| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89790-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5359-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98236-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90476-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99898-50ul | |||
![Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Analysis in human cell lines HEK293 and U2OS using Anti-C1QBP antibody. Corresponding C1QBP RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Western-Blot-NBP1-89790-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Analysis in human cell lines HEK293 and U2OS using Anti-C1QBP antibody. Corresponding C1QBP RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89790-img0011.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP1-89790-img0021.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Western-Blot-NBP1-89790-img0010.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP1-89790-img0018.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Staining of human lymph node shows strong granular cytoplasmic positivity in non-germinal and germinal center cells.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP1-89790-img0019.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP1-89790-img0020.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Western blot analysis of extracts of various cell lines, using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-4T1W10-Western-Blot-NBP3-15368-img0007.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Western blot analysis of extracts of various cell lines, using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Immunohistochemistry of paraffin-embedded rat ovary using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-4T1W10-Immunohistochemistry-Paraffin-NBP3-15368-img0006.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Immunohistochemistry of paraffin-embedded human colon carcinoma using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-4T1W10-Immunohistochemistry-Paraffin-NBP3-15368-img0001.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Immunohistochemistry of paraffin-embedded human esophageal cancer using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-4T1W10-Immunohistochemistry-Paraffin-NBP3-15368-img0002.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Immunohistochemistry of paraffin-embedded mouse heart using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-4T1W10-Immunohistochemistry-Paraffin-NBP3-15368-img0003.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Immunohistochemistry of paraffin-embedded mouse kidney using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-4T1W10-Immunohistochemistry-Paraffin-NBP3-15368-img0004.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody (4T1W10) [NBP3-15368] - Immunohistochemistry of paraffin-embedded rat kidney using HABP1/C1QBP/GC1q R antibody (NBP3-15368) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-4T1W10-Immunohistochemistry-Paraffin-NBP3-15368-img0005.jpg)
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (6N7U3) [NBP3-15368] - Immunohistochemistry analysis of HABP1/C1QBP/GC1q R in paraffin-embedded rat kidney tissue using HABP1/C1QBP/GC1q R Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15368_rabbit-habp1-c1qbp-gc1q-r-mab-6n7u3-72202512432085.jpg)
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (6N7U3) [NBP3-15368] - Immunohistochemistry analysis of HABP1/C1QBP/GC1q R in paraffin-embedded mouse kidney tissue using HABP1/C1QBP/GC1q R Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15368_rabbit-habp1-c1qbp-gc1q-r-mab-6n7u3-72202512432095.jpg)
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (6N7U3) [NBP3-15368] - Immunohistochemistry analysis of HABP1/C1QBP/GC1q R in paraffin-embedded human thyroid cancer tissue using HABP1/C1QBP/GC1q R Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15368_rabbit-habp1-c1qbp-gc1q-r-mab-6n7u3-72202512432073.jpg)
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (6N7U3) [NBP3-15368] - Immunohistochemistry analysis of HABP1/C1QBP/GC1q R in paraffin-embedded human colon tissue using HABP1/C1QBP/GC1q R Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15368_rabbit-habp1-c1qbp-gc1q-r-mab-6n7u3-7220251245391.jpg)
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (6N7U3) [NBP3-15368] - Immunohistochemistry analysis of HABP1/C1QBP/GC1q R in paraffin-embedded human liver tissue using HABP1/C1QBP/GC1q R Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15368_rabbit-habp1-c1qbp-gc1q-r-mab-6n7u3-72202512435310.jpg)
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (6N7U3) [NBP3-15368] - Immunohistochemistry analysis of HABP1/C1QBP/GC1q R in paraffin-embedded human colon carcinoma tissue using HABP1/C1QBP/GC1q R Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15368_rabbit-habp1-c1qbp-gc1q-r-mab-6n7u3-72202512435335.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody (A8F5-R) [NBP3-32377] - Western blot analysis of HABP1/C1QBP/GC1q R on different lysates with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32377) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat spleen tissue lysate (40 ug/Lane) Lane 9: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32377) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32377_mouse-habp1-c1qbp-gc1q-r-mab-a8f5-r-278202416285272.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody (A8F5-R) [NBP3-32377] - Western blot analysis of HABP1/C1QBP/GC1q R on different lysates with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32377) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat spleen tissue lysate (40 ug/Lane) Lane 9: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32377) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (A8F5-R) [NBP3-32377] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32377) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32377) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32377_mouse-habp1-c1qbp-gc1q-r-mab-a8f5-r-29820241435211.jpg)
