-img0016.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82793] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82793-img0018.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82793] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82793-img0019.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CLCC1 Antibody [NBP1-82793] - CLCC1 siRNA interference in ARPE19 cells. Western Blot of SiRNA treated ARPE19 cell lysates probed with CLCC1 antibodies. Lane 1, untransfected lysate; lane 2, transfected with RNAi Max transfection reagent only; lanes 3-12, Increasing CLCC1 and control siRNA amounts from 10 pmol (lanes 3,4) to 70 pmol (lanes 11, 12). CLCC1 proteins migrate at the predicted MW of 67 kDa. The blot shows a dose dependent reduction of CLCC1 protein expression in CLCC1 but not control siRNA treated ARPE19 cells to about 20% of normal. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007504), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Knockdown-Validated-NBP1-82793-img0021.jpg)
![Western Blot: CLCC1 Antibody [NBP1-82793] - CLCC1 siRNA interference in ARPE19 cells.(a) Western Blot of SiRNA treated ARPE19 cell lysates probed with CLCC1 antibodies. Lane 1, untransfected lysate; lane 2, transfected with RNAi Max transfection reagent only; lanes 3–12, Increasing CLCC1 & control siRNA amounts from 10 pmol (lanes 3,4) to 70 pmol (lanes 11, 12). CLCC1 proteins migrate at the predicted MW of 67 kDa. The blot shows a dose dependent reduction of CLCC1 protein expression in CLCC1 but not control siRNA treated ARPE19 cells to about 20% of normal. (b) TUNEL assay after siRNA transfection. Left: TUNEL-positive apoptotic cells (green), Second: CLCC1 (red), Third: DAPI (blue, nucleus). Although there is some variation in intensity of individual cells, probably based on cell size, shape, & orientation, staining for CLCC1 is lower overall in the CLCC1 siRNA treated cells than the control siRNA, control, or DNase 1 cells, consistent with the Western blot in Fig 5a. About 10% of CLCC1 siRNA transfected cells were apoptotic (arrows) but there is minimal apoptosis in control siRNA or untransfected cells. DNase I treated cells were 100% TUNEL-positive. Overlays of images from the first three columns are shown in the right column labeled Merged. Scale Bar: 20 μm. (c) Down regulation of CLCC1 induced apoptosis in nearly 10% of the cells (*** P<0.0001, t = 14.63) as compared to approximately 1% of cells treated with the control siRNA & less than 1% of untreated cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30157172), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82793_rabbit-polyclonal-clcc1-antibody-310202416114758.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82792] - Staining of human cerebellum, endometrium, kidney and testis using Anti-CLCC1 antibody NBP1-82792 (A) shows similar protein distribution across tissues to independent antibody NBP1-82793 (B).](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82792-img0015.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82792] - Staining of human cerebellum, endometrium, kidney and testis using Anti-CLCC1 antibody NBP1-82792 (A) shows similar protein distribution across tissues to independent antibody NBP1-82793 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CLCC1 Antibody [NBP1-82792] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum & vesicles.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82792-img0007.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82792] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82792-img0018.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82792] - Staining of human cerebellum shows weak positivity in neuronal processes.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82792-img0014.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82792] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82792-img0016.jpg)
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-82792] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82792-img0017.jpg)
![Western Blot: CLCC1 Antibody [NBP2-92369] - Analysis of extracts of various cell lines, using CLCC1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Western-Blot-NBP2-92369-img0001.jpg "Western Blot: CLCC1 Antibody [NBP2-92369] - Analysis of extracts of various cell lines, using CLCC1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP2-92369] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using CLCC1 Rabbit pAb (NBP2-92369) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-92369-img0002.jpg)
![Western Blot: CLCC1 Antibody [NBP1-59834] - HepG2 cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/CLCC1-Antibody-Western-Blot-NBP1-59834-img0001.jpg "Western Blot: CLCC1 Antibody [NBP1-59834] - HepG2 cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: CLCC1 Antibody [NBP1-59834] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunohistochemistry-Paraffin-NBP1-59834-img0003.jpg)
![Western Blot: CLCC1 Antibody [NBP3-06071] - Anti-CLCC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HT29 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa Observed band size:62 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/CLCC1-Antibody-Western-Blot-NBP3-06071-img0001.jpg "Western Blot: CLCC1 Antibody [NBP3-06071] - Anti-CLCC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HT29 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa Observed band size:62 kDa (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: CLCC1 Antibody [NBP3-06071] - CLCC1 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-CLCC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-CLCC1 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa Observed band size :62 kDa](http://images.novusbio.com/fullsize/CLCC1-Antibody-Immunoprecipitation-NBP3-06071-img0002.jpg)
![Western Blot:[NBP3-29556]-Detection of human CLCC1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLCC1 antibody used for WB at 0. 04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29556_rabbit-clcc1-pab-2312025129520.jpg "Western Blot:[NBP3-29556]-Detection of human CLCC1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLCC1 antibody used for WB at 0. 04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: CLCC1 Overexpression Lysate (Adult Normal) [NBL1-09230] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLCC1.](http://images.novusbio.com/fullsize/CLCC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09230-img0002.jpg "Western Blot: CLCC1 Overexpression Lysate (Adult Normal) [NBL1-09230] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLCC1.")
![Immunoprecipitation:[NBP3-18374]-Detection of human CLCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CLCC1 antibody used for IP at 6 µg per reaction. CLCC1 was also immunoprecipitated by rabbit anti-CLCC1 antibody. For blotting immunoprecipitated CLCC1, was used at 0. 4 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-18374_rabbit-clcc1-pab-23120251211250.jpg "Immunoprecipitation:[NBP3-18374]-Detection of human CLCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CLCC1 antibody used for IP at 6 µg per reaction. CLCC1 was also immunoprecipitated by rabbit anti-CLCC1 antibody. For blotting immunoprecipitated CLCC1, was used at 0. 4 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")