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![Western Blot: VE-Cadherin Antibody (MM0012-8A03) - Azide and BSA Free [NB110-60978] - Lysates of HUVECs with Mouse anti-Human VE-Cadherin (MM0012-8A03) antibody.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-MM0012-8A03-Azide-and-BSA-Free-Western-Blot-NB110-60978-img0003.jpg "Western Blot: VE-Cadherin Antibody (MM0012-8A03) - Azide and BSA Free [NB110-60978] - Lysates of HUVECs with Mouse anti-Human VE-Cadherin (MM0012-8A03) antibody.")
![Immunohistochemistry-Paraffin: VE-Cadherin Antibody (MM0012-8A03) [NB110-60978] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human VE-Cadherin](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-MM0012-8A03-Immunohistochemistry-Paraffin-NB110-60978-img0002.jpg)
![Immunohistochemistry-Paraffin: VE-Cadherin Antibody (MM0012-8A03) [NB110-60978] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human VE-Cadherin](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-MM0012-8A03-Immunohistochemistry-Paraffin-NB110-60978-img0001.jpg)
![Western Blot: VE-Cadherin Antibody (16B1) [NBP2-00342] - EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 ug/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Western-Blot-NBP2-00342-img0002.jpg "Western Blot: VE-Cadherin Antibody (16B1) [NBP2-00342] - EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 ug/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.")
![Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the Biotin conjugate of NBP2-00342. Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.25 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD144 (VE-Cadherin) Biotin (filled histogram)](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Flow-Cytometry-NBP2-00342-img0007.jpg)
![Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the Allophycocyanin conjugate of NBP2-00342. Staining of Human Umbilical Vein Endothelial Cells.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Flow-Cytometry-NBP2-00342-img0006.jpg)
![Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the PE conjugate of NBP2-00342.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Flow-Cytometry-NBP2-00342-img0008.jpg)
![Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-288202414191513.jpg "Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Immunocytochemistry analysis of HeLa cells labeling VE-Cadherin with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-29202410234272.jpg)
![Flow Cytometry: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Flow cytometric analysis of EA.hy926 cells labeling VE-Cadherin. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33088, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-2920241645321.jpg)
![Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody [NBP3-21223] - Staining of human cell line EFO-21 shows localization to nucleoplasm, nuclear membrane & plasma membrane.](http://images.novusbio.com/fullsize/nbp3-21223_rabbit-ve-cadherin-pab-45202315531342.jpg "Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody [NBP3-21223] - Staining of human cell line EFO-21 shows localization to nucleoplasm, nuclear membrane & plasma membrane.")
![Western Blot: VE-Cadherin Antibody [NBP3-21223] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21223_rabbit-ve-cadherin-pab-45202315493239.jpg)
![Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-048-Flow-Cytometry-NBP2-89498-img0001.jpg "Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody (048) [NBP2-89498] - Staining of CDH5 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89498 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89498_rabbit-monoclonal-ve-cadherin-antibody-048-235202521451786.png)
![Western Blot: VE-Cadherin [p Tyr731] Antibody [NBP2-54766] - Analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.](http://images.novusbio.com/fullsize/VE-Cadherin-[p-Tyr731]-Antibody-Western-Blot-NBP2-54766-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: VE-Cadherin [p Tyr731] Antibody [NBP2-54766] - Analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.")
![ELISA: Mouse VE-Cadherin ELISA Kit (Colorimetric) [NBP3-20089] - Samples were spiked with high concentrations of Mouse VE-Cadherin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-20089_mouse-ve-cadherin-elisa-kit-colorimetric-132202416102247.jpg "ELISA: Mouse VE-Cadherin ELISA Kit (Colorimetric) [NBP3-20089] - Samples were spiked with high concentrations of Mouse VE-Cadherin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse VE-Cadherin ELISA Kit (Colorimetric) [NBP3-20089] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-20089_mouse-ve-cadherin-elisa-kit-colorimetric-432024855052.jpg)