![Immunocytochemistry/ Immunofluorescence: HABP1/C1QBP/GC1q R Antibody (A8F5-R) [NBP3-32377] - Immunocytochemistry analysis of HeLa cells labeling HABP1/C1QBP/GC1q R with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32377) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32377) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32377_mouse-habp1-c1qbp-gc1q-r-mab-a8f5-r-2920241192343.jpg)
![Flow Cytometry: HABP1/C1QBP/GC1q R Antibody (A8F5-R) [NBP3-32377] - Flow cytometric analysis of HeLa cells labeling HABP1/C1QBP/GC1q R. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32377, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32377_mouse-habp1-c1qbp-gc1q-r-mab-a8f5-r-2920241645325.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP2-98236] - Anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: Raji Whole Cell Lysate. Lane C: Mouse Spleen tissue Lysate. Lane D: Mouse Brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Western-Blot-NBP2-98236-img0006.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP2-98236] - Anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: Raji Whole Cell Lysate. Lane C: Mouse Spleen tissue Lysate. Lane D: Mouse Brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa")
![Immunocytochemistry/Immunofluorescence: HABP1/C1QBP/GC1q R Antibody [NBP2-98236] - Immunofluorescence staining of HABP1/C1QBP/GC1q R in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-MOUSE HABP1/C1QBP/GC1q R polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98236-img0001.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP2-98236] - Immunochemical staining of mouse HABP1/C1QBP/GC1q R in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP2-98236-img0005.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP2-98236] - Immunochemical staining of mouse HABP1/C1QBP/GC1q R in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP2-98236-img0004.jpg)
![Immunoprecipitation: HABP1/C1QBP/GC1q R Antibody [NBP2-98236] - HABP1/C1QBP/GC1q R was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunoprecipitation-NBP2-98236-img0002.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Western blot analysis of HABP1/C1QBP/GC1q R on different lysates with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat spleen tissue lysate (40 ug/Lane) Lane 9: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32376) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32376_mouse-habp1-c1qbp-gc1q-r-mab-a8f4-r-278202415242668.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Western blot analysis of HABP1/C1QBP/GC1q R on different lysates with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat spleen tissue lysate (40 ug/Lane) Lane 9: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32376) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32376) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32376_mouse-habp1-c1qbp-gc1q-r-mab-a8f4-r-298202414161836.jpg)
![Immunocytochemistry/ Immunofluorescence: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Immunocytochemistry analysis of HeLa cells labeling HABP1/C1QBP/GC1q R with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32376_mouse-habp1-c1qbp-gc1q-r-mab-a8f4-r-2920241114818.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP1-49960] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Western-Blot-NBP1-49960-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP1-49960] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.04 ug/ml.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody [NBP1-49960] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-C1QBP used at a dilution of 1:200 (1ug/ml). Detection: Vector ImmPACT NovaRED](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-NBP1-49960-img0006.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody (1G7) [NBP2-42691] - The recombinant protein(50ng) and cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant protein Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate Lane 4 : MCF7 cell lysate Lane 5 : Ramos cell lysate Lane 6 : 293T cell lysate Lane 7 : A549 cell lysate](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-1G7-Western-Blot-NBP2-42691-img0001.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody (1G7) [NBP2-42691] - The recombinant protein(50ng) and cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant protein Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate Lane 4 : MCF7 cell lysate Lane 5 : Ramos cell lysate Lane 6 : 293T cell lysate Lane 7 : A549 cell lysate")
![Immunocytochemistry/Immunofluorescence: HABP1/C1QBP/GC1q R Antibody (1G7) [NBP2-42691] - Analysis of C1QBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human C1QBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-1G7-Immunocytochemistry-Immunofluorescence-NBP2-42691-img0006.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody (106) [NBP2-90476] - Anti-HABP1/C1QBP/GC1q R rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-106-Western-Blot-NBP2-90476-img0002.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody (106) [NBP2-90476] - Anti-HABP1/C1QBP/GC1q R rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa")
![Immunoprecipitation: HABP1/C1QBP/GC1q R Antibody (106) [NBP2-90476] - Mouse HABP1/C1QBP/GC1q R was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-Mouse HABP1/C1QBP/GC1q R rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse HABP1/C1QBP/GC1q R rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-106-Immunoprecipitation-NBP2-90476-img0001.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP2-99898] - Anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Western-Blot-NBP2-99898-img0004.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP2-99898] - Anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: HABP1/C1QBP/GC1q R Antibody [NBP2-99898] - HABP1/C1QBP/GC1q R was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HABP1/C1QBP/GC1q R rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.](http://images.novusbio.com/fullsize/HABP1-C1QBP-GC1q-R-Antibody-Immunoprecipitation-NBP2-99898-img0003.jpg)